Pathogenic variation of Mycosphaerella species infecting banana and plantain in Nigeria

Mycosphaerella species that cause the ‘Sigatoka disease complex’ account for significant yield losses in banana and plantain worldwide. Disease surveys were conducted in the humid forest (HF) and derived savanna (DS) agroecological zones from 2004 to 2006 to determine the distribution of the disease...

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Autores principales: Zandjanakou-Tachin, Martine, Ojiambo, P., Vroh Bi, Irie, Tenkouano, A., Gumedzoe, M.Y.D., Bandyopadhyay, Ranajit
Formato: Journal Article
Lenguaje:Inglés
Publicado: Wiley 2013
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Acceso en línea:https://hdl.handle.net/10568/76026
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author Zandjanakou-Tachin, Martine
Ojiambo, P.
Vroh Bi, Irie
Tenkouano, A.
Gumedzoe, M.Y.D.
Bandyopadhyay, Ranajit
author_browse Bandyopadhyay, Ranajit
Gumedzoe, M.Y.D.
Ojiambo, P.
Tenkouano, A.
Vroh Bi, Irie
Zandjanakou-Tachin, Martine
author_facet Zandjanakou-Tachin, Martine
Ojiambo, P.
Vroh Bi, Irie
Tenkouano, A.
Gumedzoe, M.Y.D.
Bandyopadhyay, Ranajit
author_sort Zandjanakou-Tachin, Martine
collection Repository of Agricultural Research Outputs (CGSpace)
description Mycosphaerella species that cause the ‘Sigatoka disease complex’ account for significant yield losses in banana and plantain worldwide. Disease surveys were conducted in the humid forest (HF) and derived savanna (DS) agroecological zones from 2004 to 2006 to determine the distribution of the disease and variation among Mycosphaerella species in Nigeria. Disease prevalence and severity were higher in the HF than in the DS zone, but significant (P < 0Æ001) differences between agroecological zones were only observed for disease severity. A total of 85 isolates of M. fijiensis and 11 isolates of M. eumusae were collected during the survey and used to characterize the pathogenic structure of Mycosphaerella spp. using a putative host differential cultivar set consisting of Calcutta-4 (resistant), Valery (intermediate) and Agbagba (highly susceptible). Area under disease progress curve (AUDPC) was higher on all cultivars when inoculated with M. eumusae than with M. fijiensis, but significant (P < 0Æ05) differences between the two species were only observed on Valery. Based on the rank-sum method, 8Æ3% of the isolates were classified as highly aggressive and 46Æ9% were classified as aggressive. About 11Æ5% of all the isolates were classified as least aggressive, and all of these were M. fijiensis. Themajority of M. eumusae isolates (seven out of 11; 64%) were classified as aggressive. A total of nine pathotype clusters were identified using cluster analysis of AUDPC. At least one M. fijiensis isolate was present in all the nine pathotype clusters, while isolates of M. eumusae were present in six of the nine clusters. Isolates in pathotype clusters III and V were the most aggressive, while those in cluster VIII were the least aggressive. Shannon’s index (H) revealed a more diverse Mycosphaerella collection in the DS zone (H = 1Æ81) than in the HF (H = 1Æ50) zone, with M. fijiensis being more diverse than M. eumusae. These results describe the current pathotype structure of Mycosphaerella in Nigeria and provide a useful resource that will facilitate screening of newly developed Musa genotypes for resistance against two important leaf spot diseases of banana and plantain.
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spelling CGSpace760262024-08-27T10:35:15Z Pathogenic variation of Mycosphaerella species infecting banana and plantain in Nigeria Zandjanakou-Tachin, Martine Ojiambo, P. Vroh Bi, Irie Tenkouano, A. Gumedzoe, M.Y.D. Bandyopadhyay, Ranajit musa mycosphaerella fijiensis pathogens pathotypes horticulture genetics Mycosphaerella species that cause the ‘Sigatoka disease complex’ account for significant yield losses in banana and plantain worldwide. Disease surveys were conducted in the humid forest (HF) and derived savanna (DS) agroecological zones from 2004 to 2006 to determine the distribution of the disease and variation among Mycosphaerella species in Nigeria. Disease prevalence and severity were higher in the HF than in the DS zone, but significant (P < 0Æ001) differences between agroecological zones were only observed for disease severity. A total of 85 isolates of M. fijiensis and 11 isolates of M. eumusae were collected during the survey and used to characterize the pathogenic structure of Mycosphaerella spp. using a putative host differential cultivar set consisting of Calcutta-4 (resistant), Valery (intermediate) and Agbagba (highly susceptible). Area under disease progress curve (AUDPC) was higher on all cultivars when inoculated with M. eumusae than with M. fijiensis, but significant (P < 0Æ05) differences between the two species were only observed on Valery. Based on the rank-sum method, 8Æ3% of the isolates were classified as highly aggressive and 46Æ9% were classified as aggressive. About 11Æ5% of all the isolates were classified as least aggressive, and all of these were M. fijiensis. Themajority of M. eumusae isolates (seven out of 11; 64%) were classified as aggressive. A total of nine pathotype clusters were identified using cluster analysis of AUDPC. At least one M. fijiensis isolate was present in all the nine pathotype clusters, while isolates of M. eumusae were present in six of the nine clusters. Isolates in pathotype clusters III and V were the most aggressive, while those in cluster VIII were the least aggressive. Shannon’s index (H) revealed a more diverse Mycosphaerella collection in the DS zone (H = 1Æ81) than in the HF (H = 1Æ50) zone, with M. fijiensis being more diverse than M. eumusae. These results describe the current pathotype structure of Mycosphaerella in Nigeria and provide a useful resource that will facilitate screening of newly developed Musa genotypes for resistance against two important leaf spot diseases of banana and plantain. 2013-04 2016-07-11T08:41:23Z 2016-07-11T08:41:23Z Journal Article https://hdl.handle.net/10568/76026 en Limited Access Wiley Zandjanakou?Tachin, M., Ojiambo, P. S., Vroh?Bi, I., Tenkouano, A., Gumedzoe, Y. M., & Bandyopadhyay, R. (2013). Pathogenic variation of Mycosphaerella species infecting banana and plantain in Nigeria. Plant Pathology, 62(2), 298-308.
spellingShingle musa
mycosphaerella fijiensis
pathogens
pathotypes
horticulture
genetics
Zandjanakou-Tachin, Martine
Ojiambo, P.
Vroh Bi, Irie
Tenkouano, A.
Gumedzoe, M.Y.D.
Bandyopadhyay, Ranajit
Pathogenic variation of Mycosphaerella species infecting banana and plantain in Nigeria
title Pathogenic variation of Mycosphaerella species infecting banana and plantain in Nigeria
title_full Pathogenic variation of Mycosphaerella species infecting banana and plantain in Nigeria
title_fullStr Pathogenic variation of Mycosphaerella species infecting banana and plantain in Nigeria
title_full_unstemmed Pathogenic variation of Mycosphaerella species infecting banana and plantain in Nigeria
title_short Pathogenic variation of Mycosphaerella species infecting banana and plantain in Nigeria
title_sort pathogenic variation of mycosphaerella species infecting banana and plantain in nigeria
topic musa
mycosphaerella fijiensis
pathogens
pathotypes
horticulture
genetics
url https://hdl.handle.net/10568/76026
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