Analysis of genetic diversity of African yam bean using SSR markers derived from cowpea
African yam bean, AYB (Sphenostylis stenocarpa Hochst. ex. A. Rich Harms), is a tuberous legume of tropical Africa. AYB has the potential to significantly boost food security due to its considerable nutritional qualities. However, the crop is underutilized. To efficiently utilize AYB genetic resourc...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Cambridge University Press
2016
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/74458 |
| _version_ | 1855524164202397696 |
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| author | Shitta, N.S. Abberton, Michael T. Adesoye, A.I. Adewale, D.B. Oyatomi, Olaniyi |
| author_browse | Abberton, Michael T. Adesoye, A.I. Adewale, D.B. Oyatomi, Olaniyi Shitta, N.S. |
| author_facet | Shitta, N.S. Abberton, Michael T. Adesoye, A.I. Adewale, D.B. Oyatomi, Olaniyi |
| author_sort | Shitta, N.S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | African yam bean, AYB (Sphenostylis stenocarpa Hochst. ex. A. Rich Harms), is a tuberous
legume of tropical Africa. AYB has the potential to significantly boost food security due to
its considerable nutritional qualities. However, the crop is underutilized. To efficiently utilize
AYB genetic resources for its improvement, it is necessary to understand the crop’s diversity.
This study investigated the amplification ability of 36 cowpea simple sequence repeat (SSR)
primers across AYB genomic DNA, extracted from 67 accessions. Thirteen (36%) of the
cowpea SSRs showed transferability in AYB. Eight of these SSRs amplified above 60% of
AYB accessions and generated 55 polymorphic fragments with an average of 6.9 per primer.
Polymorphic information content ranged from 0.6691 to 0.8857 with an average of 0.7791.
This study also assessed the genetic diversity within 67 AYB accessions using eight cowpea
(Vigna unguiculata L. Walp)-derived SSR primers. The result revealed a high level of genetic
diversity with simple matching coefficient ranging from 0.458 to 1.000. A dendrogram depicting
three main clusters was generated based on unweighted pair group method with arithmetic
average. Cluster 1 was the most diverse with a dissimilarity range of 0.517–1.000. The level of
genetic diversity revealed in this study indicates that the studied AYB germplasm can be
exploited for genetic improvement. Additionally, the transferable markers will aid AYB
genome research and also make possible the comparative mapping between AYB and cowpea.African yam bean, AYB (Sphenostylis stenocarpa Hochst. ex. A. Rich Harms), is a tuberous
legume of tropical Africa. AYB has the potential to significantly boost food security due to
its considerable nutritional qualities. However, the crop is underutilized. To efficiently utilize
AYB genetic resources for its improvement, it is necessary to understand the crop’s diversity.
This study investigated the amplification ability of 36 cowpea simple sequence repeat (SSR)
primers across AYB genomic DNA, extracted from 67 accessions. Thirteen (36%) of the
cowpea SSRs showed transferability in AYB. Eight of these SSRs amplified above 60% of
AYB accessions and generated 55 polymorphic fragments with an average of 6.9 per primer.
Polymorphic information content ranged from 0.6691 to 0.8857 with an average of 0.7791.
This study also assessed the genetic diversity within 67 AYB accessions using eight cowpea
(Vigna unguiculata L. Walp)-derived SSR primers. The result revealed a high level of genetic
diversity with simple matching coefficient ranging from 0.458 to 1.000. A dendrogram depicting
three main clusters was generated based on unweighted pair group method with arithmetic
average. Cluster 1 was the most diverse with a dissimilarity range of 0.517–1.000. The level of
genetic diversity revealed in this study indicates that the studied AYB germplasm can be
exploited for genetic improvement. Additionally, the transferable markers will aid AYB
genome research and also make possible the comparative mapping between AYB and cowpea.African yam bean, AYB (Sphenostylis stenocarpa Hochst. ex. A. Rich Harms), is a tuberous
legume of tropical Africa. AYB has the potential to significantly boost food security due to
its considerable nutritional qualities. However, the crop is underutilized. To efficiently utilize
AYB genetic resources for its improvement, it is necessary to understand the crop’s diversity.
This study investigated the amplification ability of 36 cowpea simple sequence repeat (SSR)
primers across AYB genomic DNA, extracted from 67 accessions. Thirteen (36%) of the
cowpea SSRs showed transferability in AYB. Eight of these SSRs amplified above 60% of
AYB accessions and generated 55 polymorphic fragments with an average of 6.9 per primer.
Polymorphic information content ranged from 0.6691 to 0.8857 with an average of 0.7791.
This study also assessed the genetic diversity within 67 AYB accessions using eight cowpea
(Vigna unguiculata L. Walp)-derived SSR primers. The result revealed a high level of genetic
diversity with simple matching coefficient ranging from 0.458 to 1.000. A dendrogram depicting
three main clusters was generated based on unweighted pair group method with arithmetic
average. Cluster 1 was the most diverse with a dissimilarity range of 0.517–1.000. The level of
genetic diversity revealed in this study indicates that the studied AYB germplasm can be
exploited for genetic improvement. Additionally, the transferable markers will aid AYB
genome research and also make possible the comparative mapping between AYB and cowpea.African yam bean, AYB (Sphenostylis stenocarpa Hochst. ex. A. Rich Harms), is a tuberous
legume of tropical Africa. AYB has the potential to significantly boost food security due to
its considerable nutritional qualities. However, the crop is underutilized. To efficiently utilize
AYB genetic resources for its improvement, it is necessary to understand the crop’s diversity.
This study investigated the amplification ability of 36 cowpea simple sequence repeat (SSR)
primers across AYB genomic DNA, extracted from 67 accessions. Thirteen (36%) of the
cowpea SSRs showed transferability in AYB. Eight of these SSRs amplified above 60% of
AYB accessions and generated 55 polymorphic fragments with an average of 6.9 per primer.
Polymorphic information content ranged from 0.6691 to 0.8857 with an average of 0.7791.
This study also assessed the genetic diversity within 67 AYB accessions using eight cowpea
(Vigna unguiculata L. Walp)-derived SSR primers. The result revealed a high level of genetic
diversity with simple matching coefficient ranging from 0.458 to 1.000. A dendrogram depicting
three main clusters was generated based on unweighted pair group method with arithmetic
average. Cluster 1 was the most diverse with a dissimilarity range of 0.517–1.000. The level of
genetic diversity revealed in this study indicates that the studied AYB germplasm can be
exploited for genetic improvement. Additionally, the transferable markers will aid AYB
genome research and also make possible the comparative mapping between AYB and cowpea. |
| format | Journal Article |
| id | CGSpace74458 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace744582024-11-15T08:52:28Z Analysis of genetic diversity of African yam bean using SSR markers derived from cowpea Shitta, N.S. Abberton, Michael T. Adesoye, A.I. Adewale, D.B. Oyatomi, Olaniyi african yam bean cowpeas genetic variation African yam bean, AYB (Sphenostylis stenocarpa Hochst. ex. A. Rich Harms), is a tuberous legume of tropical Africa. AYB has the potential to significantly boost food security due to its considerable nutritional qualities. However, the crop is underutilized. To efficiently utilize AYB genetic resources for its improvement, it is necessary to understand the crop’s diversity. This study investigated the amplification ability of 36 cowpea simple sequence repeat (SSR) primers across AYB genomic DNA, extracted from 67 accessions. Thirteen (36%) of the cowpea SSRs showed transferability in AYB. Eight of these SSRs amplified above 60% of AYB accessions and generated 55 polymorphic fragments with an average of 6.9 per primer. Polymorphic information content ranged from 0.6691 to 0.8857 with an average of 0.7791. This study also assessed the genetic diversity within 67 AYB accessions using eight cowpea (Vigna unguiculata L. Walp)-derived SSR primers. The result revealed a high level of genetic diversity with simple matching coefficient ranging from 0.458 to 1.000. A dendrogram depicting three main clusters was generated based on unweighted pair group method with arithmetic average. Cluster 1 was the most diverse with a dissimilarity range of 0.517–1.000. The level of genetic diversity revealed in this study indicates that the studied AYB germplasm can be exploited for genetic improvement. Additionally, the transferable markers will aid AYB genome research and also make possible the comparative mapping between AYB and cowpea.African yam bean, AYB (Sphenostylis stenocarpa Hochst. ex. A. Rich Harms), is a tuberous legume of tropical Africa. AYB has the potential to significantly boost food security due to its considerable nutritional qualities. However, the crop is underutilized. To efficiently utilize AYB genetic resources for its improvement, it is necessary to understand the crop’s diversity. This study investigated the amplification ability of 36 cowpea simple sequence repeat (SSR) primers across AYB genomic DNA, extracted from 67 accessions. Thirteen (36%) of the cowpea SSRs showed transferability in AYB. Eight of these SSRs amplified above 60% of AYB accessions and generated 55 polymorphic fragments with an average of 6.9 per primer. Polymorphic information content ranged from 0.6691 to 0.8857 with an average of 0.7791. This study also assessed the genetic diversity within 67 AYB accessions using eight cowpea (Vigna unguiculata L. Walp)-derived SSR primers. The result revealed a high level of genetic diversity with simple matching coefficient ranging from 0.458 to 1.000. A dendrogram depicting three main clusters was generated based on unweighted pair group method with arithmetic average. Cluster 1 was the most diverse with a dissimilarity range of 0.517–1.000. The level of genetic diversity revealed in this study indicates that the studied AYB germplasm can be exploited for genetic improvement. Additionally, the transferable markers will aid AYB genome research and also make possible the comparative mapping between AYB and cowpea.African yam bean, AYB (Sphenostylis stenocarpa Hochst. ex. A. Rich Harms), is a tuberous legume of tropical Africa. AYB has the potential to significantly boost food security due to its considerable nutritional qualities. However, the crop is underutilized. To efficiently utilize AYB genetic resources for its improvement, it is necessary to understand the crop’s diversity. This study investigated the amplification ability of 36 cowpea simple sequence repeat (SSR) primers across AYB genomic DNA, extracted from 67 accessions. Thirteen (36%) of the cowpea SSRs showed transferability in AYB. Eight of these SSRs amplified above 60% of AYB accessions and generated 55 polymorphic fragments with an average of 6.9 per primer. Polymorphic information content ranged from 0.6691 to 0.8857 with an average of 0.7791. This study also assessed the genetic diversity within 67 AYB accessions using eight cowpea (Vigna unguiculata L. Walp)-derived SSR primers. The result revealed a high level of genetic diversity with simple matching coefficient ranging from 0.458 to 1.000. A dendrogram depicting three main clusters was generated based on unweighted pair group method with arithmetic average. Cluster 1 was the most diverse with a dissimilarity range of 0.517–1.000. The level of genetic diversity revealed in this study indicates that the studied AYB germplasm can be exploited for genetic improvement. Additionally, the transferable markers will aid AYB genome research and also make possible the comparative mapping between AYB and cowpea.African yam bean, AYB (Sphenostylis stenocarpa Hochst. ex. A. Rich Harms), is a tuberous legume of tropical Africa. AYB has the potential to significantly boost food security due to its considerable nutritional qualities. However, the crop is underutilized. To efficiently utilize AYB genetic resources for its improvement, it is necessary to understand the crop’s diversity. This study investigated the amplification ability of 36 cowpea simple sequence repeat (SSR) primers across AYB genomic DNA, extracted from 67 accessions. Thirteen (36%) of the cowpea SSRs showed transferability in AYB. Eight of these SSRs amplified above 60% of AYB accessions and generated 55 polymorphic fragments with an average of 6.9 per primer. Polymorphic information content ranged from 0.6691 to 0.8857 with an average of 0.7791. This study also assessed the genetic diversity within 67 AYB accessions using eight cowpea (Vigna unguiculata L. Walp)-derived SSR primers. The result revealed a high level of genetic diversity with simple matching coefficient ranging from 0.458 to 1.000. A dendrogram depicting three main clusters was generated based on unweighted pair group method with arithmetic average. Cluster 1 was the most diverse with a dissimilarity range of 0.517–1.000. The level of genetic diversity revealed in this study indicates that the studied AYB germplasm can be exploited for genetic improvement. Additionally, the transferable markers will aid AYB genome research and also make possible the comparative mapping between AYB and cowpea. 2016-03 2016-05-25T11:59:48Z 2016-05-25T11:59:48Z Journal Article https://hdl.handle.net/10568/74458 en Limited Access Cambridge University Press Shitta, N.S., Abberton, M.T., Adesoye, A.I., Adewale, D.B., & Oyatomi, O. (2015). Analysis of genetic diversity of African yam bean using SSR markers derived from cowpea. Plant Genetic Resources, 1-7. |
| spellingShingle | african yam bean cowpeas genetic variation Shitta, N.S. Abberton, Michael T. Adesoye, A.I. Adewale, D.B. Oyatomi, Olaniyi Analysis of genetic diversity of African yam bean using SSR markers derived from cowpea |
| title | Analysis of genetic diversity of African yam bean using SSR markers derived from cowpea |
| title_full | Analysis of genetic diversity of African yam bean using SSR markers derived from cowpea |
| title_fullStr | Analysis of genetic diversity of African yam bean using SSR markers derived from cowpea |
| title_full_unstemmed | Analysis of genetic diversity of African yam bean using SSR markers derived from cowpea |
| title_short | Analysis of genetic diversity of African yam bean using SSR markers derived from cowpea |
| title_sort | analysis of genetic diversity of african yam bean using ssr markers derived from cowpea |
| topic | african yam bean cowpeas genetic variation |
| url | https://hdl.handle.net/10568/74458 |
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