Highre-solution linkage map and chromosome-scale genome assembly for cassava (Manihot esculenta Crantz) from 10 populations

Cassava (Manihot esculenta Crantz) is a major staple crop in Africa, Asia, and South America, and its starchy roots provide nourishment for 800 million people worldwide. Although native to South America, cassava was brought to Africa 400–500 years ago and is now widely cultivated across sub-Sahara...

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Main Author: International Cassava Genetic Map Consortium
Format: Journal Article
Language:Inglés
Published: Oxford University Press 2015
Subjects:
Online Access:https://hdl.handle.net/10568/74475
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author International Cassava Genetic Map Consortium
author_browse International Cassava Genetic Map Consortium
author_facet International Cassava Genetic Map Consortium
author_sort International Cassava Genetic Map Consortium
collection Repository of Agricultural Research Outputs (CGSpace)
description Cassava (Manihot esculenta Crantz) is a major staple crop in Africa, Asia, and South America, and its starchy roots provide nourishment for 800 million people worldwide. Although native to South America, cassava was brought to Africa 400–500 years ago and is now widely cultivated across sub-Saharan Africa, but it is subject to biotic and abiotic stresses. To assist in the rapid identification of markers for pathogen resistance and crop traits, and to accelerate breeding programs, we generated a framework map for M. esculenta Crantz from reduced representation sequencing [genotyping-by-sequencing (GBS)]. The composite 2412-cM map integrates 10 biparental maps (comprising 3480 meioses) and organizes 22,403 genetic markers on 18 chromosomes, in agreement with the observed karyotype. We used the map to anchor 71.9% of the draft genome assembly and 90.7% of the predicted protein-coding genes. The chromosome-anchored genome sequence will be useful for breeding improvement by assisting in the rapid identification of markers linked to important traits, and in providing a framework for genomic selectionenhanced breeding of this important crop.
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spelling CGSpace744752023-12-08T19:36:04Z Highre-solution linkage map and chromosome-scale genome assembly for cassava (Manihot esculenta Crantz) from 10 populations International Cassava Genetic Map Consortium genetic map cassava Cassava (Manihot esculenta Crantz) is a major staple crop in Africa, Asia, and South America, and its starchy roots provide nourishment for 800 million people worldwide. Although native to South America, cassava was brought to Africa 400–500 years ago and is now widely cultivated across sub-Saharan Africa, but it is subject to biotic and abiotic stresses. To assist in the rapid identification of markers for pathogen resistance and crop traits, and to accelerate breeding programs, we generated a framework map for M. esculenta Crantz from reduced representation sequencing [genotyping-by-sequencing (GBS)]. The composite 2412-cM map integrates 10 biparental maps (comprising 3480 meioses) and organizes 22,403 genetic markers on 18 chromosomes, in agreement with the observed karyotype. We used the map to anchor 71.9% of the draft genome assembly and 90.7% of the predicted protein-coding genes. The chromosome-anchored genome sequence will be useful for breeding improvement by assisting in the rapid identification of markers linked to important traits, and in providing a framework for genomic selectionenhanced breeding of this important crop. 2015-01-01 2016-05-25T12:00:15Z 2016-05-25T12:00:15Z Journal Article https://hdl.handle.net/10568/74475 en Open Access application/octet-stream Oxford University Press International Cassava Genetic Map Consortium. (2015). High-resolution linkage map and chromosome-scale genome assembly for cassava (Manihot esculenta Crantz) from 10 Populations. G3: Genes| Genomes| Genetics, 5(1), 133-144.
spellingShingle genetic map
cassava
International Cassava Genetic Map Consortium
Highre-solution linkage map and chromosome-scale genome assembly for cassava (Manihot esculenta Crantz) from 10 populations
title Highre-solution linkage map and chromosome-scale genome assembly for cassava (Manihot esculenta Crantz) from 10 populations
title_full Highre-solution linkage map and chromosome-scale genome assembly for cassava (Manihot esculenta Crantz) from 10 populations
title_fullStr Highre-solution linkage map and chromosome-scale genome assembly for cassava (Manihot esculenta Crantz) from 10 populations
title_full_unstemmed Highre-solution linkage map and chromosome-scale genome assembly for cassava (Manihot esculenta Crantz) from 10 populations
title_short Highre-solution linkage map and chromosome-scale genome assembly for cassava (Manihot esculenta Crantz) from 10 populations
title_sort highre solution linkage map and chromosome scale genome assembly for cassava manihot esculenta crantz from 10 populations
topic genetic map
cassava
url https://hdl.handle.net/10568/74475
work_keys_str_mv AT internationalcassavageneticmapconsortium highresolutionlinkagemapandchromosomescalegenomeassemblyforcassavamanihotesculentacrantzfrom10populations