Evaluation of a lateral flow device for in-field detection of Banana Xanthomonas Wilt and its application in tracking the systemicity of Xanthomonas campestris pv. musacearum
Early detection of Banana Xanthomonas Wilt (BXW) in the field and immediate destruction of infected plants or plant tissue are key control methods to prevent the introduction and spread of BXW. This requires rapid, cost effective and an on-site diagnostic tool to detect the bacterium, Xanthomonas ca...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Academic Journals
2016
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/75877 |
| _version_ | 1855517401292996608 |
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| author | Karamura, G. Ochola, D. Smith, J. Kubiriba, Jerome Karamura, E.B. |
| author_browse | Karamura, E.B. Karamura, G. Kubiriba, Jerome Ochola, D. Smith, J. |
| author_facet | Karamura, G. Ochola, D. Smith, J. Kubiriba, Jerome Karamura, E.B. |
| author_sort | Karamura, G. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Early detection of Banana Xanthomonas Wilt (BXW) in the field and immediate destruction of infected plants or plant tissue are key control methods to prevent the introduction and spread of BXW. This requires rapid, cost effective and an on-site diagnostic tool to detect the bacterium, Xanthomonas campestris pv musacearum (Xcm). Polymerase chain reaction (PCR) detection technique for BXW is efficient but requires expensive equipment and knowledgeable expertise; this limits PCR application to the laboratory. This study therefore was carried out to evaluate the enzyme - linked immunosorbent assay (ELISA) tool configured as a lateral flow device (LFD) for detection of Xcm. Studies on the systemicity of Xcm in banana were carried out using the BXW - LFD in a field trial of 300 banana plants of Pisang Awak inoculated with the Xcm at Kiifu Forest, Mukono District, Uganda. Pseudo-stem samples from symptomatic and asymptomatic suckers were collected and tested with the LFD and the results compared with conventional PCR using the GspDm BXW primers. The LFD was able to detect Xcm3. |
| format | Journal Article |
| id | CGSpace75877 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Academic Journals |
| publisherStr | Academic Journals |
| record_format | dspace |
| spelling | CGSpace758772025-11-12T05:38:45Z Evaluation of a lateral flow device for in-field detection of Banana Xanthomonas Wilt and its application in tracking the systemicity of Xanthomonas campestris pv. musacearum Karamura, G. Ochola, D. Smith, J. Kubiriba, Jerome Karamura, E.B. xanthomonas campestris musa diagnostic techniques Early detection of Banana Xanthomonas Wilt (BXW) in the field and immediate destruction of infected plants or plant tissue are key control methods to prevent the introduction and spread of BXW. This requires rapid, cost effective and an on-site diagnostic tool to detect the bacterium, Xanthomonas campestris pv musacearum (Xcm). Polymerase chain reaction (PCR) detection technique for BXW is efficient but requires expensive equipment and knowledgeable expertise; this limits PCR application to the laboratory. This study therefore was carried out to evaluate the enzyme - linked immunosorbent assay (ELISA) tool configured as a lateral flow device (LFD) for detection of Xcm. Studies on the systemicity of Xcm in banana were carried out using the BXW - LFD in a field trial of 300 banana plants of Pisang Awak inoculated with the Xcm at Kiifu Forest, Mukono District, Uganda. Pseudo-stem samples from symptomatic and asymptomatic suckers were collected and tested with the LFD and the results compared with conventional PCR using the GspDm BXW primers. The LFD was able to detect Xcm3. 2016 2016-06-28T13:39:50Z 2016-06-28T13:39:50Z Journal Article https://hdl.handle.net/10568/75877 en Open Access application/pdf Academic Journals Karamura, G.; Ochola, D.; Smith, J.; Kubiriba, J.; Karamura, E. (2016) Evaluation of a lateral flow device for in-field detection of Banana Xanthomonas Wilt and its application in tracking the systemicity of Xanthomonas campestris pv. musacearum. African Journal of Agricultural Research 11(25) p. 2250-2256 ISSN: 1991-637X |
| spellingShingle | xanthomonas campestris musa diagnostic techniques Karamura, G. Ochola, D. Smith, J. Kubiriba, Jerome Karamura, E.B. Evaluation of a lateral flow device for in-field detection of Banana Xanthomonas Wilt and its application in tracking the systemicity of Xanthomonas campestris pv. musacearum |
| title | Evaluation of a lateral flow device for in-field detection of Banana Xanthomonas Wilt and its application in tracking the systemicity of Xanthomonas campestris pv. musacearum |
| title_full | Evaluation of a lateral flow device for in-field detection of Banana Xanthomonas Wilt and its application in tracking the systemicity of Xanthomonas campestris pv. musacearum |
| title_fullStr | Evaluation of a lateral flow device for in-field detection of Banana Xanthomonas Wilt and its application in tracking the systemicity of Xanthomonas campestris pv. musacearum |
| title_full_unstemmed | Evaluation of a lateral flow device for in-field detection of Banana Xanthomonas Wilt and its application in tracking the systemicity of Xanthomonas campestris pv. musacearum |
| title_short | Evaluation of a lateral flow device for in-field detection of Banana Xanthomonas Wilt and its application in tracking the systemicity of Xanthomonas campestris pv. musacearum |
| title_sort | evaluation of a lateral flow device for in field detection of banana xanthomonas wilt and its application in tracking the systemicity of xanthomonas campestris pv musacearum |
| topic | xanthomonas campestris musa diagnostic techniques |
| url | https://hdl.handle.net/10568/75877 |
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