Microsatellite development for the genus Guibourtia (Fabaceae, Caesalpinioideae) reveals diploid and polyploid species
Premise of the study: Nuclear microsatellites (nSSRs) were designed for Guibourtia tessmannii (Fabaceae, Caesalpinioideae), a highly exploited African timber tree, to study population genetic structure and gene flow. Methods and Results: We developed 16 polymorphic nSSRs from a genomic library tes...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2016
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/76533 |
| _version_ | 1855536721908727808 |
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| author | Tosso, F. Doucet, J.L. Kaymak, E. Dainou, K. Duminil, J. Hardy, Olivier J. |
| author_browse | Dainou, K. Doucet, J.L. Duminil, J. Hardy, Olivier J. Kaymak, E. Tosso, F. |
| author_facet | Tosso, F. Doucet, J.L. Kaymak, E. Dainou, K. Duminil, J. Hardy, Olivier J. |
| author_sort | Tosso, F. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Premise of the study: Nuclear microsatellites (nSSRs) were designed for Guibourtia tessmannii (Fabaceae, Caesalpinioideae), a highly exploited African timber tree, to study population genetic structure and gene flow.
Methods and Results: We developed 16 polymorphic nSSRs from a genomic library tested in three populations of G. tessmannii and two populations of G. coleosperma. These nSSRs display three to 14 alleles per locus (mean 8.94) in G. tessmannii. Cross-amplification tests in nine congeneric species demonstrated that the genus Guibourtia contains diploid and polyploid species. Flow cytometry results combined with nSSR profiles suggest that G. tessmannii is octoploid.
Conclusions: nSSRs revealed that African Guibourtia species include both diploid and polyploid species. These markers will provide information on the mating system, patterns of gene flow, and genetic structure of African Guibourtia species. |
| format | Journal Article |
| id | CGSpace76533 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace765332025-11-12T05:47:07Z Microsatellite development for the genus Guibourtia (Fabaceae, Caesalpinioideae) reveals diploid and polyploid species Tosso, F. Doucet, J.L. Kaymak, E. Dainou, K. Duminil, J. Hardy, Olivier J. fabaceae ploidy cell components microsatellites dna sequence gene flow Premise of the study: Nuclear microsatellites (nSSRs) were designed for Guibourtia tessmannii (Fabaceae, Caesalpinioideae), a highly exploited African timber tree, to study population genetic structure and gene flow. Methods and Results: We developed 16 polymorphic nSSRs from a genomic library tested in three populations of G. tessmannii and two populations of G. coleosperma. These nSSRs display three to 14 alleles per locus (mean 8.94) in G. tessmannii. Cross-amplification tests in nine congeneric species demonstrated that the genus Guibourtia contains diploid and polyploid species. Flow cytometry results combined with nSSR profiles suggest that G. tessmannii is octoploid. Conclusions: nSSRs revealed that African Guibourtia species include both diploid and polyploid species. These markers will provide information on the mating system, patterns of gene flow, and genetic structure of African Guibourtia species. 2016-07 2016-08-22T13:05:26Z 2016-08-22T13:05:26Z Journal Article https://hdl.handle.net/10568/76533 en Open Access application/pdf Wiley Tosso, F.; Doucet, J.L.; Kaymak, E.; Dainou, K.; Duminil, J.; Hardy, O.J. (2016) Microsatellite development for the genus Guibourtia (Fabaceae, Caesalpinioideae) reveals diploid and polyploid species. Applications in Plant Science 4(7):1600029 ISSN: 2168-0450 |
| spellingShingle | fabaceae ploidy cell components microsatellites dna sequence gene flow Tosso, F. Doucet, J.L. Kaymak, E. Dainou, K. Duminil, J. Hardy, Olivier J. Microsatellite development for the genus Guibourtia (Fabaceae, Caesalpinioideae) reveals diploid and polyploid species |
| title | Microsatellite development for the genus Guibourtia (Fabaceae, Caesalpinioideae) reveals diploid and polyploid species |
| title_full | Microsatellite development for the genus Guibourtia (Fabaceae, Caesalpinioideae) reveals diploid and polyploid species |
| title_fullStr | Microsatellite development for the genus Guibourtia (Fabaceae, Caesalpinioideae) reveals diploid and polyploid species |
| title_full_unstemmed | Microsatellite development for the genus Guibourtia (Fabaceae, Caesalpinioideae) reveals diploid and polyploid species |
| title_short | Microsatellite development for the genus Guibourtia (Fabaceae, Caesalpinioideae) reveals diploid and polyploid species |
| title_sort | microsatellite development for the genus guibourtia fabaceae caesalpinioideae reveals diploid and polyploid species |
| topic | fabaceae ploidy cell components microsatellites dna sequence gene flow |
| url | https://hdl.handle.net/10568/76533 |
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