A parthenogenesis gene candidate and evidence for segmental allopolyploidy in apomictic brachiaria decumbens
Apomixis, asexual reproduction through seed, enables breeders to identify and faithfully propagate superior heterozygous genotypes by seed without the disadvantages of vegetative propagation or the expense and complexity of hybrid seed production. The availability of new tools such as genotyping by...
| Autores principales: | , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Oxford University Press
2016
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/76280 |
| _version_ | 1855532601255657472 |
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| author | Worthington, Margaret Heffelfinger, Christopher Bernal, Diana Quintero, Constanza Zapata, Yeny Patricia Pérez, Juan Guillermo Vega, José de Miles, John W. Dellaporta, Stephen L. Tohme, Joseph M. |
| author_browse | Bernal, Diana Dellaporta, Stephen L. Heffelfinger, Christopher Miles, John W. Pérez, Juan Guillermo Quintero, Constanza Tohme, Joseph M. Vega, José de Worthington, Margaret Zapata, Yeny Patricia |
| author_facet | Worthington, Margaret Heffelfinger, Christopher Bernal, Diana Quintero, Constanza Zapata, Yeny Patricia Pérez, Juan Guillermo Vega, José de Miles, John W. Dellaporta, Stephen L. Tohme, Joseph M. |
| author_sort | Worthington, Margaret |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Apomixis, asexual reproduction through seed, enables breeders to identify and faithfully propagate superior heterozygous genotypes by seed without the disadvantages of vegetative propagation or the expense and complexity of hybrid seed production. The availability of new tools such as genotyping by sequencing and bioinformatics pipelines for species lacking reference genomes now makes the construction of dense maps possible in apomictic species, despite complications including polyploidy, multisomic inheritance, self-incompatibility, and high levels of heterozygosity. In this study, we developed saturated linkage maps for the maternal and paternal genomes of an interspecific Brachiaria ruziziensis (R. Germ. and C. M. Evrard) × B. decumbens Stapf. F1 mapping population in order to identify markers linked to apomixis. High-resolution molecular karyotyping and comparative genomics with Setaria italica (L.) P. Beauv provided conclusive evidence for segmental allopolyploidy in B. decumbens, with strong preferential pairing of homologs across the genome and multisomic segregation relatively more common in chromosome 8. The apospory-specific genomic region (ASGR) was mapped to a region of reduced recombination on B. decumbens chromosome 5. The Pennisetum squamulatum (L.) R.Br. PsASGR-BABY BOOM-like (psASGR–BBML)-specific primer pair p779/p780 was in perfect linkage with the ASGR in the F1 mapping population and diagnostic for reproductive mode in a diversity panel of known sexual and apomict Brachiaria (Trin.) Griseb. and P. maximum Jacq. germplasm accessions and cultivars. These findings indicate that ASGR–BBML gene sequences are highly conserved across the Paniceae and add further support for the postulation of the ASGR–BBML as candidate genes for the apomictic function of parthenogenesis. |
| format | Journal Article |
| id | CGSpace76280 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace762802025-03-13T09:44:45Z A parthenogenesis gene candidate and evidence for segmental allopolyploidy in apomictic brachiaria decumbens Worthington, Margaret Heffelfinger, Christopher Bernal, Diana Quintero, Constanza Zapata, Yeny Patricia Pérez, Juan Guillermo Vega, José de Miles, John W. Dellaporta, Stephen L. Tohme, Joseph M. feed crops poliploidy genetic markers genetic maps hybridization restriction fragment apomixis genotypes brachiaria decumbens plant breeding poliploidia marcadores genéticos mapas genéticos hibridación genotipos Apomixis, asexual reproduction through seed, enables breeders to identify and faithfully propagate superior heterozygous genotypes by seed without the disadvantages of vegetative propagation or the expense and complexity of hybrid seed production. The availability of new tools such as genotyping by sequencing and bioinformatics pipelines for species lacking reference genomes now makes the construction of dense maps possible in apomictic species, despite complications including polyploidy, multisomic inheritance, self-incompatibility, and high levels of heterozygosity. In this study, we developed saturated linkage maps for the maternal and paternal genomes of an interspecific Brachiaria ruziziensis (R. Germ. and C. M. Evrard) × B. decumbens Stapf. F1 mapping population in order to identify markers linked to apomixis. High-resolution molecular karyotyping and comparative genomics with Setaria italica (L.) P. Beauv provided conclusive evidence for segmental allopolyploidy in B. decumbens, with strong preferential pairing of homologs across the genome and multisomic segregation relatively more common in chromosome 8. The apospory-specific genomic region (ASGR) was mapped to a region of reduced recombination on B. decumbens chromosome 5. The Pennisetum squamulatum (L.) R.Br. PsASGR-BABY BOOM-like (psASGR–BBML)-specific primer pair p779/p780 was in perfect linkage with the ASGR in the F1 mapping population and diagnostic for reproductive mode in a diversity panel of known sexual and apomict Brachiaria (Trin.) Griseb. and P. maximum Jacq. germplasm accessions and cultivars. These findings indicate that ASGR–BBML gene sequences are highly conserved across the Paniceae and add further support for the postulation of the ASGR–BBML as candidate genes for the apomictic function of parthenogenesis. 2016-07-01 2016-07-26T22:10:19Z 2016-07-26T22:10:19Z Journal Article https://hdl.handle.net/10568/76280 en Open Access Oxford University Press Worthington, Margaret; Heffelfinger, Christopher; Bernal, Diana; Quintero, Constanza; Zapata, Yeny Patricia; Perez, Juan Guillermo; De Vega, Jose; Miles, John; Dellaporta, Stephen; Tohme, Joseph M.. 2016. A parthenogenesis gene candidate and evidence for segmental allopolyploidy in apomictic brachiaria decumbens. Genetics 203 (3): 1117-1132. |
| spellingShingle | feed crops poliploidy genetic markers genetic maps hybridization restriction fragment apomixis genotypes brachiaria decumbens plant breeding poliploidia marcadores genéticos mapas genéticos hibridación genotipos Worthington, Margaret Heffelfinger, Christopher Bernal, Diana Quintero, Constanza Zapata, Yeny Patricia Pérez, Juan Guillermo Vega, José de Miles, John W. Dellaporta, Stephen L. Tohme, Joseph M. A parthenogenesis gene candidate and evidence for segmental allopolyploidy in apomictic brachiaria decumbens |
| title | A parthenogenesis gene candidate and evidence for segmental allopolyploidy in apomictic brachiaria decumbens |
| title_full | A parthenogenesis gene candidate and evidence for segmental allopolyploidy in apomictic brachiaria decumbens |
| title_fullStr | A parthenogenesis gene candidate and evidence for segmental allopolyploidy in apomictic brachiaria decumbens |
| title_full_unstemmed | A parthenogenesis gene candidate and evidence for segmental allopolyploidy in apomictic brachiaria decumbens |
| title_short | A parthenogenesis gene candidate and evidence for segmental allopolyploidy in apomictic brachiaria decumbens |
| title_sort | parthenogenesis gene candidate and evidence for segmental allopolyploidy in apomictic brachiaria decumbens |
| topic | feed crops poliploidy genetic markers genetic maps hybridization restriction fragment apomixis genotypes brachiaria decumbens plant breeding poliploidia marcadores genéticos mapas genéticos hibridación genotipos |
| url | https://hdl.handle.net/10568/76280 |
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