First report of Xanthomonas euvesicatoria causing bacterial spot disease in pepper in Northwestern Nigeria
Bacterial spot (BS) has been reported as an important disease on pepper in Nigeria (4). Xanthomonas campestris pv. vesicatoria was identified as the causal agent using phenotypic and pathogenicity tests; however, X. campestris pv. vesicatoria is a synonym for two genetically distinct groups that hav...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Scientific Societies
2014
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/75951 |
| _version_ | 1855528132244668416 |
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| author | Jibrin, M.O. Timilsina, S. Potnis, N. Minsavage, G.V. Shenge, K.C. Akpa, A.D. Alegbejo, M. Beed, Fenton D. Vallad, G.E. Jones, J.B. |
| author_browse | Akpa, A.D. Alegbejo, M. Beed, Fenton D. Jibrin, M.O. Jones, J.B. Minsavage, G.V. Potnis, N. Shenge, K.C. Timilsina, S. Vallad, G.E. |
| author_facet | Jibrin, M.O. Timilsina, S. Potnis, N. Minsavage, G.V. Shenge, K.C. Akpa, A.D. Alegbejo, M. Beed, Fenton D. Vallad, G.E. Jones, J.B. |
| author_sort | Jibrin, M.O. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Bacterial spot (BS) has been reported as an important disease on pepper in Nigeria (4). Xanthomonas campestris pv. vesicatoria was identified as the causal agent using phenotypic and pathogenicity tests; however, X. campestris pv. vesicatoria is a synonym for two genetically distinct groups that have been elevated to the species X. euvesicatoria and X. vesicatoria (2). Furthermore, the latter two species and X. gardneri cause similar diseases on pepper (2). In order to determine the species associated with BS on pepper, leaves with irregular, dark brown lesions were collected from pepper plants in fields from northwestern Nigeria, and isolations were made on nutrient agar (NA). Yellow, mucoid colonies typical of Xanthomonas were isolated. Six strains isolated from pepper were determined to be non-amylolytic. For race determinations, bacterial suspensions of the pepper strains, derived from 24-h cultures grown on NA at 28°C, were adjusted to 108 CFU/ml and infiltrated into leaves of tomato and pepper differential genotypes (5). The six pepper strains elicited HRs on the tomato differential genotypes. The strains produced a susceptible reaction on all pepper differentials and were designated as pepper race 6 (5). Multilocus sequence analysis (MLSA) using six housekeeping genes (fusA, lacF, gyrB, gltA, gapA, and lepA) was used to further analyze the strains (1) (GenBank Accession Nos. KJ938585 to KJ938587, KJ938592 to KJ938594, KJ938599 to KJ938601, KJ938606 to KJ938608, KJ938633 to KJ938635, and KJ938640 to KJ938642). A partial sequence of hrpB2 was also sequenced since the four Xanthomonas species associated with BS can be differentiated based on sequence divergence (3) (KJ938622 to KJ938627). The housekeeping gene sequences were aligned along with other Xanthomonas sequences imported from the NCBI database using muscle tool from MEGA software, 5.2.2. Maximum likelihood phylogenetic trees constructed for the six housekeeping gene sequences individually and in concatenation revealed that the Nigerian pepper strains were identical to the X. euvesicatoria reference strain 85-10. Although BS is common in Nigeria, to our knowledge, this represents the first report for this pepper pathogen in Nigeria.References: (1) N. F. Almeida et al. Phytopathology 100:208, 2010. (3) J. B. Jones et al. System Appl. Microbiol. 27:755, 2004. (4) A. Obradovic et al. Eur. J. Plant Pathol. 88:736, 2004. (2) E. U. Opara and F. J. Odibo. J. Mol. Gen. 1:35, 2009. (5) R. E. Stall et al. Ann. Rev. Phytopathol. 47:265, 2009. |
| format | Journal Article |
| id | CGSpace75951 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace759512025-11-11T10:31:33Z First report of Xanthomonas euvesicatoria causing bacterial spot disease in pepper in Northwestern Nigeria Jibrin, M.O. Timilsina, S. Potnis, N. Minsavage, G.V. Shenge, K.C. Akpa, A.D. Alegbejo, M. Beed, Fenton D. Vallad, G.E. Jones, J.B. xanthomonas campestris vesicatoria pepper plant diseases Bacterial spot (BS) has been reported as an important disease on pepper in Nigeria (4). Xanthomonas campestris pv. vesicatoria was identified as the causal agent using phenotypic and pathogenicity tests; however, X. campestris pv. vesicatoria is a synonym for two genetically distinct groups that have been elevated to the species X. euvesicatoria and X. vesicatoria (2). Furthermore, the latter two species and X. gardneri cause similar diseases on pepper (2). In order to determine the species associated with BS on pepper, leaves with irregular, dark brown lesions were collected from pepper plants in fields from northwestern Nigeria, and isolations were made on nutrient agar (NA). Yellow, mucoid colonies typical of Xanthomonas were isolated. Six strains isolated from pepper were determined to be non-amylolytic. For race determinations, bacterial suspensions of the pepper strains, derived from 24-h cultures grown on NA at 28°C, were adjusted to 108 CFU/ml and infiltrated into leaves of tomato and pepper differential genotypes (5). The six pepper strains elicited HRs on the tomato differential genotypes. The strains produced a susceptible reaction on all pepper differentials and were designated as pepper race 6 (5). Multilocus sequence analysis (MLSA) using six housekeeping genes (fusA, lacF, gyrB, gltA, gapA, and lepA) was used to further analyze the strains (1) (GenBank Accession Nos. KJ938585 to KJ938587, KJ938592 to KJ938594, KJ938599 to KJ938601, KJ938606 to KJ938608, KJ938633 to KJ938635, and KJ938640 to KJ938642). A partial sequence of hrpB2 was also sequenced since the four Xanthomonas species associated with BS can be differentiated based on sequence divergence (3) (KJ938622 to KJ938627). The housekeeping gene sequences were aligned along with other Xanthomonas sequences imported from the NCBI database using muscle tool from MEGA software, 5.2.2. Maximum likelihood phylogenetic trees constructed for the six housekeeping gene sequences individually and in concatenation revealed that the Nigerian pepper strains were identical to the X. euvesicatoria reference strain 85-10. Although BS is common in Nigeria, to our knowledge, this represents the first report for this pepper pathogen in Nigeria.References: (1) N. F. Almeida et al. Phytopathology 100:208, 2010. (3) J. B. Jones et al. System Appl. Microbiol. 27:755, 2004. (4) A. Obradovic et al. Eur. J. Plant Pathol. 88:736, 2004. (2) E. U. Opara and F. J. Odibo. J. Mol. Gen. 1:35, 2009. (5) R. E. Stall et al. Ann. Rev. Phytopathol. 47:265, 2009. 2014-10 2016-07-04T08:15:41Z 2016-07-04T08:15:41Z Journal Article https://hdl.handle.net/10568/75951 en Open Access application/pdf Scientific Societies Jibrin, M.O., Timilsina, S., Potnis, N., Minsavage, G.V., Shenge, K.C., Akpa, A.D., Alegbejo, M., Beed, F., Vallad, G.E. & Jones, J.B. (2014). First report of Xanthomonas euvesicatoria causing bacterial spot disease in pepper in northwestern Nigeria. Plant Disease, 98(10), 1426-1426. |
| spellingShingle | xanthomonas campestris vesicatoria pepper plant diseases Jibrin, M.O. Timilsina, S. Potnis, N. Minsavage, G.V. Shenge, K.C. Akpa, A.D. Alegbejo, M. Beed, Fenton D. Vallad, G.E. Jones, J.B. First report of Xanthomonas euvesicatoria causing bacterial spot disease in pepper in Northwestern Nigeria |
| title | First report of Xanthomonas euvesicatoria causing bacterial spot disease in pepper in Northwestern Nigeria |
| title_full | First report of Xanthomonas euvesicatoria causing bacterial spot disease in pepper in Northwestern Nigeria |
| title_fullStr | First report of Xanthomonas euvesicatoria causing bacterial spot disease in pepper in Northwestern Nigeria |
| title_full_unstemmed | First report of Xanthomonas euvesicatoria causing bacterial spot disease in pepper in Northwestern Nigeria |
| title_short | First report of Xanthomonas euvesicatoria causing bacterial spot disease in pepper in Northwestern Nigeria |
| title_sort | first report of xanthomonas euvesicatoria causing bacterial spot disease in pepper in northwestern nigeria |
| topic | xanthomonas campestris vesicatoria pepper plant diseases |
| url | https://hdl.handle.net/10568/75951 |
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