Analysis of microbial taxonomical groups present in maize stalks suppressive to colonization by toxigenic Fusarium spp.: a strategy for the identification of potential antagonists

Pink ear rot of maize caused by Fusarium verticillioides, Fusarium proliferatum and Fusarium graminearum can lead to severe yield losses and contamination of grain with a range of mycotoxins. Maize stalks colonized by Fusarium spp. are the main primary inoculum source for Fusarium incited epidemics...

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Main Authors: Köhl, J., Lombaers, C., Moretti, A., Bandyopadhyay, Ranajit, Somma, S., Kastelein, P.
Format: Journal Article
Language:Inglés
Published: Elsevier 2015
Subjects:
Online Access:https://hdl.handle.net/10568/74419
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author Köhl, J.
Lombaers, C.
Moretti, A.
Bandyopadhyay, Ranajit
Somma, S.
Kastelein, P.
author_browse Bandyopadhyay, Ranajit
Kastelein, P.
Köhl, J.
Lombaers, C.
Moretti, A.
Somma, S.
author_facet Köhl, J.
Lombaers, C.
Moretti, A.
Bandyopadhyay, Ranajit
Somma, S.
Kastelein, P.
author_sort Köhl, J.
collection Repository of Agricultural Research Outputs (CGSpace)
description Pink ear rot of maize caused by Fusarium verticillioides, Fusarium proliferatum and Fusarium graminearum can lead to severe yield losses and contamination of grain with a range of mycotoxins. Maize stalks colonized by Fusarium spp. are the main primary inoculum source for Fusarium incited epidemics in maize or other susceptible crops grown in rotation. The colonization of individual maize stalks originating from fields in The Netherlands, Italy and Nigeria by Fusarium spp. was quantified using specific TaqMan-PCR assays. Highest values were found for F. graminearum and Fusarium avenaceum in Dutch samples, for F. graminearum and FUM producing species (F. verticillioides and F. proliferatum) in Italian samples and FUM producing Fusarium spp. in Nigerian samples. The increase in Fusarium spp. colonization under field conditions during a period of 3–6 months after harvest of the maize crops varied considerably between individual stalks. The fungal and bacterial diversity was analyzed for sub-sets of stalks with high versus low increase of Fusarium colonization. Bacterial taxonomic groups such as Bacillus, Curtobacterium, Erwinia, Flavobacterium, Novosphingobium, Pantoea, Sphingomonas, Rahnella and Staphylococcus and fungal taxonomic groups such as Acremomium sp., Cryptococcus flavescens, Cryptococcus zeae, Leptosphaeria sp. and Microdochium bolleyi were more abundant in the stalks with lower increase in pathogen colonization. Such fungal and bacterial groups associated with ‘suppressive stalks’ may be antagonistic to Fusarium spp. and a source of candidate strains for the selection of new biological control agents.Pink ear rot of maize caused by Fusarium verticillioides, Fusarium proliferatum and Fusarium graminearum can lead to severe yield losses and contamination of grain with a range of mycotoxins. Maize stalks colonized by Fusarium spp. are the main primary inoculum source for Fusarium incited epidemics in maize or other susceptible crops grown in rotation. The colonization of individual maize stalks originating from fields in The Netherlands, Italy and Nigeria by Fusarium spp. was quantified using specific TaqMan-PCR assays. Highest values were found for F. graminearum and Fusarium avenaceum in Dutch samples, for F. graminearum and FUM producing species (F. verticillioides and F. proliferatum) in Italian samples and FUM producing Fusarium spp. in Nigerian samples. The increase in Fusarium spp. colonization under field conditions during a period of 3–6 months after harvest of the maize crops varied considerably between individual stalks. The fungal and bacterial diversity was analyzed for sub-sets of stalks with high versus low increase of Fusarium colonization. Bacterial taxonomic groups such as Bacillus, Curtobacterium, Erwinia, Flavobacterium, Novosphingobium, Pantoea, Sphingomonas, Rahnella and Staphylococcus and fungal taxonomic groups such as Acremomium sp., Cryptococcus flavescens, Cryptococcus zeae, Leptosphaeria sp. and Microdochium bolleyi were more abundant in the stalks with lower increase in pathogen colonization. Such fungal and bacterial groups associated with ‘suppressive stalks’ may be antagonistic to Fusarium spp. and a source of candidate strains for the selection of new biological control agents.
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spelling CGSpace744192023-12-08T19:36:04Z Analysis of microbial taxonomical groups present in maize stalks suppressive to colonization by toxigenic Fusarium spp.: a strategy for the identification of potential antagonists Köhl, J. Lombaers, C. Moretti, A. Bandyopadhyay, Ranajit Somma, S. Kastelein, P. crop residues biological control maize fusarium Pink ear rot of maize caused by Fusarium verticillioides, Fusarium proliferatum and Fusarium graminearum can lead to severe yield losses and contamination of grain with a range of mycotoxins. Maize stalks colonized by Fusarium spp. are the main primary inoculum source for Fusarium incited epidemics in maize or other susceptible crops grown in rotation. The colonization of individual maize stalks originating from fields in The Netherlands, Italy and Nigeria by Fusarium spp. was quantified using specific TaqMan-PCR assays. Highest values were found for F. graminearum and Fusarium avenaceum in Dutch samples, for F. graminearum and FUM producing species (F. verticillioides and F. proliferatum) in Italian samples and FUM producing Fusarium spp. in Nigerian samples. The increase in Fusarium spp. colonization under field conditions during a period of 3–6 months after harvest of the maize crops varied considerably between individual stalks. The fungal and bacterial diversity was analyzed for sub-sets of stalks with high versus low increase of Fusarium colonization. Bacterial taxonomic groups such as Bacillus, Curtobacterium, Erwinia, Flavobacterium, Novosphingobium, Pantoea, Sphingomonas, Rahnella and Staphylococcus and fungal taxonomic groups such as Acremomium sp., Cryptococcus flavescens, Cryptococcus zeae, Leptosphaeria sp. and Microdochium bolleyi were more abundant in the stalks with lower increase in pathogen colonization. Such fungal and bacterial groups associated with ‘suppressive stalks’ may be antagonistic to Fusarium spp. and a source of candidate strains for the selection of new biological control agents.Pink ear rot of maize caused by Fusarium verticillioides, Fusarium proliferatum and Fusarium graminearum can lead to severe yield losses and contamination of grain with a range of mycotoxins. Maize stalks colonized by Fusarium spp. are the main primary inoculum source for Fusarium incited epidemics in maize or other susceptible crops grown in rotation. The colonization of individual maize stalks originating from fields in The Netherlands, Italy and Nigeria by Fusarium spp. was quantified using specific TaqMan-PCR assays. Highest values were found for F. graminearum and Fusarium avenaceum in Dutch samples, for F. graminearum and FUM producing species (F. verticillioides and F. proliferatum) in Italian samples and FUM producing Fusarium spp. in Nigerian samples. The increase in Fusarium spp. colonization under field conditions during a period of 3–6 months after harvest of the maize crops varied considerably between individual stalks. The fungal and bacterial diversity was analyzed for sub-sets of stalks with high versus low increase of Fusarium colonization. Bacterial taxonomic groups such as Bacillus, Curtobacterium, Erwinia, Flavobacterium, Novosphingobium, Pantoea, Sphingomonas, Rahnella and Staphylococcus and fungal taxonomic groups such as Acremomium sp., Cryptococcus flavescens, Cryptococcus zeae, Leptosphaeria sp. and Microdochium bolleyi were more abundant in the stalks with lower increase in pathogen colonization. Such fungal and bacterial groups associated with ‘suppressive stalks’ may be antagonistic to Fusarium spp. and a source of candidate strains for the selection of new biological control agents. 2015-04 2016-05-25T11:59:03Z 2016-05-25T11:59:03Z Journal Article https://hdl.handle.net/10568/74419 en Limited Access Elsevier Köhl, J., Lombaers, C., Moretti, A., Bandyopadhyay, R., Somma, S., & Kastelein, P. (2015). Analysis of microbial taxonomical groups present in maize stalks suppressive to colonization by toxigenic Fusarium spp.: a strategy for the identification of potential antagonists. Biological Control, 83, 20-28.
spellingShingle crop residues
biological control
maize
fusarium
Köhl, J.
Lombaers, C.
Moretti, A.
Bandyopadhyay, Ranajit
Somma, S.
Kastelein, P.
Analysis of microbial taxonomical groups present in maize stalks suppressive to colonization by toxigenic Fusarium spp.: a strategy for the identification of potential antagonists
title Analysis of microbial taxonomical groups present in maize stalks suppressive to colonization by toxigenic Fusarium spp.: a strategy for the identification of potential antagonists
title_full Analysis of microbial taxonomical groups present in maize stalks suppressive to colonization by toxigenic Fusarium spp.: a strategy for the identification of potential antagonists
title_fullStr Analysis of microbial taxonomical groups present in maize stalks suppressive to colonization by toxigenic Fusarium spp.: a strategy for the identification of potential antagonists
title_full_unstemmed Analysis of microbial taxonomical groups present in maize stalks suppressive to colonization by toxigenic Fusarium spp.: a strategy for the identification of potential antagonists
title_short Analysis of microbial taxonomical groups present in maize stalks suppressive to colonization by toxigenic Fusarium spp.: a strategy for the identification of potential antagonists
title_sort analysis of microbial taxonomical groups present in maize stalks suppressive to colonization by toxigenic fusarium spp a strategy for the identification of potential antagonists
topic crop residues
biological control
maize
fusarium
url https://hdl.handle.net/10568/74419
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