A metabolomics approach to the assessment of banana diversity and traits

Banana (Musa) is one of the most important economic and staple crops in the world. The majority of edible cultivated banana species arises from two species of the Eumusa group, Musa acuminata (A genome) and Musa balbisiana(B genome). In order to assess the biochemical diversity that exists in our ba...

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Autores principales: Drapal, Margit, Carvalho, E., Houwe, Ines van den, Rouard, M., Sardos, J., Amah, D., Swennen, Rony L., Roux, N., Fraser, P.
Formato: Conference Paper
Lenguaje:Inglés
Publicado: 2016
Materias:
Acceso en línea:https://hdl.handle.net/10568/73458
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author Drapal, Margit
Carvalho, E.
Houwe, Ines van den
Rouard, M.
Sardos, J.
Amah, D.
Swennen, Rony L.
Roux, N.
Fraser, P.
author_browse Amah, D.
Carvalho, E.
Drapal, Margit
Fraser, P.
Houwe, Ines van den
Rouard, M.
Roux, N.
Sardos, J.
Swennen, Rony L.
author_facet Drapal, Margit
Carvalho, E.
Houwe, Ines van den
Rouard, M.
Sardos, J.
Amah, D.
Swennen, Rony L.
Roux, N.
Fraser, P.
author_sort Drapal, Margit
collection Repository of Agricultural Research Outputs (CGSpace)
description Banana (Musa) is one of the most important economic and staple crops in the world. The majority of edible cultivated banana species arises from two species of the Eumusa group, Musa acuminata (A genome) and Musa balbisiana(B genome). In order to assess the biochemical diversity that exists in our banana germplasm collections multi-platform metabolomics platforms has been established for banana. These include LC-MS in untargeted and targeted mode, GC-MS based metabolite profiling and targeted UPLC-PDA for compounds such as carotenoids where MS ionisation is poor. Metabolomic finger printing and complementary targeted analysis has been performed on in vitro vegetative material for 20 diverse Musa accessions, including diploid varieties, wild Musa acuminata and Musa balbisiana as well as different triploids and distant wild species (Musa ornata). The data allowed the separation of the genotypes on the basis of genotypes and differentiating metabolites identified between accessions. Comparisons with field grown material was carried out in selected cases and clear correlation was observed including the potential to predict fruit phenotypes on vegetative profiles. These robust techniques can now be utilised in combination with of omic approaches to characterise consumer and agronomic traits within breeding populations.
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spelling CGSpace734582024-01-17T12:58:34Z A metabolomics approach to the assessment of banana diversity and traits Drapal, Margit Carvalho, E. Houwe, Ines van den Rouard, M. Sardos, J. Amah, D. Swennen, Rony L. Roux, N. Fraser, P. musa genetic variation genotypes metabolites Banana (Musa) is one of the most important economic and staple crops in the world. The majority of edible cultivated banana species arises from two species of the Eumusa group, Musa acuminata (A genome) and Musa balbisiana(B genome). In order to assess the biochemical diversity that exists in our banana germplasm collections multi-platform metabolomics platforms has been established for banana. These include LC-MS in untargeted and targeted mode, GC-MS based metabolite profiling and targeted UPLC-PDA for compounds such as carotenoids where MS ionisation is poor. Metabolomic finger printing and complementary targeted analysis has been performed on in vitro vegetative material for 20 diverse Musa accessions, including diploid varieties, wild Musa acuminata and Musa balbisiana as well as different triploids and distant wild species (Musa ornata). The data allowed the separation of the genotypes on the basis of genotypes and differentiating metabolites identified between accessions. Comparisons with field grown material was carried out in selected cases and clear correlation was observed including the potential to predict fruit phenotypes on vegetative profiles. These robust techniques can now be utilised in combination with of omic approaches to characterise consumer and agronomic traits within breeding populations. 2016 2016-05-20T10:27:50Z 2016-05-20T10:27:50Z Conference Paper https://hdl.handle.net/10568/73458 en Open Access Drapal, M.; Carvalho, E.; van den Houwe, I.; Rouard, M.; Sardos, J.; Amah, D.; Swennen, R.; Roux, N.; Fraser, P. (2016) A metabolomics approach to the assessment of banana diversity and traits. [Abstract] presented at XXIV Plant and Animal Genome Conference. San Diego, CA (USA) 9-13 Jan 2016.
spellingShingle musa
genetic variation
genotypes
metabolites
Drapal, Margit
Carvalho, E.
Houwe, Ines van den
Rouard, M.
Sardos, J.
Amah, D.
Swennen, Rony L.
Roux, N.
Fraser, P.
A metabolomics approach to the assessment of banana diversity and traits
title A metabolomics approach to the assessment of banana diversity and traits
title_full A metabolomics approach to the assessment of banana diversity and traits
title_fullStr A metabolomics approach to the assessment of banana diversity and traits
title_full_unstemmed A metabolomics approach to the assessment of banana diversity and traits
title_short A metabolomics approach to the assessment of banana diversity and traits
title_sort metabolomics approach to the assessment of banana diversity and traits
topic musa
genetic variation
genotypes
metabolites
url https://hdl.handle.net/10568/73458
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