Transcriptomic profiling in Musa: a look into processes affected by mild osmotic stress in the root tip
Drought stress is one of the major abiotic factors limiting banana (Musa) production. Even mild-drought conditions are responsible for considerable yield losses. We performed large-scale transcriptome sequencing using Illumina technology on root tissue of three triploid genotypes representing well k...
| Autores principales: | , , , , , , , , , |
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| Formato: | Conference Paper |
| Lenguaje: | Inglés |
| Publicado: |
2016
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/74482 |
| _version_ | 1855526037709914112 |
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| author | Zorrilla-Fontanesi, Yasmín Rouard, M. Cenci, A. Kissel, E. Do, H. Dubois, E. Nidelet, S. Roux, N. Swennen, Rony L. Carpentier, Sebastien C. |
| author_browse | Carpentier, Sebastien C. Cenci, A. Do, H. Dubois, E. Kissel, E. Nidelet, S. Rouard, M. Roux, N. Swennen, Rony L. Zorrilla-Fontanesi, Yasmín |
| author_facet | Zorrilla-Fontanesi, Yasmín Rouard, M. Cenci, A. Kissel, E. Do, H. Dubois, E. Nidelet, S. Roux, N. Swennen, Rony L. Carpentier, Sebastien C. |
| author_sort | Zorrilla-Fontanesi, Yasmín |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Drought stress is one of the major abiotic factors limiting banana (Musa) production. Even mild-drought conditions are responsible for considerable yield losses. We performed large-scale transcriptome sequencing using Illumina technology on root tissue of three triploid genotypes representing well known cultivars and focused on the identification of genes with an altered expression pattern under mild osmotic stress (3 days after 5% PEG treatment). In total, 18 cDNA libraries were sequenced producing around 568 million high quality reads, of which 70-84% were mapped to the diploid reference genome (D’Hont et al., 2012). Through uni-/multivariate statistics, 92 genes were commonly identified as differentially expressed in the three genotypes. Using our in house workflow to analyze GO enriched and underlying biochemical pathways, we present a panorama of the general processes affected by mild osmotic stress in the root tip, although we observe a bias towards glycolysis and fermentation. We hypothesize that in this fast growing and oxygen demanding tissue, mild osmotic stress leads to a lower energy level, which induces a metabolic shift towards (i) a higher oxidative respiration, (ii) alternative respiration and (iii) fermentation. To validate the mRNA-seq results, a subset of twenty up-regulated genes were further analyzed at three different time points (6 hours, 3 days and 7 days) in an independent PEG experiment. Overall, the identification and annotation of this set of genes constitutes a step ahead to understand the complex network of root responses to osmotic (drought) stress. |
| format | Conference Paper |
| id | CGSpace74482 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| record_format | dspace |
| spelling | CGSpace744822023-09-25T09:16:55Z Transcriptomic profiling in Musa: a look into processes affected by mild osmotic stress in the root tip Zorrilla-Fontanesi, Yasmín Rouard, M. Cenci, A. Kissel, E. Do, H. Dubois, E. Nidelet, S. Roux, N. Swennen, Rony L. Carpentier, Sebastien C. rna sequence osmotic stress musa roots Drought stress is one of the major abiotic factors limiting banana (Musa) production. Even mild-drought conditions are responsible for considerable yield losses. We performed large-scale transcriptome sequencing using Illumina technology on root tissue of three triploid genotypes representing well known cultivars and focused on the identification of genes with an altered expression pattern under mild osmotic stress (3 days after 5% PEG treatment). In total, 18 cDNA libraries were sequenced producing around 568 million high quality reads, of which 70-84% were mapped to the diploid reference genome (D’Hont et al., 2012). Through uni-/multivariate statistics, 92 genes were commonly identified as differentially expressed in the three genotypes. Using our in house workflow to analyze GO enriched and underlying biochemical pathways, we present a panorama of the general processes affected by mild osmotic stress in the root tip, although we observe a bias towards glycolysis and fermentation. We hypothesize that in this fast growing and oxygen demanding tissue, mild osmotic stress leads to a lower energy level, which induces a metabolic shift towards (i) a higher oxidative respiration, (ii) alternative respiration and (iii) fermentation. To validate the mRNA-seq results, a subset of twenty up-regulated genes were further analyzed at three different time points (6 hours, 3 days and 7 days) in an independent PEG experiment. Overall, the identification and annotation of this set of genes constitutes a step ahead to understand the complex network of root responses to osmotic (drought) stress. 2016 2016-05-25T13:29:59Z 2016-05-25T13:29:59Z Conference Paper https://hdl.handle.net/10568/74482 en Open Access Zorrilla-Fontanesi, Y.; Rouard, M.; Cenci, A.; Kissel, E.; Do, H.; Dubois, E.; Nidelet, S.; Roux, N.; Swennen, R.; Carpentier, S.C. (2016) Transcriptomic profiling in Musa: a look into processes affected by mild osmotic stress in the root tip. [Abstract] XXIV Plant and Animal Genome Conference. San Diego, CA (USA) 9-13 Jan 2016. |
| spellingShingle | rna sequence osmotic stress musa roots Zorrilla-Fontanesi, Yasmín Rouard, M. Cenci, A. Kissel, E. Do, H. Dubois, E. Nidelet, S. Roux, N. Swennen, Rony L. Carpentier, Sebastien C. Transcriptomic profiling in Musa: a look into processes affected by mild osmotic stress in the root tip |
| title | Transcriptomic profiling in Musa: a look into processes affected by mild osmotic stress in the root tip |
| title_full | Transcriptomic profiling in Musa: a look into processes affected by mild osmotic stress in the root tip |
| title_fullStr | Transcriptomic profiling in Musa: a look into processes affected by mild osmotic stress in the root tip |
| title_full_unstemmed | Transcriptomic profiling in Musa: a look into processes affected by mild osmotic stress in the root tip |
| title_short | Transcriptomic profiling in Musa: a look into processes affected by mild osmotic stress in the root tip |
| title_sort | transcriptomic profiling in musa a look into processes affected by mild osmotic stress in the root tip |
| topic | rna sequence osmotic stress musa roots |
| url | https://hdl.handle.net/10568/74482 |
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