Molecular differentiation of the African yellow fever vector Aedes bromeliae (Diptera: Culicidae) from its sympatric nonvector sister species, Aedes lilii
Introduction Yellow fever continues to be a problem in sub-Saharan Africa with repeated epidemics occurring. The mosquito Aedes bromeliae is a major vector of yellow fever, but it cannot be readily differentiated from its non-vector zoophilic sister species Ae. lilii using morphological characters....
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Public Library of Science
2015
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| Acceso en línea: | https://hdl.handle.net/10568/76334 |
| _version_ | 1855516755974160384 |
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| author | Bennett, K.L. Linton, Y. Shija, F. Kaddumukasa, M. Djouaka, R.F. Misinzo, G. Lutwama, J. Huang, Y. Mitchell, L.B. Richards, Meryl B. Tossou, E. Walton, C. |
| author_browse | Bennett, K.L. Djouaka, R.F. Huang, Y. Kaddumukasa, M. Linton, Y. Lutwama, J. Misinzo, G. Mitchell, L.B. Richards, Meryl B. Shija, F. Tossou, E. Walton, C. |
| author_facet | Bennett, K.L. Linton, Y. Shija, F. Kaddumukasa, M. Djouaka, R.F. Misinzo, G. Lutwama, J. Huang, Y. Mitchell, L.B. Richards, Meryl B. Tossou, E. Walton, C. |
| author_sort | Bennett, K.L. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Introduction
Yellow fever continues to be a problem in sub-Saharan Africa with repeated epidemics occurring. The mosquito Aedes bromeliae is a major vector of yellow fever, but it cannot be readily differentiated from its non-vector zoophilic sister species Ae. lilii using morphological characters. Genetic differences have been reported between anthropophilic Ae. bromeliae and zoophilic Ae. lilii and between forest and domestic populations. However, due to the application of different molecular markers and non-overlapping populations employed in previous studies, interpretation of species delimitation is unclear.
Methodology/Principle Findings
DNA sequences were generated from specimens of Ae. simpsoni s.l. from the Republic of Benin, Tanzania and Uganda for two nuclear genes apolipophorin 2 (apoLp2) and cytochrome p450 (CYPJ92), the ribosomal internal transcribed spacer region (ITS) and the mitochondrial cytochrome c oxidase (COI) barcoding region. Nuclear genes apoLp2 and CYPJ92 were unable to differentiate between species Ae. bromeliae and Ae. lilii due to ancestral lineage sorting, while ITS sequence data provided clear topological separation on a phylogeny. The standard COI barcoding region was shown to be subject to species introgression and unable to clearly distinguish the two taxa. Here we present a reliable direct PCR-based method for differentiation of the vector species Ae. bromeliae from itsisomorphic, sympatric and non-biomedically important sister taxon, Ae. lilii, based on the ITS region. Using molecular species verification, we describe novel immature habitats for Ae. lilii and report both sympatric and allopatric populations. Whereas only Ae. lilii is found in the Republic of Benin and only Ae. bromeliae in Tanzania, both species are sympatric in Uganda.
Conclusions/Significance
Our accurate identification method will allow informed distribution and detailed ecological studies that will facilitate assessment of arboviral disease risk and development of future targeted vector control. |
| format | Journal Article |
| id | CGSpace76334 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Public Library of Science |
| publisherStr | Public Library of Science |
| record_format | dspace |
| spelling | CGSpace763342025-11-11T10:05:17Z Molecular differentiation of the African yellow fever vector Aedes bromeliae (Diptera: Culicidae) from its sympatric nonvector sister species, Aedes lilii Bennett, K.L. Linton, Y. Shija, F. Kaddumukasa, M. Djouaka, R.F. Misinzo, G. Lutwama, J. Huang, Y. Mitchell, L.B. Richards, Meryl B. Tossou, E. Walton, C. dna ribosomal phylogeny vector infectious diseases Introduction Yellow fever continues to be a problem in sub-Saharan Africa with repeated epidemics occurring. The mosquito Aedes bromeliae is a major vector of yellow fever, but it cannot be readily differentiated from its non-vector zoophilic sister species Ae. lilii using morphological characters. Genetic differences have been reported between anthropophilic Ae. bromeliae and zoophilic Ae. lilii and between forest and domestic populations. However, due to the application of different molecular markers and non-overlapping populations employed in previous studies, interpretation of species delimitation is unclear. Methodology/Principle Findings DNA sequences were generated from specimens of Ae. simpsoni s.l. from the Republic of Benin, Tanzania and Uganda for two nuclear genes apolipophorin 2 (apoLp2) and cytochrome p450 (CYPJ92), the ribosomal internal transcribed spacer region (ITS) and the mitochondrial cytochrome c oxidase (COI) barcoding region. Nuclear genes apoLp2 and CYPJ92 were unable to differentiate between species Ae. bromeliae and Ae. lilii due to ancestral lineage sorting, while ITS sequence data provided clear topological separation on a phylogeny. The standard COI barcoding region was shown to be subject to species introgression and unable to clearly distinguish the two taxa. Here we present a reliable direct PCR-based method for differentiation of the vector species Ae. bromeliae from itsisomorphic, sympatric and non-biomedically important sister taxon, Ae. lilii, based on the ITS region. Using molecular species verification, we describe novel immature habitats for Ae. lilii and report both sympatric and allopatric populations. Whereas only Ae. lilii is found in the Republic of Benin and only Ae. bromeliae in Tanzania, both species are sympatric in Uganda. Conclusions/Significance Our accurate identification method will allow informed distribution and detailed ecological studies that will facilitate assessment of arboviral disease risk and development of future targeted vector control. 2015-12-07 2016-08-04T07:58:08Z 2016-08-04T07:58:08Z Journal Article https://hdl.handle.net/10568/76334 en Open Access application/pdf Public Library of Science Bennett, K.L., Linton, Y.M., Shija, F., Kaddumukasa, M., Djouaka, R., Misinzo, G., ... & Tossou, E. (2015). Molecular differentiation of the African yellow fever vector Aedes bromeliae (Diptera: Culicidae) from its sympatric non-vector sister species, Aedes lilii. PLoS Negl Trop Dis, 9(12), e0004250. |
| spellingShingle | dna ribosomal phylogeny vector infectious diseases Bennett, K.L. Linton, Y. Shija, F. Kaddumukasa, M. Djouaka, R.F. Misinzo, G. Lutwama, J. Huang, Y. Mitchell, L.B. Richards, Meryl B. Tossou, E. Walton, C. Molecular differentiation of the African yellow fever vector Aedes bromeliae (Diptera: Culicidae) from its sympatric nonvector sister species, Aedes lilii |
| title | Molecular differentiation of the African yellow fever vector Aedes bromeliae (Diptera: Culicidae) from its sympatric nonvector sister species, Aedes lilii |
| title_full | Molecular differentiation of the African yellow fever vector Aedes bromeliae (Diptera: Culicidae) from its sympatric nonvector sister species, Aedes lilii |
| title_fullStr | Molecular differentiation of the African yellow fever vector Aedes bromeliae (Diptera: Culicidae) from its sympatric nonvector sister species, Aedes lilii |
| title_full_unstemmed | Molecular differentiation of the African yellow fever vector Aedes bromeliae (Diptera: Culicidae) from its sympatric nonvector sister species, Aedes lilii |
| title_short | Molecular differentiation of the African yellow fever vector Aedes bromeliae (Diptera: Culicidae) from its sympatric nonvector sister species, Aedes lilii |
| title_sort | molecular differentiation of the african yellow fever vector aedes bromeliae diptera culicidae from its sympatric nonvector sister species aedes lilii |
| topic | dna ribosomal phylogeny vector infectious diseases |
| url | https://hdl.handle.net/10568/76334 |
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