Capsular polysaccharide from Mycoplasma mycoides subsp.mycoides shows potential for protection against Contagious Bovine Pleuropneumonia
Contagious Bovine Pleuropneumonia (CBPP) is a severe respiratory disease caused byMycoplasma mycoides subsp. mycoides (Mmm) which is widespread in Africa. The capsule polysaccharide (CPS) of Mmm is one of the few identified virulence determinants. In a previous study, immunization of mice against CP...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Elsevier
2016
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| Online Access: | https://hdl.handle.net/10568/76131 |
| _version_ | 1855518856284471296 |
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| author | Mwirigi, M. Nkando, I. Olum, M. Attah-Poku, S. Ochanda, H. Berberov, E. Potter, A. Gerdts, V. Pérez Casal, J. Wesonga, H.O. Soi, R. Naessens, Jan |
| author_browse | Attah-Poku, S. Berberov, E. Gerdts, V. Mwirigi, M. Naessens, Jan Nkando, I. Ochanda, H. Olum, M. Potter, A. Pérez Casal, J. Soi, R. Wesonga, H.O. |
| author_facet | Mwirigi, M. Nkando, I. Olum, M. Attah-Poku, S. Ochanda, H. Berberov, E. Potter, A. Gerdts, V. Pérez Casal, J. Wesonga, H.O. Soi, R. Naessens, Jan |
| author_sort | Mwirigi, M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Contagious Bovine Pleuropneumonia (CBPP) is a severe respiratory disease caused byMycoplasma mycoides subsp. mycoides (Mmm) which is widespread in Africa. The capsule polysaccharide (CPS) of Mmm is one of the few identified virulence determinants. In a previous study, immunization of mice against CPS generated antibodies, but they were not able to prevent multiplication of Mmm in this model animal. However, mice cannot be considered as a suitable animal model, as Mmm does not induce pathology in this species. Our aim was to induce antibody responses to CPS in cattle, and challenge them when they had specific CPS antibody titres similar or higher than those from cattle vaccinated with the live vaccine. The CPS was linked to the carrier protein ovalbumin via a carbodiimide-mediated condensation with 1-ethyl-3(3-imethylaminopropyl) carbodiimide (EDC). Ten animals were immunized twice and challenged three weeks after the booster inoculation, and compared to a group of challenged non-immunized cattle. When administered subcutaneously to adult cattle, the vaccine elicited CPS-specific antibody responses with the same or a higher titre than animals vaccinated with the live vaccine. Pathology in the group of immunized animals was significantly reduced (57%) after challenge with Mmm strain Afadé compared to the non-immunized group, a figure in the range of the protection provided by the live vaccine. |
| format | Journal Article |
| id | CGSpace76131 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace761312024-05-01T08:15:54Z Capsular polysaccharide from Mycoplasma mycoides subsp.mycoides shows potential for protection against Contagious Bovine Pleuropneumonia Mwirigi, M. Nkando, I. Olum, M. Attah-Poku, S. Ochanda, H. Berberov, E. Potter, A. Gerdts, V. Pérez Casal, J. Wesonga, H.O. Soi, R. Naessens, Jan animal diseases disease control immunology Contagious Bovine Pleuropneumonia (CBPP) is a severe respiratory disease caused byMycoplasma mycoides subsp. mycoides (Mmm) which is widespread in Africa. The capsule polysaccharide (CPS) of Mmm is one of the few identified virulence determinants. In a previous study, immunization of mice against CPS generated antibodies, but they were not able to prevent multiplication of Mmm in this model animal. However, mice cannot be considered as a suitable animal model, as Mmm does not induce pathology in this species. Our aim was to induce antibody responses to CPS in cattle, and challenge them when they had specific CPS antibody titres similar or higher than those from cattle vaccinated with the live vaccine. The CPS was linked to the carrier protein ovalbumin via a carbodiimide-mediated condensation with 1-ethyl-3(3-imethylaminopropyl) carbodiimide (EDC). Ten animals were immunized twice and challenged three weeks after the booster inoculation, and compared to a group of challenged non-immunized cattle. When administered subcutaneously to adult cattle, the vaccine elicited CPS-specific antibody responses with the same or a higher titre than animals vaccinated with the live vaccine. Pathology in the group of immunized animals was significantly reduced (57%) after challenge with Mmm strain Afadé compared to the non-immunized group, a figure in the range of the protection provided by the live vaccine. 2016-10 2016-07-12T06:06:39Z 2016-07-12T06:06:39Z Journal Article https://hdl.handle.net/10568/76131 en Limited Access Elsevier Mwirigi, M., Nkando, I., Olum, M., Attah-Poku, S., Ochanda, H., Berberov, E., Potter, A., Gerdts, V., Perez-Casal, J., Wesonga, H., Soi, R. and Naessens, J. 2016. Capsular polysaccharide from Mycoplasma mycoides subsp.mycoides shows potential for protection against Contagious Bovine Pleuropneumonia. Veterinary Immunology and Immunopathology 178:64–69. |
| spellingShingle | animal diseases disease control immunology Mwirigi, M. Nkando, I. Olum, M. Attah-Poku, S. Ochanda, H. Berberov, E. Potter, A. Gerdts, V. Pérez Casal, J. Wesonga, H.O. Soi, R. Naessens, Jan Capsular polysaccharide from Mycoplasma mycoides subsp.mycoides shows potential for protection against Contagious Bovine Pleuropneumonia |
| title | Capsular polysaccharide from Mycoplasma mycoides subsp.mycoides shows potential for protection against Contagious Bovine Pleuropneumonia |
| title_full | Capsular polysaccharide from Mycoplasma mycoides subsp.mycoides shows potential for protection against Contagious Bovine Pleuropneumonia |
| title_fullStr | Capsular polysaccharide from Mycoplasma mycoides subsp.mycoides shows potential for protection against Contagious Bovine Pleuropneumonia |
| title_full_unstemmed | Capsular polysaccharide from Mycoplasma mycoides subsp.mycoides shows potential for protection against Contagious Bovine Pleuropneumonia |
| title_short | Capsular polysaccharide from Mycoplasma mycoides subsp.mycoides shows potential for protection against Contagious Bovine Pleuropneumonia |
| title_sort | capsular polysaccharide from mycoplasma mycoides subsp mycoides shows potential for protection against contagious bovine pleuropneumonia |
| topic | animal diseases disease control immunology |
| url | https://hdl.handle.net/10568/76131 |
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