Identification of F1 cassava (Manihot esculenta Crantz) progeny using microsatellite markers and capillary electrophoresis
Generation of genetic diversity is necessary in improving on the potential of cassava when faced with various biotic and abiotic challenges. Presently, cassava breeders are breeding for a number of traits, such as drought tolerance, early root bulking, yield, starch, beta-carotene, protein, dry matt...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Scientific Research Publishing, Inc.
2014
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/75939 |
| _version_ | 1855515226248577024 |
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| author | Kyaligonza, V. Kawuki, Robert S. Ferguson, Morag E. Kaweesi, T. Baguma, Yona K. Vuzi, P. |
| author_browse | Baguma, Yona K. Ferguson, Morag E. Kaweesi, T. Kawuki, Robert S. Kyaligonza, V. Vuzi, P. |
| author_facet | Kyaligonza, V. Kawuki, Robert S. Ferguson, Morag E. Kaweesi, T. Baguma, Yona K. Vuzi, P. |
| author_sort | Kyaligonza, V. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Generation of genetic diversity is necessary in improving on the potential of cassava when faced with various biotic and abiotic challenges. Presently, cassava breeders are breeding for a number of traits, such as drought tolerance, early root bulking, yield, starch, beta-carotene, protein, dry matter, pest and disease resistance, by relying on genetic diversity that exists in manihot esculenta germplasm. Controlled pollination is one of the main methods used to generate genetic diversity in cassava. However, the process of controlled pollination especially in an open field is prone to contamination by illegitimate pollen right from the time of pollination, seed collection, nursery bed establishment to planting of the trials. Therefore, authentication of the progeny obtained from cas-sava crosses is very important for genetic studies. Twelve informative microsatellite markers were used to verify the authenticity of 364 F1 progeny thought to come from four controlled parental crosses. The transmission of each allele at nine microsatellite loci was tracked from parents to progeny in each of the four Namikonga-derived F1 cassava families. Out of the 364 F1 progeny, 317 (87.1%) were true-to-type, 44 (12.1%) were a product of self-pollination and 3 (0.8%) were a product of open pollination. The consistency of the results obtained using microsatellite markers makes this technique a reliable tool for assessing the purity of progeny generated from cassava crosses. |
| format | Journal Article |
| id | CGSpace75939 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Scientific Research Publishing, Inc. |
| publisherStr | Scientific Research Publishing, Inc. |
| record_format | dspace |
| spelling | CGSpace759392025-11-11T10:03:17Z Identification of F1 cassava (Manihot esculenta Crantz) progeny using microsatellite markers and capillary electrophoresis Kyaligonza, V. Kawuki, Robert S. Ferguson, Morag E. Kaweesi, T. Baguma, Yona K. Vuzi, P. progeny genetic markers Generation of genetic diversity is necessary in improving on the potential of cassava when faced with various biotic and abiotic challenges. Presently, cassava breeders are breeding for a number of traits, such as drought tolerance, early root bulking, yield, starch, beta-carotene, protein, dry matter, pest and disease resistance, by relying on genetic diversity that exists in manihot esculenta germplasm. Controlled pollination is one of the main methods used to generate genetic diversity in cassava. However, the process of controlled pollination especially in an open field is prone to contamination by illegitimate pollen right from the time of pollination, seed collection, nursery bed establishment to planting of the trials. Therefore, authentication of the progeny obtained from cas-sava crosses is very important for genetic studies. Twelve informative microsatellite markers were used to verify the authenticity of 364 F1 progeny thought to come from four controlled parental crosses. The transmission of each allele at nine microsatellite loci was tracked from parents to progeny in each of the four Namikonga-derived F1 cassava families. Out of the 364 F1 progeny, 317 (87.1%) were true-to-type, 44 (12.1%) were a product of self-pollination and 3 (0.8%) were a product of open pollination. The consistency of the results obtained using microsatellite markers makes this technique a reliable tool for assessing the purity of progeny generated from cassava crosses. 2014 2016-07-04T08:15:35Z 2016-07-04T08:15:35Z Journal Article https://hdl.handle.net/10568/75939 en Open Access application/pdf Scientific Research Publishing, Inc. Kyaligonza, V., Kawuki, R.S., Ferguson, M., Kaweesi, T., Baguma, Y. & Vuzi, P.(2014). Identification of F1 Cassava (Manihot esculenta Crantz) progeny using microsatellite markers and capillary electrophoresis. American Journal of Plant Sciences, 5, 119-125. |
| spellingShingle | progeny genetic markers Kyaligonza, V. Kawuki, Robert S. Ferguson, Morag E. Kaweesi, T. Baguma, Yona K. Vuzi, P. Identification of F1 cassava (Manihot esculenta Crantz) progeny using microsatellite markers and capillary electrophoresis |
| title | Identification of F1 cassava (Manihot esculenta Crantz) progeny using microsatellite markers and capillary electrophoresis |
| title_full | Identification of F1 cassava (Manihot esculenta Crantz) progeny using microsatellite markers and capillary electrophoresis |
| title_fullStr | Identification of F1 cassava (Manihot esculenta Crantz) progeny using microsatellite markers and capillary electrophoresis |
| title_full_unstemmed | Identification of F1 cassava (Manihot esculenta Crantz) progeny using microsatellite markers and capillary electrophoresis |
| title_short | Identification of F1 cassava (Manihot esculenta Crantz) progeny using microsatellite markers and capillary electrophoresis |
| title_sort | identification of f1 cassava manihot esculenta crantz progeny using microsatellite markers and capillary electrophoresis |
| topic | progeny genetic markers |
| url | https://hdl.handle.net/10568/75939 |
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