Pathogenderived resistance using a viral nucleocapsid gene confers only partial nondurable protection in peanut against peanut bud necrosis virus
Genetic engineering of peanut (Arachis hypogaea L.) using the gene encoding for the nucleocapsid protein (N gene) of peanut bud necrosis virus (PBNV; genus Tospovirus, family Bunyaviridae) was used to impart resistance to bud necrosis disease in peanut (PBND), a disease for which no durable resistan...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2013
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/76031 |
| _version_ | 1855529969325703168 |
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| author | Rao, S.C. Bhatnagar-Mathur, P. Kumar, P. Lava Reddy, S. Sharma, K. |
| author_browse | Bhatnagar-Mathur, P. Kumar, P. Lava Rao, S.C. Reddy, S. Sharma, K. |
| author_facet | Rao, S.C. Bhatnagar-Mathur, P. Kumar, P. Lava Reddy, S. Sharma, K. |
| author_sort | Rao, S.C. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Genetic engineering of peanut (Arachis hypogaea L.) using the gene encoding for the nucleocapsid protein (N gene) of peanut bud necrosis virus (PBNV; genus Tospovirus, family Bunyaviridae) was used to impart resistance to bud necrosis disease in peanut (PBND), a disease for which no durable resistance is available in the existing germplasm. Over 200 transgenic lines of peanut var. JL 24 were developed for which integration and expression of the transgenes was confirmed by PCR, Southern hybridization, RT-PCR and western blot analysis. The T1 and T2 generation transgenic plants were assayed through virus challenge in the greenhouse by using mechanical sap inoculation at 1:100 and 1:50 dilutions of PBNV, and they showed varying levels of disease incidence and intensity. Greenhouse and field evaluation with T2 generation plants indicated somewhat superior performance of the three transgenic events that showed considerable reduction in disease incidence. However, only one of these events showed over 75 % reduction in disease incidence when compared to the untransformed control, indicating partial and non-durable resistance to PBND using the viral N-gene. |
| format | Journal Article |
| id | CGSpace76031 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace760312023-12-08T19:36:04Z Pathogenderived resistance using a viral nucleocapsid gene confers only partial nondurable protection in peanut against peanut bud necrosis virus Rao, S.C. Bhatnagar-Mathur, P. Kumar, P. Lava Reddy, S. Sharma, K. peanut genetics Genetic engineering of peanut (Arachis hypogaea L.) using the gene encoding for the nucleocapsid protein (N gene) of peanut bud necrosis virus (PBNV; genus Tospovirus, family Bunyaviridae) was used to impart resistance to bud necrosis disease in peanut (PBND), a disease for which no durable resistance is available in the existing germplasm. Over 200 transgenic lines of peanut var. JL 24 were developed for which integration and expression of the transgenes was confirmed by PCR, Southern hybridization, RT-PCR and western blot analysis. The T1 and T2 generation transgenic plants were assayed through virus challenge in the greenhouse by using mechanical sap inoculation at 1:100 and 1:50 dilutions of PBNV, and they showed varying levels of disease incidence and intensity. Greenhouse and field evaluation with T2 generation plants indicated somewhat superior performance of the three transgenic events that showed considerable reduction in disease incidence. However, only one of these events showed over 75 % reduction in disease incidence when compared to the untransformed control, indicating partial and non-durable resistance to PBND using the viral N-gene. 2013-01 2016-07-11T08:41:26Z 2016-07-11T08:41:26Z Journal Article https://hdl.handle.net/10568/76031 en Limited Access Springer Rao, S. C., Bhatnagar-Mathur, P., Kumar, P. L., Reddy, A. S. & Sharma, K. K. (2013). Pathogen-derived resistance using a viral nucleocapsid gene confers only partial non-durable protection in peanut against peanut bud necrosis virus. Archives of Virology, 158(1), 133-143. |
| spellingShingle | peanut genetics Rao, S.C. Bhatnagar-Mathur, P. Kumar, P. Lava Reddy, S. Sharma, K. Pathogenderived resistance using a viral nucleocapsid gene confers only partial nondurable protection in peanut against peanut bud necrosis virus |
| title | Pathogenderived resistance using a viral nucleocapsid gene confers only partial nondurable protection in peanut against peanut bud necrosis virus |
| title_full | Pathogenderived resistance using a viral nucleocapsid gene confers only partial nondurable protection in peanut against peanut bud necrosis virus |
| title_fullStr | Pathogenderived resistance using a viral nucleocapsid gene confers only partial nondurable protection in peanut against peanut bud necrosis virus |
| title_full_unstemmed | Pathogenderived resistance using a viral nucleocapsid gene confers only partial nondurable protection in peanut against peanut bud necrosis virus |
| title_short | Pathogenderived resistance using a viral nucleocapsid gene confers only partial nondurable protection in peanut against peanut bud necrosis virus |
| title_sort | pathogenderived resistance using a viral nucleocapsid gene confers only partial nondurable protection in peanut against peanut bud necrosis virus |
| topic | peanut genetics |
| url | https://hdl.handle.net/10568/76031 |
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