Development of reference transcriptomes for the major field insect pests of cowpea: a toolbox for insect pest management approaches in West Africa
Cowpea is a widely cultivated and major nutritional source of protein for many people that live in West Africa. Annual yields and longevity of grain storage is greatly reduced by feeding damage caused by a complex of insect pests that include the pod sucking bugs, Anoplocnemis curvipes Fabricius (He...
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Public Library of Science
2013
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/76659 |
| _version_ | 1855526846841487360 |
|---|---|
| author | Agunbiade, T.A. Sun, W. Coates, B.S. Djouaka, R.F. Tamo, Manuele Ba, Malick N. Binso-Dabire, L. Baoua, I. Olds, B.P. Pittendrigh, Barry Robert |
| author_browse | Agunbiade, T.A. Ba, Malick N. Baoua, I. Binso-Dabire, L. Coates, B.S. Djouaka, R.F. Olds, B.P. Pittendrigh, Barry Robert Sun, W. Tamo, Manuele |
| author_facet | Agunbiade, T.A. Sun, W. Coates, B.S. Djouaka, R.F. Tamo, Manuele Ba, Malick N. Binso-Dabire, L. Baoua, I. Olds, B.P. Pittendrigh, Barry Robert |
| author_sort | Agunbiade, T.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cowpea is a widely cultivated and major nutritional source of protein for many people that live in West Africa. Annual yields and longevity of grain storage is greatly reduced by feeding damage caused by a complex of insect pests that include the pod sucking bugs, Anoplocnemis curvipes Fabricius (Hemiptera: Coreidae) and Clavigralla tomentosicollis Stål (Hemiptera: Coreidae); as well as phloem-feeding cowpea aphids, Aphis craccivora Koch
(Hemiptera: Aphididae) and flower thrips, Megalurothrips sjostedti Trybom (Thysanoptera: Thripidae). Efforts to control these pests remain a challenge and there is a need to understand the structure and movement of these pest populations in order to facilitate the development of integrated pest management strategies (IPM). Molecular tools have the potential to help facilitate a better understanding of pest populations. Towards this goal, we used 454 pyrosequencing technology to generate 319,126, 176,262, 320,722 and 227,882 raw reads from A. curvipes, A. craccivora, C. tomentosicollis and M. sjostedti, respectively. The reads were de novo assembled into 11,687, 7,647,10,652 and 7,348 transcripts for A. curvipes, A. craccivora, C. tomentosicollis and M. sjostedti, respectively.Functional annotation of the resulting transcripts identified genes putatively involved in insecticide resistance,
pathogen defense and immunity. Additionally, sequences that matched the primary aphid endosymbiont, Buchneraaphidicola, were identified among A. craccivora transcripts. Furthermore, 742, 97, 607 and 180 single nucleotide polymorphisms (SNPs) were respectively predicted among A. curvipes, A. craccivora, C. tomentosicollis and M.sjostedti transcripts, and will likely be valuable tools for future molecular genetic marker development. These results demonstrate that Roche 454-based transcriptome sequencing could be useful for the development of genomic resources for cowpea pest insects in West Africa. |
| format | Journal Article |
| id | CGSpace76659 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | Public Library of Science |
| publisherStr | Public Library of Science |
| record_format | dspace |
| spelling | CGSpace766592025-11-11T10:29:23Z Development of reference transcriptomes for the major field insect pests of cowpea: a toolbox for insect pest management approaches in West Africa Agunbiade, T.A. Sun, W. Coates, B.S. Djouaka, R.F. Tamo, Manuele Ba, Malick N. Binso-Dabire, L. Baoua, I. Olds, B.P. Pittendrigh, Barry Robert pest management cowpeas pest control grain legumes Cowpea is a widely cultivated and major nutritional source of protein for many people that live in West Africa. Annual yields and longevity of grain storage is greatly reduced by feeding damage caused by a complex of insect pests that include the pod sucking bugs, Anoplocnemis curvipes Fabricius (Hemiptera: Coreidae) and Clavigralla tomentosicollis Stål (Hemiptera: Coreidae); as well as phloem-feeding cowpea aphids, Aphis craccivora Koch (Hemiptera: Aphididae) and flower thrips, Megalurothrips sjostedti Trybom (Thysanoptera: Thripidae). Efforts to control these pests remain a challenge and there is a need to understand the structure and movement of these pest populations in order to facilitate the development of integrated pest management strategies (IPM). Molecular tools have the potential to help facilitate a better understanding of pest populations. Towards this goal, we used 454 pyrosequencing technology to generate 319,126, 176,262, 320,722 and 227,882 raw reads from A. curvipes, A. craccivora, C. tomentosicollis and M. sjostedti, respectively. The reads were de novo assembled into 11,687, 7,647,10,652 and 7,348 transcripts for A. curvipes, A. craccivora, C. tomentosicollis and M. sjostedti, respectively.Functional annotation of the resulting transcripts identified genes putatively involved in insecticide resistance, pathogen defense and immunity. Additionally, sequences that matched the primary aphid endosymbiont, Buchneraaphidicola, were identified among A. craccivora transcripts. Furthermore, 742, 97, 607 and 180 single nucleotide polymorphisms (SNPs) were respectively predicted among A. curvipes, A. craccivora, C. tomentosicollis and M.sjostedti transcripts, and will likely be valuable tools for future molecular genetic marker development. These results demonstrate that Roche 454-based transcriptome sequencing could be useful for the development of genomic resources for cowpea pest insects in West Africa. 2013-11 2016-08-30T12:46:13Z 2016-08-30T12:46:13Z Journal Article https://hdl.handle.net/10568/76659 en Open Access application/pdf Public Library of Science Agunbiade, T.A., Sun, W., Coates, B.S., Djouaka, R.F., Tamo, M., Ba, M.N., ... & Pittendrigh, B. R. (2013). Development of reference transcriptomes for the major field insect pests of cowpea: a toolbox for insect pest management approaches in West Africa. PLoS ONE, 8(11), 1-14. |
| spellingShingle | pest management cowpeas pest control grain legumes Agunbiade, T.A. Sun, W. Coates, B.S. Djouaka, R.F. Tamo, Manuele Ba, Malick N. Binso-Dabire, L. Baoua, I. Olds, B.P. Pittendrigh, Barry Robert Development of reference transcriptomes for the major field insect pests of cowpea: a toolbox for insect pest management approaches in West Africa |
| title | Development of reference transcriptomes for the major field insect pests of cowpea: a toolbox for insect pest management approaches in West Africa |
| title_full | Development of reference transcriptomes for the major field insect pests of cowpea: a toolbox for insect pest management approaches in West Africa |
| title_fullStr | Development of reference transcriptomes for the major field insect pests of cowpea: a toolbox for insect pest management approaches in West Africa |
| title_full_unstemmed | Development of reference transcriptomes for the major field insect pests of cowpea: a toolbox for insect pest management approaches in West Africa |
| title_short | Development of reference transcriptomes for the major field insect pests of cowpea: a toolbox for insect pest management approaches in West Africa |
| title_sort | development of reference transcriptomes for the major field insect pests of cowpea a toolbox for insect pest management approaches in west africa |
| topic | pest management cowpeas pest control grain legumes |
| url | https://hdl.handle.net/10568/76659 |
| work_keys_str_mv | AT agunbiadeta developmentofreferencetranscriptomesforthemajorfieldinsectpestsofcowpeaatoolboxforinsectpestmanagementapproachesinwestafrica AT sunw developmentofreferencetranscriptomesforthemajorfieldinsectpestsofcowpeaatoolboxforinsectpestmanagementapproachesinwestafrica AT coatesbs developmentofreferencetranscriptomesforthemajorfieldinsectpestsofcowpeaatoolboxforinsectpestmanagementapproachesinwestafrica AT djouakarf developmentofreferencetranscriptomesforthemajorfieldinsectpestsofcowpeaatoolboxforinsectpestmanagementapproachesinwestafrica AT tamomanuele developmentofreferencetranscriptomesforthemajorfieldinsectpestsofcowpeaatoolboxforinsectpestmanagementapproachesinwestafrica AT bamalickn developmentofreferencetranscriptomesforthemajorfieldinsectpestsofcowpeaatoolboxforinsectpestmanagementapproachesinwestafrica AT binsodabirel developmentofreferencetranscriptomesforthemajorfieldinsectpestsofcowpeaatoolboxforinsectpestmanagementapproachesinwestafrica AT baouai developmentofreferencetranscriptomesforthemajorfieldinsectpestsofcowpeaatoolboxforinsectpestmanagementapproachesinwestafrica AT oldsbp developmentofreferencetranscriptomesforthemajorfieldinsectpestsofcowpeaatoolboxforinsectpestmanagementapproachesinwestafrica AT pittendrighbarryrobert developmentofreferencetranscriptomesforthemajorfieldinsectpestsofcowpeaatoolboxforinsectpestmanagementapproachesinwestafrica |