Incidence, distribution and first identification of citrus tristeza virus by RTPCR in citrus orchards in southWestern Nigeria
In order to ascertain the presence, incidence, and distribution of Citrus tristeza virus (CTV), extensive surveys were conducted in citrus growing areas of south-western Nigeria during 2011-12. A total of 565 citrus trees were sampled in three states. Leaf samples were collected and symptom intensit...
| Main Authors: | , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
International Society for Horticultural Science
2015
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/74430 |
| _version_ | 1855514233440043008 |
|---|---|
| author | Adediji, A.O. Atiri, G.I. Kumar, P. Lava |
| author_browse | Adediji, A.O. Atiri, G.I. Kumar, P. Lava |
| author_facet | Adediji, A.O. Atiri, G.I. Kumar, P. Lava |
| author_sort | Adediji, A.O. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | In order to ascertain the presence, incidence, and distribution of Citrus
tristeza virus (CTV), extensive surveys were conducted in citrus growing areas of
south-western Nigeria during 2011-12. A total of 565 citrus trees were sampled in
three states. Leaf samples were collected and symptom intensity of the trees was
recorded. Symptoms observed included stem pitting, stunting, vein clearing and leaf
curling. Nucleic acid extracts from leaves were used to detect CTV by RT-PCR
using primers PIN1 and PIN2 directed to the conserved 3’ untranslated region.
Results show the presence of CTV in the three states surveyed while symptom
severity differed from one location to another. Disease incidence varied from 85.2%
in Oyo State to 48.4% in Ogun State. Citrus trees were infected regardless of scionrootstock
combinations and the virus was detected in sweet orange (87.0%),
tangerine (77.6%), grapefruit (73.8%) and lemon (69.5%). This is the first report of
molecular detection of CTV in Nigeria.In order to ascertain the presence, incidence, and distribution of Citrus
tristeza virus (CTV), extensive surveys were conducted in citrus growing areas of
south-western Nigeria during 2011-12. A total of 565 citrus trees were sampled in
three states. Leaf samples were collected and symptom intensity of the trees was
recorded. Symptoms observed included stem pitting, stunting, vein clearing and leaf
curling. Nucleic acid extracts from leaves were used to detect CTV by RT-PCR
using primers PIN1 and PIN2 directed to the conserved 3’ untranslated region.
Results show the presence of CTV in the three states surveyed while symptom
severity differed from one location to another. Disease incidence varied from 85.2%
in Oyo State to 48.4% in Ogun State. Citrus trees were infected regardless of scionrootstock
combinations and the virus was detected in sweet orange (87.0%),
tangerine (77.6%), grapefruit (73.8%) and lemon (69.5%). This is the first report of
molecular detection of CTV in Nigeria. |
| format | Journal Article |
| id | CGSpace74430 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | International Society for Horticultural Science |
| publisherStr | International Society for Horticultural Science |
| record_format | dspace |
| spelling | CGSpace744302024-04-25T06:00:15Z Incidence, distribution and first identification of citrus tristeza virus by RTPCR in citrus orchards in southWestern Nigeria Adediji, A.O. Atiri, G.I. Kumar, P. Lava disease surveys symptoms citrus horticulture In order to ascertain the presence, incidence, and distribution of Citrus tristeza virus (CTV), extensive surveys were conducted in citrus growing areas of south-western Nigeria during 2011-12. A total of 565 citrus trees were sampled in three states. Leaf samples were collected and symptom intensity of the trees was recorded. Symptoms observed included stem pitting, stunting, vein clearing and leaf curling. Nucleic acid extracts from leaves were used to detect CTV by RT-PCR using primers PIN1 and PIN2 directed to the conserved 3’ untranslated region. Results show the presence of CTV in the three states surveyed while symptom severity differed from one location to another. Disease incidence varied from 85.2% in Oyo State to 48.4% in Ogun State. Citrus trees were infected regardless of scionrootstock combinations and the virus was detected in sweet orange (87.0%), tangerine (77.6%), grapefruit (73.8%) and lemon (69.5%). This is the first report of molecular detection of CTV in Nigeria.In order to ascertain the presence, incidence, and distribution of Citrus tristeza virus (CTV), extensive surveys were conducted in citrus growing areas of south-western Nigeria during 2011-12. A total of 565 citrus trees were sampled in three states. Leaf samples were collected and symptom intensity of the trees was recorded. Symptoms observed included stem pitting, stunting, vein clearing and leaf curling. Nucleic acid extracts from leaves were used to detect CTV by RT-PCR using primers PIN1 and PIN2 directed to the conserved 3’ untranslated region. Results show the presence of CTV in the three states surveyed while symptom severity differed from one location to another. Disease incidence varied from 85.2% in Oyo State to 48.4% in Ogun State. Citrus trees were infected regardless of scionrootstock combinations and the virus was detected in sweet orange (87.0%), tangerine (77.6%), grapefruit (73.8%) and lemon (69.5%). This is the first report of molecular detection of CTV in Nigeria. 2015-01 2016-05-25T11:59:23Z 2016-05-25T11:59:23Z Journal Article https://hdl.handle.net/10568/74430 en Limited Access International Society for Horticultural Science Adediji, A.O., Atiri, G.I., & Kumar, P.L. (2015). Incidence, distribution and first identification of citrus tristeza virus by RT-PCR in citrus orchards in South-Western Nigeria. Acta Horticulturae, 12(1065), 759-766. |
| spellingShingle | disease surveys symptoms citrus horticulture Adediji, A.O. Atiri, G.I. Kumar, P. Lava Incidence, distribution and first identification of citrus tristeza virus by RTPCR in citrus orchards in southWestern Nigeria |
| title | Incidence, distribution and first identification of citrus tristeza virus by RTPCR in citrus orchards in southWestern Nigeria |
| title_full | Incidence, distribution and first identification of citrus tristeza virus by RTPCR in citrus orchards in southWestern Nigeria |
| title_fullStr | Incidence, distribution and first identification of citrus tristeza virus by RTPCR in citrus orchards in southWestern Nigeria |
| title_full_unstemmed | Incidence, distribution and first identification of citrus tristeza virus by RTPCR in citrus orchards in southWestern Nigeria |
| title_short | Incidence, distribution and first identification of citrus tristeza virus by RTPCR in citrus orchards in southWestern Nigeria |
| title_sort | incidence distribution and first identification of citrus tristeza virus by rtpcr in citrus orchards in southwestern nigeria |
| topic | disease surveys symptoms citrus horticulture |
| url | https://hdl.handle.net/10568/74430 |
| work_keys_str_mv | AT adedijiao incidencedistributionandfirstidentificationofcitrustristezavirusbyrtpcrincitrusorchardsinsouthwesternnigeria AT atirigi incidencedistributionandfirstidentificationofcitrustristezavirusbyrtpcrincitrusorchardsinsouthwesternnigeria AT kumarplava incidencedistributionandfirstidentificationofcitrustristezavirusbyrtpcrincitrusorchardsinsouthwesternnigeria |