An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction
Polymerase chain reaction (PCR)-based applications in plant molecular biology and molecular diagnostics for plant pathogens require good quality DNA for reliable and reproducible results. Leaf tissue is often the choice for DNA extraction, but the use of other sources such as tubers, stems, or seeds...
| Main Authors: | , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Academic Journals
2013
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/76398 |
| _version_ | 1855536879707881472 |
|---|---|
| author | Sharma, K. Bhattacharjee, Ranjana Sartie, A.M. Kumar, P. Lava |
| author_browse | Bhattacharjee, Ranjana Kumar, P. Lava Sartie, A.M. Sharma, K. |
| author_facet | Sharma, K. Bhattacharjee, Ranjana Sartie, A.M. Kumar, P. Lava |
| author_sort | Sharma, K. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Polymerase chain reaction (PCR)-based applications in plant molecular biology and molecular diagnostics for plant pathogens require good quality DNA for reliable and reproducible results. Leaf tissue is often the choice for DNA extraction, but the use of other sources such as tubers, stems, or seeds, is not uncommon. The extraction of DNA from different tissue sources often requires different protocols. In this study, a simple protocol was established for the extraction of DNA from leaves, tubers, stems, seeds and even fungal mycelia. The protocol is simple and suitable for high-throughput DNA extraction using automated tissue grinders. It yielded large quantities of DNA (0.4 ?g to 2 mg DNA from 100 mg tissue) of high quality from seeds of maize, soybean, and cowpea, tubers of yam, tuberous roots of cassava, and leaf tissues of banana and plantain, yam, cassava, maize, okra, mango, and other species. DNA was successfully used for the detection of fungal and viral pathogens and the genotyping of yam and cassava by PCR. |
| format | Journal Article |
| id | CGSpace76398 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | Academic Journals |
| publisherStr | Academic Journals |
| record_format | dspace |
| spelling | CGSpace763982024-04-25T06:01:38Z An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction Sharma, K. Bhattacharjee, Ranjana Sartie, A.M. Kumar, P. Lava plant tissues pathogens genotypes dna Polymerase chain reaction (PCR)-based applications in plant molecular biology and molecular diagnostics for plant pathogens require good quality DNA for reliable and reproducible results. Leaf tissue is often the choice for DNA extraction, but the use of other sources such as tubers, stems, or seeds, is not uncommon. The extraction of DNA from different tissue sources often requires different protocols. In this study, a simple protocol was established for the extraction of DNA from leaves, tubers, stems, seeds and even fungal mycelia. The protocol is simple and suitable for high-throughput DNA extraction using automated tissue grinders. It yielded large quantities of DNA (0.4 ?g to 2 mg DNA from 100 mg tissue) of high quality from seeds of maize, soybean, and cowpea, tubers of yam, tuberous roots of cassava, and leaf tissues of banana and plantain, yam, cassava, maize, okra, mango, and other species. DNA was successfully used for the detection of fungal and viral pathogens and the genotyping of yam and cassava by PCR. 2013-04 2016-08-12T06:27:15Z 2016-08-12T06:27:15Z Journal Article https://hdl.handle.net/10568/76398 en Limited Access Academic Journals Sharma, K., Bhattacharjee, R., Sartie, A.M. & Kumar, P.L. (2016). An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction. African Journal of Biotechnology, 12(15). |
| spellingShingle | plant tissues pathogens genotypes dna Sharma, K. Bhattacharjee, Ranjana Sartie, A.M. Kumar, P. Lava An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction |
| title | An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction |
| title_full | An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction |
| title_fullStr | An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction |
| title_full_unstemmed | An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction |
| title_short | An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction |
| title_sort | improved method of dna extraction from plants for pathogen detection and genotyping by polymerase chain reaction |
| topic | plant tissues pathogens genotypes dna |
| url | https://hdl.handle.net/10568/76398 |
| work_keys_str_mv | AT sharmak animprovedmethodofdnaextractionfromplantsforpathogendetectionandgenotypingbypolymerasechainreaction AT bhattacharjeeranjana animprovedmethodofdnaextractionfromplantsforpathogendetectionandgenotypingbypolymerasechainreaction AT sartieam animprovedmethodofdnaextractionfromplantsforpathogendetectionandgenotypingbypolymerasechainreaction AT kumarplava animprovedmethodofdnaextractionfromplantsforpathogendetectionandgenotypingbypolymerasechainreaction AT sharmak improvedmethodofdnaextractionfromplantsforpathogendetectionandgenotypingbypolymerasechainreaction AT bhattacharjeeranjana improvedmethodofdnaextractionfromplantsforpathogendetectionandgenotypingbypolymerasechainreaction AT sartieam improvedmethodofdnaextractionfromplantsforpathogendetectionandgenotypingbypolymerasechainreaction AT kumarplava improvedmethodofdnaextractionfromplantsforpathogendetectionandgenotypingbypolymerasechainreaction |