Two Novel DNAs that enhance symptoms and overcome CMD2 resistance to cassava mosaic disease
Cassava mosaic begomoviruses (CMBs) cause cassava mosaic disease (CMD) across Africa and the Indian subcontinent. Like all members of the geminivirus family, CMBs have small, circular single-stranded DNA genomes. We report here the discovery of two novel DNA sequences, designated SEGS-1 and SEGS-2 (...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
American Society for Microbiology
2016
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/73456 |
| _version_ | 1855530558296162304 |
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| author | Ndunguru, Joseph León, Leandro de Doyle, Catherine D. Sseruwagi, Peter Plata, Germán Legg, James P. Thompson, Graham Tohme, Joseph M. Aveling, Theresa Ascencio Ibáñez, Jose T. Hanley Bowdoin, Linda |
| author_browse | Ascencio Ibáñez, Jose T. Aveling, Theresa Doyle, Catherine D. Hanley Bowdoin, Linda Legg, James P. León, Leandro de Ndunguru, Joseph Plata, Germán Sseruwagi, Peter Thompson, Graham Tohme, Joseph M. |
| author_facet | Ndunguru, Joseph León, Leandro de Doyle, Catherine D. Sseruwagi, Peter Plata, Germán Legg, James P. Thompson, Graham Tohme, Joseph M. Aveling, Theresa Ascencio Ibáñez, Jose T. Hanley Bowdoin, Linda |
| author_sort | Ndunguru, Joseph |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava mosaic begomoviruses (CMBs) cause cassava mosaic disease (CMD) across Africa and the Indian subcontinent. Like all members of the geminivirus family, CMBs have small, circular single-stranded DNA genomes. We report here the discovery of two novel DNA sequences, designated SEGS-1 and SEGS-2 (for sequences enhancing geminivirus symptoms), that enhance symptoms and break resistance to CMD. The SEGS are characterized by GC-rich regions and the absence of long open reading frames. Both SEGS enhanced CMD symptoms in cassava (Manihot esculenta Crantz) when coinoculated with African cassava mosaic virus (ACMV), East African cassava mosaic Cameroon virus (EACMCV), or East African cassava mosaic virus-Uganda (EACMV-UG). SEGS-1 also overcame resistance of a cassava landrace carrying the CMD2 resistance locus when coinoculated with EACMV-UG. Episomal forms of both SEGS were detected in CMB-infected cassava but not in healthy cassava. SEGS-2 episomes were also found in virions and whiteflies. SEGS-1 has no homology to geminiviruses or their associated satellites, but the cassava genome contains a sequence that is 99% identical to full-length SEGS-1. The cassava genome also includes three sequences with 84 to 89% identity to SEGS-2 that together encompass all of SEGS-2 except for a 52-bp region, which includes the episomal junction and a 26-bp sequence related to alphasatellite replication origins. These results suggest that SEGS-1 is derived from the cassava genome and facilitates CMB infection as an integrated copy and/or an episome, while SEGS-2 was originally from the cassava genome but now is encapsidated into virions and transmitted as an episome by whiteflies. |
| format | Journal Article |
| id | CGSpace73456 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | American Society for Microbiology |
| publisherStr | American Society for Microbiology |
| record_format | dspace |
| spelling | CGSpace734562025-03-13T09:44:36Z Two Novel DNAs that enhance symptoms and overcome CMD2 resistance to cassava mosaic disease Ndunguru, Joseph León, Leandro de Doyle, Catherine D. Sseruwagi, Peter Plata, Germán Legg, James P. Thompson, Graham Tohme, Joseph M. Aveling, Theresa Ascencio Ibáñez, Jose T. Hanley Bowdoin, Linda manihot esculenta african cassava mosaic virus plant diseases whiteflies disease resistance genetic resistance plant breeding virus del mosaico de la cassava enfermedades de las plantas resistencia a la enfermedad resistencia genética fitomejoramiento Cassava mosaic begomoviruses (CMBs) cause cassava mosaic disease (CMD) across Africa and the Indian subcontinent. Like all members of the geminivirus family, CMBs have small, circular single-stranded DNA genomes. We report here the discovery of two novel DNA sequences, designated SEGS-1 and SEGS-2 (for sequences enhancing geminivirus symptoms), that enhance symptoms and break resistance to CMD. The SEGS are characterized by GC-rich regions and the absence of long open reading frames. Both SEGS enhanced CMD symptoms in cassava (Manihot esculenta Crantz) when coinoculated with African cassava mosaic virus (ACMV), East African cassava mosaic Cameroon virus (EACMCV), or East African cassava mosaic virus-Uganda (EACMV-UG). SEGS-1 also overcame resistance of a cassava landrace carrying the CMD2 resistance locus when coinoculated with EACMV-UG. Episomal forms of both SEGS were detected in CMB-infected cassava but not in healthy cassava. SEGS-2 episomes were also found in virions and whiteflies. SEGS-1 has no homology to geminiviruses or their associated satellites, but the cassava genome contains a sequence that is 99% identical to full-length SEGS-1. The cassava genome also includes three sequences with 84 to 89% identity to SEGS-2 that together encompass all of SEGS-2 except for a 52-bp region, which includes the episomal junction and a 26-bp sequence related to alphasatellite replication origins. These results suggest that SEGS-1 is derived from the cassava genome and facilitates CMB infection as an integrated copy and/or an episome, while SEGS-2 was originally from the cassava genome but now is encapsidated into virions and transmitted as an episome by whiteflies. 2016-04-15 2016-05-19T20:34:19Z 2016-05-19T20:34:19Z Journal Article https://hdl.handle.net/10568/73456 en Open Access American Society for Microbiology Ndunguru, Joseph; De León, Leandro; Doyle, Catherine D.; Sseruwagi, Peter; Plata, German; Legg, James P.; Thompson, Graham; Tohme, Joseph M.; Aveling, Theresa; Ascencio Ibáñez, Jose T.; Hanley Bowdoin, Linda. 2016. Two Novel DNAs that enhance symptoms and overcome CMD2 resistance to cassava mosaic disease . Journal of Virology 90(8): 4160-4173. |
| spellingShingle | manihot esculenta african cassava mosaic virus plant diseases whiteflies disease resistance genetic resistance plant breeding virus del mosaico de la cassava enfermedades de las plantas resistencia a la enfermedad resistencia genética fitomejoramiento Ndunguru, Joseph León, Leandro de Doyle, Catherine D. Sseruwagi, Peter Plata, Germán Legg, James P. Thompson, Graham Tohme, Joseph M. Aveling, Theresa Ascencio Ibáñez, Jose T. Hanley Bowdoin, Linda Two Novel DNAs that enhance symptoms and overcome CMD2 resistance to cassava mosaic disease |
| title | Two Novel DNAs that enhance symptoms and overcome CMD2 resistance to cassava mosaic disease |
| title_full | Two Novel DNAs that enhance symptoms and overcome CMD2 resistance to cassava mosaic disease |
| title_fullStr | Two Novel DNAs that enhance symptoms and overcome CMD2 resistance to cassava mosaic disease |
| title_full_unstemmed | Two Novel DNAs that enhance symptoms and overcome CMD2 resistance to cassava mosaic disease |
| title_short | Two Novel DNAs that enhance symptoms and overcome CMD2 resistance to cassava mosaic disease |
| title_sort | two novel dnas that enhance symptoms and overcome cmd2 resistance to cassava mosaic disease |
| topic | manihot esculenta african cassava mosaic virus plant diseases whiteflies disease resistance genetic resistance plant breeding virus del mosaico de la cassava enfermedades de las plantas resistencia a la enfermedad resistencia genética fitomejoramiento |
| url | https://hdl.handle.net/10568/73456 |
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