An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction

Polymerase chain reaction (PCR)-based applications in plant molecular biology and molecular diagnostics for plant pathogens require good quality DNA for reliable and reproducible results. Leaf tissue is often the choice for DNA extraction, but the use of other sources such as tubers, stems, or seeds...

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Main Authors: Sharma, K., Bhattacharjee, Ranjana, Sartie, A.M., Kumar, P. Lava
Format: Journal Article
Language:Inglés
Published: Academic Journals 2013
Subjects:
Online Access:https://hdl.handle.net/10568/76398
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author Sharma, K.
Bhattacharjee, Ranjana
Sartie, A.M.
Kumar, P. Lava
author_browse Bhattacharjee, Ranjana
Kumar, P. Lava
Sartie, A.M.
Sharma, K.
author_facet Sharma, K.
Bhattacharjee, Ranjana
Sartie, A.M.
Kumar, P. Lava
author_sort Sharma, K.
collection Repository of Agricultural Research Outputs (CGSpace)
description Polymerase chain reaction (PCR)-based applications in plant molecular biology and molecular diagnostics for plant pathogens require good quality DNA for reliable and reproducible results. Leaf tissue is often the choice for DNA extraction, but the use of other sources such as tubers, stems, or seeds, is not uncommon. The extraction of DNA from different tissue sources often requires different protocols. In this study, a simple protocol was established for the extraction of DNA from leaves, tubers, stems, seeds and even fungal mycelia. The protocol is simple and suitable for high-throughput DNA extraction using automated tissue grinders. It yielded large quantities of DNA (0.4 ?g to 2 mg DNA from 100 mg tissue) of high quality from seeds of maize, soybean, and cowpea, tubers of yam, tuberous roots of cassava, and leaf tissues of banana and plantain, yam, cassava, maize, okra, mango, and other species. DNA was successfully used for the detection of fungal and viral pathogens and the genotyping of yam and cassava by PCR.
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spelling CGSpace763982024-04-25T06:01:38Z An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction Sharma, K. Bhattacharjee, Ranjana Sartie, A.M. Kumar, P. Lava plant tissues pathogens genotypes dna Polymerase chain reaction (PCR)-based applications in plant molecular biology and molecular diagnostics for plant pathogens require good quality DNA for reliable and reproducible results. Leaf tissue is often the choice for DNA extraction, but the use of other sources such as tubers, stems, or seeds, is not uncommon. The extraction of DNA from different tissue sources often requires different protocols. In this study, a simple protocol was established for the extraction of DNA from leaves, tubers, stems, seeds and even fungal mycelia. The protocol is simple and suitable for high-throughput DNA extraction using automated tissue grinders. It yielded large quantities of DNA (0.4 ?g to 2 mg DNA from 100 mg tissue) of high quality from seeds of maize, soybean, and cowpea, tubers of yam, tuberous roots of cassava, and leaf tissues of banana and plantain, yam, cassava, maize, okra, mango, and other species. DNA was successfully used for the detection of fungal and viral pathogens and the genotyping of yam and cassava by PCR. 2013-04 2016-08-12T06:27:15Z 2016-08-12T06:27:15Z Journal Article https://hdl.handle.net/10568/76398 en Limited Access Academic Journals Sharma, K., Bhattacharjee, R., Sartie, A.M. & Kumar, P.L. (2016). An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction. African Journal of Biotechnology, 12(15).
spellingShingle plant tissues
pathogens
genotypes
dna
Sharma, K.
Bhattacharjee, Ranjana
Sartie, A.M.
Kumar, P. Lava
An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction
title An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction
title_full An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction
title_fullStr An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction
title_full_unstemmed An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction
title_short An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction
title_sort improved method of dna extraction from plants for pathogen detection and genotyping by polymerase chain reaction
topic plant tissues
pathogens
genotypes
dna
url https://hdl.handle.net/10568/76398
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