Limited genetic and antigenic diversity within parasite isolates used in a live vaccine against Theileria parva
An infection and treatment protocol is used to vaccinate cattle against *Theileria parva* infection. Due to incomplete cross-protection between different parasite isolates, a mixture of three isolates, termed the Muguga cocktail, is used for vaccination. While vaccination of cattle in some regions p...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Elsevier
2016
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/73303 |
| _version_ | 1855513380001939456 |
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| author | Hemmink, Johanneke D. Weir, W. MacHugh, Niall D. Graham, S.P. Patel, Ekta Paxton, E. Shiels, B. Toye, Philip G. Morrison, W.I. Pelle, Roger |
| author_browse | Graham, S.P. Hemmink, Johanneke D. MacHugh, Niall D. Morrison, W.I. Patel, Ekta Paxton, E. Pelle, Roger Shiels, B. Toye, Philip G. Weir, W. |
| author_facet | Hemmink, Johanneke D. Weir, W. MacHugh, Niall D. Graham, S.P. Patel, Ekta Paxton, E. Shiels, B. Toye, Philip G. Morrison, W.I. Pelle, Roger |
| author_sort | Hemmink, Johanneke D. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | An infection and treatment protocol is used to vaccinate cattle against *Theileria parva* infection. Due to incomplete cross-protection between different parasite isolates, a mixture of three isolates, termed the Muguga cocktail, is used for vaccination. While vaccination of cattle in some regions provides high levels of protection, some animals are not protected against challenge with buffalo-derived *T. parva*. Knowledge of the genetic composition of the Muguga cocktail vaccine is required to understand how vaccination is able to protect against field challenge and to identify the potential limitations of the vaccine. The aim of the current study was to determine the extent of genetic and antigenic diversity within the parasite isolates that constitute the Muguga cocktail. High throughput multi-locus sequencing of antigen-encoding loci was performed in parallel with typing using a panel of micro- and mini-satellite loci. The former focused on genes encoding CD8+ T cell antigens, believed to be relevant to protective immunity. The results demonstrate that each of the three component stocks of the cocktail contains limited parasite genotypic diversity, with single alleles detected at many gene/satellite loci and, moreover, that two of the components show a very high level of similarity. Thus, the vaccine incorporates very little of the genetic and antigenic diversity observed in field populations of *T. parva*. The presence of alleles at low frequency (<10%) within vaccine component populations also points to the possibility of variability in the content of vaccine doses and the potential for loss of allelic diversity during tick passage. The results demonstrate that there is scope to modify the content of the vaccine in order to enhance its diversity and thus its potential for providing broad protection. The ability to accurately quantify genetic diversity in vaccine component stocks will facilitate improved quality control procedures designed to ensure the long-term efficacy of the vaccine. |
| format | Journal Article |
| id | CGSpace73303 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace733032024-10-09T10:07:33Z Limited genetic and antigenic diversity within parasite isolates used in a live vaccine against Theileria parva Hemmink, Johanneke D. Weir, W. MacHugh, Niall D. Graham, S.P. Patel, Ekta Paxton, E. Shiels, B. Toye, Philip G. Morrison, W.I. Pelle, Roger animal diseases vaccines research infectious diseases parasitology An infection and treatment protocol is used to vaccinate cattle against *Theileria parva* infection. Due to incomplete cross-protection between different parasite isolates, a mixture of three isolates, termed the Muguga cocktail, is used for vaccination. While vaccination of cattle in some regions provides high levels of protection, some animals are not protected against challenge with buffalo-derived *T. parva*. Knowledge of the genetic composition of the Muguga cocktail vaccine is required to understand how vaccination is able to protect against field challenge and to identify the potential limitations of the vaccine. The aim of the current study was to determine the extent of genetic and antigenic diversity within the parasite isolates that constitute the Muguga cocktail. High throughput multi-locus sequencing of antigen-encoding loci was performed in parallel with typing using a panel of micro- and mini-satellite loci. The former focused on genes encoding CD8+ T cell antigens, believed to be relevant to protective immunity. The results demonstrate that each of the three component stocks of the cocktail contains limited parasite genotypic diversity, with single alleles detected at many gene/satellite loci and, moreover, that two of the components show a very high level of similarity. Thus, the vaccine incorporates very little of the genetic and antigenic diversity observed in field populations of *T. parva*. The presence of alleles at low frequency (<10%) within vaccine component populations also points to the possibility of variability in the content of vaccine doses and the potential for loss of allelic diversity during tick passage. The results demonstrate that there is scope to modify the content of the vaccine in order to enhance its diversity and thus its potential for providing broad protection. The ability to accurately quantify genetic diversity in vaccine component stocks will facilitate improved quality control procedures designed to ensure the long-term efficacy of the vaccine. 2016-07 2016-05-05T05:43:08Z 2016-05-05T05:43:08Z Journal Article https://hdl.handle.net/10568/73303 en Open Access Elsevier Hemmink, J.D., Weir, W., MacHugh, N.D., Graham, S.P., Patel, E., Paxton, E., Shiels, B., Toye, P.G., Morrison, W.I. and Pelle, R. 2016. Limited genetic and antigenic diversity within parasite isolates used in a live vaccine against Theileria parva. International Journal for Parasitology 46(8):495–506. |
| spellingShingle | animal diseases vaccines research infectious diseases parasitology Hemmink, Johanneke D. Weir, W. MacHugh, Niall D. Graham, S.P. Patel, Ekta Paxton, E. Shiels, B. Toye, Philip G. Morrison, W.I. Pelle, Roger Limited genetic and antigenic diversity within parasite isolates used in a live vaccine against Theileria parva |
| title | Limited genetic and antigenic diversity within parasite isolates used in a live vaccine against Theileria parva |
| title_full | Limited genetic and antigenic diversity within parasite isolates used in a live vaccine against Theileria parva |
| title_fullStr | Limited genetic and antigenic diversity within parasite isolates used in a live vaccine against Theileria parva |
| title_full_unstemmed | Limited genetic and antigenic diversity within parasite isolates used in a live vaccine against Theileria parva |
| title_short | Limited genetic and antigenic diversity within parasite isolates used in a live vaccine against Theileria parva |
| title_sort | limited genetic and antigenic diversity within parasite isolates used in a live vaccine against theileria parva |
| topic | animal diseases vaccines research infectious diseases parasitology |
| url | https://hdl.handle.net/10568/73303 |
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