Interspecific crossing between yam species (Dioscorea rotundata and Dioscorea bulbifera) through in vitro ovule culture
In the present study, in vitro ovule culture technique was used to obtain interspecific cross combination of Dioscorea rotundata ufenyi and Dioscorea bulbifera wild. Ten days after pollination, ovules were excised and cultured onto 1/2 strength Murashige and Skoog (MS) medium (Basal salt mixture + V...
| Autores principales: | , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Scientific Research Publishing, Inc.
2016
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/76358 |
| _version_ | 1855514031195947008 |
|---|---|
| author | Saini, H. Kashihara, Y. López Montes, Antonio José Asiedu, Robert |
| author_browse | Asiedu, Robert Kashihara, Y. López Montes, Antonio José Saini, H. |
| author_facet | Saini, H. Kashihara, Y. López Montes, Antonio José Asiedu, Robert |
| author_sort | Saini, H. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | In the present study, in vitro ovule culture technique was used to obtain interspecific cross combination of Dioscorea rotundata ufenyi and Dioscorea bulbifera wild. Ten days after pollination, ovules were excised and cultured onto 1/2 strength Murashige and Skoog (MS) medium (Basal salt mixture + Vitamins) supplemented with 6% sucrose, 0.7% agar and plant growth hormones such as GA3, BAP, Picrolam and TDZ. Cultured ovules were transferred on 1/2 MS medium with 3% sucrose and 0.7% agar after three weeks. 40 days after pollination, germination was observed from 7 months cultured ovule between D. rotundata ufenyi x D. bulbifera wild. Hybridity of the regenerated plant was checked by flow cytometric method. A close relation was observed between the fluorescence intensity of the obtained progeny with one of the parents’ fluorescence. The observed progeny can be closely correlated with an apomictic tissue from an ovule parent of D. rotundata ufenyi. Plantlets derived from ovule culture were proliferated through in vitro shoot multiplication with hormonal concentration (0.5 mg/l BAP) supplemented with 1/2 strength MS medium. Obtained ovule culture derived in vitro plantlets were successfully hardened, acclimatized and transferred to the field, where they survived and grew normally. In plant breeding, interspecific crossing is very important technique, enabling the time needed to produce homozygous lines to be shortened as compared to the conventional plant breeding techniques. |
| format | Journal Article |
| id | CGSpace76358 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Scientific Research Publishing, Inc. |
| publisherStr | Scientific Research Publishing, Inc. |
| record_format | dspace |
| spelling | CGSpace763582025-11-11T10:01:26Z Interspecific crossing between yam species (Dioscorea rotundata and Dioscorea bulbifera) through in vitro ovule culture Saini, H. Kashihara, Y. López Montes, Antonio José Asiedu, Robert in vitro dioscorea rotundata dioscorea bulbifera In the present study, in vitro ovule culture technique was used to obtain interspecific cross combination of Dioscorea rotundata ufenyi and Dioscorea bulbifera wild. Ten days after pollination, ovules were excised and cultured onto 1/2 strength Murashige and Skoog (MS) medium (Basal salt mixture + Vitamins) supplemented with 6% sucrose, 0.7% agar and plant growth hormones such as GA3, BAP, Picrolam and TDZ. Cultured ovules were transferred on 1/2 MS medium with 3% sucrose and 0.7% agar after three weeks. 40 days after pollination, germination was observed from 7 months cultured ovule between D. rotundata ufenyi x D. bulbifera wild. Hybridity of the regenerated plant was checked by flow cytometric method. A close relation was observed between the fluorescence intensity of the obtained progeny with one of the parents’ fluorescence. The observed progeny can be closely correlated with an apomictic tissue from an ovule parent of D. rotundata ufenyi. Plantlets derived from ovule culture were proliferated through in vitro shoot multiplication with hormonal concentration (0.5 mg/l BAP) supplemented with 1/2 strength MS medium. Obtained ovule culture derived in vitro plantlets were successfully hardened, acclimatized and transferred to the field, where they survived and grew normally. In plant breeding, interspecific crossing is very important technique, enabling the time needed to produce homozygous lines to be shortened as compared to the conventional plant breeding techniques. 2016 2016-08-09T12:28:34Z 2016-08-09T12:28:34Z Journal Article https://hdl.handle.net/10568/76358 en Open Access application/pdf Scientific Research Publishing, Inc. Saini, H., Kashihara, Y., Lopez-Montes, A. & Asiedu, R. (2016). Interspecific Crossing between Yam Species (Dioscorea rotundata and Dioscorea bulbifera) through in Vitro Ovule Culture. American Journal of Plant Sciences, 7(8), 1268-1274 |
| spellingShingle | in vitro dioscorea rotundata dioscorea bulbifera Saini, H. Kashihara, Y. López Montes, Antonio José Asiedu, Robert Interspecific crossing between yam species (Dioscorea rotundata and Dioscorea bulbifera) through in vitro ovule culture |
| title | Interspecific crossing between yam species (Dioscorea rotundata and Dioscorea bulbifera) through in vitro ovule culture |
| title_full | Interspecific crossing between yam species (Dioscorea rotundata and Dioscorea bulbifera) through in vitro ovule culture |
| title_fullStr | Interspecific crossing between yam species (Dioscorea rotundata and Dioscorea bulbifera) through in vitro ovule culture |
| title_full_unstemmed | Interspecific crossing between yam species (Dioscorea rotundata and Dioscorea bulbifera) through in vitro ovule culture |
| title_short | Interspecific crossing between yam species (Dioscorea rotundata and Dioscorea bulbifera) through in vitro ovule culture |
| title_sort | interspecific crossing between yam species dioscorea rotundata and dioscorea bulbifera through in vitro ovule culture |
| topic | in vitro dioscorea rotundata dioscorea bulbifera |
| url | https://hdl.handle.net/10568/76358 |
| work_keys_str_mv | AT sainih interspecificcrossingbetweenyamspeciesdioscorearotundataanddioscoreabulbiferathroughinvitroovuleculture AT kashiharay interspecificcrossingbetweenyamspeciesdioscorearotundataanddioscoreabulbiferathroughinvitroovuleculture AT lopezmontesantoniojose interspecificcrossingbetweenyamspeciesdioscorearotundataanddioscoreabulbiferathroughinvitroovuleculture AT asiedurobert interspecificcrossingbetweenyamspeciesdioscorearotundataanddioscoreabulbiferathroughinvitroovuleculture |