Ploidy level of the banana (Musa spp.) accessions at the germplasm collection centre for the East and Central Africa
Banana germplasm collections serves as a source of useful genes for banana breeding. However, insufficient and/or inaccurate information on the ploidy level of the germplasm renders its utilization in breeding difficult. The objective of this study was to determine and validate the ploidy level of 1...
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Academic Journals
2016
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/76522 |
| _version_ | 1855524185051234304 |
|---|---|
| author | Karamura, D. Tumuhimbise, Robooni Muhangi, S. Nyine, M. Pillay, M. Tendo, R.S. Talengera, D. Namanya, P. Kubiriba, Jerome Karamura, E.B. |
| author_browse | Karamura, D. Karamura, E.B. Kubiriba, Jerome Muhangi, S. Namanya, P. Nyine, M. Pillay, M. Talengera, D. Tendo, R.S. Tumuhimbise, Robooni |
| author_facet | Karamura, D. Tumuhimbise, Robooni Muhangi, S. Nyine, M. Pillay, M. Tendo, R.S. Talengera, D. Namanya, P. Kubiriba, Jerome Karamura, E.B. |
| author_sort | Karamura, D. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Banana germplasm collections serves as a source of useful genes for banana breeding. However, insufficient and/or inaccurate information on the ploidy level of the germplasm renders its utilization in breeding difficult. The objective of this study was to determine and validate the ploidy level of 120 banana accessions in the ex situ germplasm collection centre for the East and Central Africa, located in Mbarara, Uganda. Flow cytometric analysis of the nuclear DNA content was used to determine the ploidy level of the accessions. Results indicate that accessions: Bura, Diana, Kambani-Rungwe, Paji and Pagatau, and Rungwe that were previously classified as diploids are actually triploids, whereas Selangor previously known to be a diploid is a tetraploid. Accessions such as Galeo, Mwitupemba and Ntindi 1 that were previously classified as triploids were found diploids. GT, FHIA 25 and Muzungu Mwekundu that were considered as tetraploids, were found triploids. The information generated will guide correct placement of these accessions in the regional germplasm collection centre for the East and Central Africa and their utilization in banana breeding. |
| format | Journal Article |
| id | CGSpace76522 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Academic Journals |
| publisherStr | Academic Journals |
| record_format | dspace |
| spelling | CGSpace765222025-11-12T05:41:44Z Ploidy level of the banana (Musa spp.) accessions at the germplasm collection centre for the East and Central Africa Karamura, D. Tumuhimbise, Robooni Muhangi, S. Nyine, M. Pillay, M. Tendo, R.S. Talengera, D. Namanya, P. Kubiriba, Jerome Karamura, E.B. bananas germplasm breeding germplasm collections ploidy cell components Banana germplasm collections serves as a source of useful genes for banana breeding. However, insufficient and/or inaccurate information on the ploidy level of the germplasm renders its utilization in breeding difficult. The objective of this study was to determine and validate the ploidy level of 120 banana accessions in the ex situ germplasm collection centre for the East and Central Africa, located in Mbarara, Uganda. Flow cytometric analysis of the nuclear DNA content was used to determine the ploidy level of the accessions. Results indicate that accessions: Bura, Diana, Kambani-Rungwe, Paji and Pagatau, and Rungwe that were previously classified as diploids are actually triploids, whereas Selangor previously known to be a diploid is a tetraploid. Accessions such as Galeo, Mwitupemba and Ntindi 1 that were previously classified as triploids were found diploids. GT, FHIA 25 and Muzungu Mwekundu that were considered as tetraploids, were found triploids. The information generated will guide correct placement of these accessions in the regional germplasm collection centre for the East and Central Africa and their utilization in banana breeding. 2016 2016-08-19T10:12:31Z 2016-08-19T10:12:31Z Journal Article https://hdl.handle.net/10568/76522 en Open Access application/pdf Academic Journals Karamura, D.; Tumuhimbise, R.; Muhangi, S.; Nyine, M.; Pillay, M.; Tendo, R.S.; Talengera, D.; Namanya, P.; Kubiriba, J.; Karamura, E. (2016) Ploidy level of the banana (Musa spp.) accessions at the germplasm collection centre for the East and Central Africa. African Journal of Biotechnology 15(31) p. 1692-1698. ISSN: 1684-5315 |
| spellingShingle | bananas germplasm breeding germplasm collections ploidy cell components Karamura, D. Tumuhimbise, Robooni Muhangi, S. Nyine, M. Pillay, M. Tendo, R.S. Talengera, D. Namanya, P. Kubiriba, Jerome Karamura, E.B. Ploidy level of the banana (Musa spp.) accessions at the germplasm collection centre for the East and Central Africa |
| title | Ploidy level of the banana (Musa spp.) accessions at the germplasm collection centre for the East and Central Africa |
| title_full | Ploidy level of the banana (Musa spp.) accessions at the germplasm collection centre for the East and Central Africa |
| title_fullStr | Ploidy level of the banana (Musa spp.) accessions at the germplasm collection centre for the East and Central Africa |
| title_full_unstemmed | Ploidy level of the banana (Musa spp.) accessions at the germplasm collection centre for the East and Central Africa |
| title_short | Ploidy level of the banana (Musa spp.) accessions at the germplasm collection centre for the East and Central Africa |
| title_sort | ploidy level of the banana musa spp accessions at the germplasm collection centre for the east and central africa |
| topic | bananas germplasm breeding germplasm collections ploidy cell components |
| url | https://hdl.handle.net/10568/76522 |
| work_keys_str_mv | AT karamurad ploidylevelofthebananamusasppaccessionsatthegermplasmcollectioncentrefortheeastandcentralafrica AT tumuhimbiserobooni ploidylevelofthebananamusasppaccessionsatthegermplasmcollectioncentrefortheeastandcentralafrica AT muhangis ploidylevelofthebananamusasppaccessionsatthegermplasmcollectioncentrefortheeastandcentralafrica AT nyinem ploidylevelofthebananamusasppaccessionsatthegermplasmcollectioncentrefortheeastandcentralafrica AT pillaym ploidylevelofthebananamusasppaccessionsatthegermplasmcollectioncentrefortheeastandcentralafrica AT tendors ploidylevelofthebananamusasppaccessionsatthegermplasmcollectioncentrefortheeastandcentralafrica AT talengerad ploidylevelofthebananamusasppaccessionsatthegermplasmcollectioncentrefortheeastandcentralafrica AT namanyap ploidylevelofthebananamusasppaccessionsatthegermplasmcollectioncentrefortheeastandcentralafrica AT kubiribajerome ploidylevelofthebananamusasppaccessionsatthegermplasmcollectioncentrefortheeastandcentralafrica AT karamuraeb ploidylevelofthebananamusasppaccessionsatthegermplasmcollectioncentrefortheeastandcentralafrica |