Mortality of Maruca vitrata (Lepidoptera: Crambidae) larval stages induced by different doses of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana

Thedose-dependent mortality of second (3-4 days old),third (5-6 days old) and fourth (7-9 days old) larval instars of Marucavitrataas induced by the entomopathogenic fungal isolates, MetarhiziumanisopliaeMa 29 and BeauveriabassianaBb 115 was determined in a laboratory experiment. For eac...

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Main Authors: Mehinto, J.T., Atachi, P., Douro-Kpindou, O.K., Dannou, E.A., Tamò, Manuele
Format: Journal Article
Language:Inglés
Published: 2014
Subjects:
Online Access:https://hdl.handle.net/10568/75928
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author Mehinto, J.T.
Atachi, P.
Douro-Kpindou, O.K.
Dannou, E.A.
Tamò, Manuele
author_browse Atachi, P.
Dannou, E.A.
Douro-Kpindou, O.K.
Mehinto, J.T.
Tamò, Manuele
author_facet Mehinto, J.T.
Atachi, P.
Douro-Kpindou, O.K.
Dannou, E.A.
Tamò, Manuele
author_sort Mehinto, J.T.
collection Repository of Agricultural Research Outputs (CGSpace)
description Thedose-dependent mortality of second (3-4 days old),third (5-6 days old) and fourth (7-9 days old) larval instars of Marucavitrataas induced by the entomopathogenic fungal isolates, MetarhiziumanisopliaeMa 29 and BeauveriabassianaBb 115 was determined in a laboratory experiment. For each fungal isolate, five doses (0, 2x104, 2x105, 2x106and 2x107conidia/larva, respectively) were applied topically to one individual larva at the rate of 2 ?l ofan oil formulation. Observations were focused on mortality and sporulation of treated larvae. Cox regression was used to determine the lethal dose 50 (LD50). Results revealed high mortality of the third larval stage (L3) at 2 x 10?conidia for Ma 29 and Bb 115 with 49% and 96% mortality, respectively. No significant difference in mortality was observed between larval stages exposed to 2x107 conidia/ larva. The sporulation rate did not differ significantly between larval stages. Regardless of application doses, the Mean Survival Times (MSTs) of larvae were significantly different between doses for both fungal species. The lowest MSTs were recorded for the younger larvae (L2, L3) at higher doses (2x106 and 2x107 conidia/larva). Larval mortality increased with increasing dose, regardless of fungal species and larval stages, suggesting a dose-dependent response of M. vitrata larvae.
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spelling CGSpace759282025-10-16T09:52:51Z Mortality of Maruca vitrata (Lepidoptera: Crambidae) larval stages induced by different doses of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana Mehinto, J.T. Atachi, P. Douro-Kpindou, O.K. Dannou, E.A. Tamò, Manuele maruca vitrata metarhizium anisopliae beauveria bassiana Thedose-dependent mortality of second (3-4 days old),third (5-6 days old) and fourth (7-9 days old) larval instars of Marucavitrataas induced by the entomopathogenic fungal isolates, MetarhiziumanisopliaeMa 29 and BeauveriabassianaBb 115 was determined in a laboratory experiment. For each fungal isolate, five doses (0, 2x104, 2x105, 2x106and 2x107conidia/larva, respectively) were applied topically to one individual larva at the rate of 2 ?l ofan oil formulation. Observations were focused on mortality and sporulation of treated larvae. Cox regression was used to determine the lethal dose 50 (LD50). Results revealed high mortality of the third larval stage (L3) at 2 x 10?conidia for Ma 29 and Bb 115 with 49% and 96% mortality, respectively. No significant difference in mortality was observed between larval stages exposed to 2x107 conidia/ larva. The sporulation rate did not differ significantly between larval stages. Regardless of application doses, the Mean Survival Times (MSTs) of larvae were significantly different between doses for both fungal species. The lowest MSTs were recorded for the younger larvae (L2, L3) at higher doses (2x106 and 2x107 conidia/larva). Larval mortality increased with increasing dose, regardless of fungal species and larval stages, suggesting a dose-dependent response of M. vitrata larvae. 2014 2016-07-04T08:15:29Z 2016-07-04T08:15:29Z Journal Article https://hdl.handle.net/10568/75928 en Limited Access Mehinto, J.T., Atachi, P., Douro Kpindou, O., Dannon, E. & Tamò, M. (2014). Mortality of maruca vitrata (Lepidoptera: Crambidae) larval stages induced by different doses of the entomopathogenic fungi metarhizium anisopliae and Beauveria bassiana. International Journal, 2(4), 273-285.
spellingShingle maruca vitrata
metarhizium anisopliae
beauveria bassiana
Mehinto, J.T.
Atachi, P.
Douro-Kpindou, O.K.
Dannou, E.A.
Tamò, Manuele
Mortality of Maruca vitrata (Lepidoptera: Crambidae) larval stages induced by different doses of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana
title Mortality of Maruca vitrata (Lepidoptera: Crambidae) larval stages induced by different doses of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana
title_full Mortality of Maruca vitrata (Lepidoptera: Crambidae) larval stages induced by different doses of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana
title_fullStr Mortality of Maruca vitrata (Lepidoptera: Crambidae) larval stages induced by different doses of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana
title_full_unstemmed Mortality of Maruca vitrata (Lepidoptera: Crambidae) larval stages induced by different doses of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana
title_short Mortality of Maruca vitrata (Lepidoptera: Crambidae) larval stages induced by different doses of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana
title_sort mortality of maruca vitrata lepidoptera crambidae larval stages induced by different doses of the entomopathogenic fungi metarhizium anisopliae and beauveria bassiana
topic maruca vitrata
metarhizium anisopliae
beauveria bassiana
url https://hdl.handle.net/10568/75928
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