Fine mapping of Msv1, a major QTL for resistance to Maize Streak Virus leads to development of production markers for breeding pipelines
Abstract Maize streak virus (MSV) disease is a devastating disease in the Sub-Saharan Africa (SSA), which causes significant yield loss in maize. Resistance to MSV has previously been mapped to a major QTL (Msv1) on chromosome 1 that is germplasm and environment independent and to several minor loci...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2015
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/74431 |
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| author | Nair, S.K. Babu, R. Magorokosho, Cosmos Mahuku, George S. Semagn, K. Beyene, Y. Das, B. Makumbi, Dan Kumar, P. Lava Olsen, M. Boddupalli, P.M. |
| author_browse | Babu, R. Beyene, Y. Boddupalli, P.M. Das, B. Kumar, P. Lava Magorokosho, Cosmos Mahuku, George S. Makumbi, Dan Nair, S.K. Olsen, M. Semagn, K. |
| author_facet | Nair, S.K. Babu, R. Magorokosho, Cosmos Mahuku, George S. Semagn, K. Beyene, Y. Das, B. Makumbi, Dan Kumar, P. Lava Olsen, M. Boddupalli, P.M. |
| author_sort | Nair, S.K. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Abstract Maize streak virus (MSV) disease is a devastating
disease in the Sub-Saharan Africa (SSA), which causes
significant yield loss in maize. Resistance to MSV has
previously been mapped to a major QTL (Msv1) on chromosome
1 that is germplasm and environment independent
and to several minor loci elsewhere in the genome.
In this study, Msv1 was fine-mapped through QTL isogenic
recombinant strategy using a large F2 population of
CML206 × CML312 to an interval of 0.87 cM on chromosome
1. Genome-wide association study was conducted in
the DTMA (Drought Tolerant Maize for Africa)-Association
mapping panel with 278 tropical/sub-tropical breedinglines from CIMMYT using the high-density genotypingby-
sequencing (GBS) markers. This study identified 19
SNPs in the region between 82 and 93 Mb on chromosome
1(B73 RefGen_V2) at a P < 1.00E-04, which coincided
with the fine-mapped region of Msv1. Haplotype trend
regression identified a haplotype block significantly associated
with response to MSV. Three SNPs in this haplotype
block at 87 Mb on chromosome 1 had an accuracy of 0.94
in predicting the disease reaction in a collection of breeding
lines with known responses to MSV infection. In two
biparental populations, selection for resistant Msv1 haplotype
demonstrated a reduction of 1.03–1.39 units on a rating
scale of 1–5, compared to the susceptible haplotype.
High-throughput KASP assays have been developed for
these three SNPs to enable routine marker screening in the
breeding pipeline for MSV resistanceAbstract Maize streak virus (MSV) disease is a devastating
disease in the Sub-Saharan Africa (SSA), which causes
significant yield loss in maize. Resistance to MSV has
previously been mapped to a major QTL (Msv1) on chromosome
1 that is germplasm and environment independent
and to several minor loci elsewhere in the genome.
In this study, Msv1 was fine-mapped through QTL isogenic
recombinant strategy using a large F2 population of
CML206 × CML312 to an interval of 0.87 cM on chromosome
1. Genome-wide association study was conducted in
the DTMA (Drought Tolerant Maize for Africa)-Association
mapping panel with 278 tropical/sub-tropical breedinglines from CIMMYT using the high-density genotypingby-
sequencing (GBS) markers. This study identified 19
SNPs in the region between 82 and 93 Mb on chromosome
1(B73 RefGen_V2) at a P < 1.00E-04, which coincided
with the fine-mapped region of Msv1. Haplotype trend
regression identified a haplotype block significantly associated
with response to MSV. Three SNPs in this haplotype
block at 87 Mb on chromosome 1 had an accuracy of 0.94
in predicting the disease reaction in a collection of breeding
lines with known responses to MSV infection. In two
biparental populations, selection for resistant Msv1 haplotype
demonstrated a reduction of 1.03–1.39 units on a rating
scale of 1–5, compared to the susceptible haplotype.
High-throughput KASP assays have been developed for
these three SNPs to enable routine marker screening in the
breeding pipeline for MSV resistance |
| format | Journal Article |
| id | CGSpace74431 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace744312024-08-27T10:35:25Z Fine mapping of Msv1, a major QTL for resistance to Maize Streak Virus leads to development of production markers for breeding pipelines Nair, S.K. Babu, R. Magorokosho, Cosmos Mahuku, George S. Semagn, K. Beyene, Y. Das, B. Makumbi, Dan Kumar, P. Lava Olsen, M. Boddupalli, P.M. maize streak virus Abstract Maize streak virus (MSV) disease is a devastating disease in the Sub-Saharan Africa (SSA), which causes significant yield loss in maize. Resistance to MSV has previously been mapped to a major QTL (Msv1) on chromosome 1 that is germplasm and environment independent and to several minor loci elsewhere in the genome. In this study, Msv1 was fine-mapped through QTL isogenic recombinant strategy using a large F2 population of CML206 × CML312 to an interval of 0.87 cM on chromosome 1. Genome-wide association study was conducted in the DTMA (Drought Tolerant Maize for Africa)-Association mapping panel with 278 tropical/sub-tropical breedinglines from CIMMYT using the high-density genotypingby- sequencing (GBS) markers. This study identified 19 SNPs in the region between 82 and 93 Mb on chromosome 1(B73 RefGen_V2) at a P < 1.00E-04, which coincided with the fine-mapped region of Msv1. Haplotype trend regression identified a haplotype block significantly associated with response to MSV. Three SNPs in this haplotype block at 87 Mb on chromosome 1 had an accuracy of 0.94 in predicting the disease reaction in a collection of breeding lines with known responses to MSV infection. In two biparental populations, selection for resistant Msv1 haplotype demonstrated a reduction of 1.03–1.39 units on a rating scale of 1–5, compared to the susceptible haplotype. High-throughput KASP assays have been developed for these three SNPs to enable routine marker screening in the breeding pipeline for MSV resistanceAbstract Maize streak virus (MSV) disease is a devastating disease in the Sub-Saharan Africa (SSA), which causes significant yield loss in maize. Resistance to MSV has previously been mapped to a major QTL (Msv1) on chromosome 1 that is germplasm and environment independent and to several minor loci elsewhere in the genome. In this study, Msv1 was fine-mapped through QTL isogenic recombinant strategy using a large F2 population of CML206 × CML312 to an interval of 0.87 cM on chromosome 1. Genome-wide association study was conducted in the DTMA (Drought Tolerant Maize for Africa)-Association mapping panel with 278 tropical/sub-tropical breedinglines from CIMMYT using the high-density genotypingby- sequencing (GBS) markers. This study identified 19 SNPs in the region between 82 and 93 Mb on chromosome 1(B73 RefGen_V2) at a P < 1.00E-04, which coincided with the fine-mapped region of Msv1. Haplotype trend regression identified a haplotype block significantly associated with response to MSV. Three SNPs in this haplotype block at 87 Mb on chromosome 1 had an accuracy of 0.94 in predicting the disease reaction in a collection of breeding lines with known responses to MSV infection. In two biparental populations, selection for resistant Msv1 haplotype demonstrated a reduction of 1.03–1.39 units on a rating scale of 1–5, compared to the susceptible haplotype. High-throughput KASP assays have been developed for these three SNPs to enable routine marker screening in the breeding pipeline for MSV resistance 2015-09 2016-05-25T11:59:23Z 2016-05-25T11:59:23Z Journal Article https://hdl.handle.net/10568/74431 en Limited Access Springer Nair, S.K., Babu, R., Magorokosho, C., Mahuku, G., Semagn, K., Beyene, Y., ... & Boddupalli, P.M. (2015). Fine mapping of Msv1, a major QTL for resistance to Maize Streak Virus leads to development of production markers for breeding pipelines. Theoretical and Applied Genetics, 128(9), 1839-1854. |
| spellingShingle | maize streak virus Nair, S.K. Babu, R. Magorokosho, Cosmos Mahuku, George S. Semagn, K. Beyene, Y. Das, B. Makumbi, Dan Kumar, P. Lava Olsen, M. Boddupalli, P.M. Fine mapping of Msv1, a major QTL for resistance to Maize Streak Virus leads to development of production markers for breeding pipelines |
| title | Fine mapping of Msv1, a major QTL for resistance to Maize Streak Virus leads to development of production markers for breeding pipelines |
| title_full | Fine mapping of Msv1, a major QTL for resistance to Maize Streak Virus leads to development of production markers for breeding pipelines |
| title_fullStr | Fine mapping of Msv1, a major QTL for resistance to Maize Streak Virus leads to development of production markers for breeding pipelines |
| title_full_unstemmed | Fine mapping of Msv1, a major QTL for resistance to Maize Streak Virus leads to development of production markers for breeding pipelines |
| title_short | Fine mapping of Msv1, a major QTL for resistance to Maize Streak Virus leads to development of production markers for breeding pipelines |
| title_sort | fine mapping of msv1 a major qtl for resistance to maize streak virus leads to development of production markers for breeding pipelines |
| topic | maize streak virus |
| url | https://hdl.handle.net/10568/74431 |
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