Assessment of microbiological hazards along the milk value chain in Kilosa and Mvomero districts, Tanzania

The consumption of raw milk is a common practice among pastoral and agro-pastoral communities of Tanzania. This behaviour predisposes consumers to the risk of contracting milk-borne and zoonotic diseases. This study was carried out to assess milk quality based on identification of bacterial contamin...

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Main Author: Joseph, E.
Format: Tesis
Language:Inglés
Published: Sokoine University of Agriculture 2015
Subjects:
Online Access:https://hdl.handle.net/10568/74528
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author Joseph, E.
author_browse Joseph, E.
author_facet Joseph, E.
author_sort Joseph, E.
collection Repository of Agricultural Research Outputs (CGSpace)
description The consumption of raw milk is a common practice among pastoral and agro-pastoral communities of Tanzania. This behaviour predisposes consumers to the risk of contracting milk-borne and zoonotic diseases. This study was carried out to assess milk quality based on identification of bacterial contaminants indicated by total viable count (TVC), total coliform count (TCC) and contamination with Brucella and E. coli O157: H7 microorganisms. The study was carried out along the milk value chain (MVC) in Kilosa and Mvomero Districts of Morogoro Region in Tanzania. A total of 109 milk samples were collected along the MVC from farmers (54), milk vendors (31), milk collection centres (6) and milk selling points (18). Collected milk samples were subjected to TVC, TCC and polymerase chain reaction (PCR) to identify the presence of microorganisms in the milk. Laboratory findings indicate that milk from Kilosa district had significantly (p=0.015) higher TVC than milk from Mvomero district. The TVC varied significantly (p=0.00) along the MVC in the two districts. Using PCR, the overall prevalence of Brucella was 17.1% (n=82 out of 109), with the prevalence of 25.8% and 11.8% recorded in Kilosa and Mvomero districts, respectively. The E. coli O157:H7 was neither isolated nor detected in all 109 milk samples processed. Such findings suggest that milk marketed along the MVC is contaminated with Brucella organisms, thus posing public health risks to consumers. It is recommended that concerted efforts should be made to safeguard health of consumers through adopting various interventions that would reduce risks at each node along the MVC in the study area.
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spelling CGSpace745282025-11-05T04:04:53Z Assessment of microbiological hazards along the milk value chain in Kilosa and Mvomero districts, Tanzania Joseph, E. food safety The consumption of raw milk is a common practice among pastoral and agro-pastoral communities of Tanzania. This behaviour predisposes consumers to the risk of contracting milk-borne and zoonotic diseases. This study was carried out to assess milk quality based on identification of bacterial contaminants indicated by total viable count (TVC), total coliform count (TCC) and contamination with Brucella and E. coli O157: H7 microorganisms. The study was carried out along the milk value chain (MVC) in Kilosa and Mvomero Districts of Morogoro Region in Tanzania. A total of 109 milk samples were collected along the MVC from farmers (54), milk vendors (31), milk collection centres (6) and milk selling points (18). Collected milk samples were subjected to TVC, TCC and polymerase chain reaction (PCR) to identify the presence of microorganisms in the milk. Laboratory findings indicate that milk from Kilosa district had significantly (p=0.015) higher TVC than milk from Mvomero district. The TVC varied significantly (p=0.00) along the MVC in the two districts. Using PCR, the overall prevalence of Brucella was 17.1% (n=82 out of 109), with the prevalence of 25.8% and 11.8% recorded in Kilosa and Mvomero districts, respectively. The E. coli O157:H7 was neither isolated nor detected in all 109 milk samples processed. Such findings suggest that milk marketed along the MVC is contaminated with Brucella organisms, thus posing public health risks to consumers. It is recommended that concerted efforts should be made to safeguard health of consumers through adopting various interventions that would reduce risks at each node along the MVC in the study area. 2015 2016-05-29T08:19:32Z 2016-05-29T08:19:32Z Thesis https://hdl.handle.net/10568/74528 en Open Access application/pdf Sokoine University of Agriculture Joseph, E. 2015. Assessment of microbiological hazards along the milk value chain in Kilosa and Mvomero districts, Tanzania. MSc thesis. Morogoro, Tanzania: Sokoine University of Agriculture.
spellingShingle food safety
Joseph, E.
Assessment of microbiological hazards along the milk value chain in Kilosa and Mvomero districts, Tanzania
title Assessment of microbiological hazards along the milk value chain in Kilosa and Mvomero districts, Tanzania
title_full Assessment of microbiological hazards along the milk value chain in Kilosa and Mvomero districts, Tanzania
title_fullStr Assessment of microbiological hazards along the milk value chain in Kilosa and Mvomero districts, Tanzania
title_full_unstemmed Assessment of microbiological hazards along the milk value chain in Kilosa and Mvomero districts, Tanzania
title_short Assessment of microbiological hazards along the milk value chain in Kilosa and Mvomero districts, Tanzania
title_sort assessment of microbiological hazards along the milk value chain in kilosa and mvomero districts tanzania
topic food safety
url https://hdl.handle.net/10568/74528
work_keys_str_mv AT josephe assessmentofmicrobiologicalhazardsalongthemilkvaluechaininkilosaandmvomerodistrictstanzania