Genome-wide association and prediction reveals genetic architecture of cassava mosaic disease resistance and prospects for rapid genetic improvement
Cassava (Manihot esculenta Crantz) is a crucial, under-researched crop feeding millions worldwide, especially in Africa. Cassava mosaic disease (CMD) has plagued production in Africa for over a century. Biparental mapping studies suggest primarily a single major gene mediates resistance. To investig...
| Autores principales: | , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2016
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/75810 |
| _version_ | 1855541057429700608 |
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| author | Wolfe, M.D. Rabbi, Ismail Y. Egesi, Chiedozie N. Hamblin, M. Kawuki, R.S. Kulakow, Peter A. Lozano, R. Carpio, D.P. del Ramu, P. Jannink, Jean-Luc |
| author_browse | Carpio, D.P. del Egesi, Chiedozie N. Hamblin, M. Jannink, Jean-Luc Kawuki, R.S. Kulakow, Peter A. Lozano, R. Rabbi, Ismail Y. Ramu, P. Wolfe, M.D. |
| author_facet | Wolfe, M.D. Rabbi, Ismail Y. Egesi, Chiedozie N. Hamblin, M. Kawuki, R.S. Kulakow, Peter A. Lozano, R. Carpio, D.P. del Ramu, P. Jannink, Jean-Luc |
| author_sort | Wolfe, M.D. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava (Manihot esculenta Crantz) is a crucial, under-researched crop feeding millions worldwide, especially in Africa. Cassava mosaic disease (CMD) has plagued production in Africa for over a century. Biparental mapping studies suggest primarily a single major gene mediates resistance. To investigate this genetic architecture, we conducted the first genome-wide association mapping study in cassava with up to 6128 genotyping-by-sequenced African breeding lines and 42,113 reference genome-mapped single-nucleotide polymorphism (SNP) markers. We found a single region on chromosome 8 that accounts for 30 to 66% of genetic resistance in the African cassava germplasm. Thirteen additional regions with small effects were also identified. Further dissection of the major quantitative trait locus (QTL) on chromosome 8 revealed the presence of two possibly epistatic loci and/or multiple resistance alleles, which may account for the difference between moderate and strong disease resistances in the germplasm. Search of potential candidate genes in the major QTL region identified two peroxidases and one thioredoxin. Finally, we found genomic prediction accuracy of 0.53 to 0.58 suggesting that genomic selection (GS) will be effective both for improving resistance in breeding populations and identifying highly resistant clones as varieties. |
| format | Journal Article |
| id | CGSpace75810 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace758102025-11-11T11:05:15Z Genome-wide association and prediction reveals genetic architecture of cassava mosaic disease resistance and prospects for rapid genetic improvement Wolfe, M.D. Rabbi, Ismail Y. Egesi, Chiedozie N. Hamblin, M. Kawuki, R.S. Kulakow, Peter A. Lozano, R. Carpio, D.P. del Ramu, P. Jannink, Jean-Luc genetic gain germplasm collections Cassava (Manihot esculenta Crantz) is a crucial, under-researched crop feeding millions worldwide, especially in Africa. Cassava mosaic disease (CMD) has plagued production in Africa for over a century. Biparental mapping studies suggest primarily a single major gene mediates resistance. To investigate this genetic architecture, we conducted the first genome-wide association mapping study in cassava with up to 6128 genotyping-by-sequenced African breeding lines and 42,113 reference genome-mapped single-nucleotide polymorphism (SNP) markers. We found a single region on chromosome 8 that accounts for 30 to 66% of genetic resistance in the African cassava germplasm. Thirteen additional regions with small effects were also identified. Further dissection of the major quantitative trait locus (QTL) on chromosome 8 revealed the presence of two possibly epistatic loci and/or multiple resistance alleles, which may account for the difference between moderate and strong disease resistances in the germplasm. Search of potential candidate genes in the major QTL region identified two peroxidases and one thioredoxin. Finally, we found genomic prediction accuracy of 0.53 to 0.58 suggesting that genomic selection (GS) will be effective both for improving resistance in breeding populations and identifying highly resistant clones as varieties. 2016-07 2016-06-23T11:16:26Z 2016-06-23T11:16:26Z Journal Article https://hdl.handle.net/10568/75810 en Open Access application/pdf Wiley Wolfe, M. D., Rabbi, I. Y., Egesi, C., Hamblin, M., Kawuki, R., Kulakow, P., ... & Jannink, J. L. (2016). Genome-Wide Association and Prediction Reveals Genetic Architecture of Cassava Mosaic Disease Resistance and Prospects for Rapid Genetic Improvement. The Plant Genome, 9(2), 1-13. |
| spellingShingle | genetic gain germplasm collections Wolfe, M.D. Rabbi, Ismail Y. Egesi, Chiedozie N. Hamblin, M. Kawuki, R.S. Kulakow, Peter A. Lozano, R. Carpio, D.P. del Ramu, P. Jannink, Jean-Luc Genome-wide association and prediction reveals genetic architecture of cassava mosaic disease resistance and prospects for rapid genetic improvement |
| title | Genome-wide association and prediction reveals genetic architecture of cassava mosaic disease resistance and prospects for rapid genetic improvement |
| title_full | Genome-wide association and prediction reveals genetic architecture of cassava mosaic disease resistance and prospects for rapid genetic improvement |
| title_fullStr | Genome-wide association and prediction reveals genetic architecture of cassava mosaic disease resistance and prospects for rapid genetic improvement |
| title_full_unstemmed | Genome-wide association and prediction reveals genetic architecture of cassava mosaic disease resistance and prospects for rapid genetic improvement |
| title_short | Genome-wide association and prediction reveals genetic architecture of cassava mosaic disease resistance and prospects for rapid genetic improvement |
| title_sort | genome wide association and prediction reveals genetic architecture of cassava mosaic disease resistance and prospects for rapid genetic improvement |
| topic | genetic gain germplasm collections |
| url | https://hdl.handle.net/10568/75810 |
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