Potential of Trichoderma asperellum for biocontrol of Fusarium wilt in banana

A total of 72 fungal endophytes were isolated from surface-sterilized roots of healthy 'Gros Michel' (AAA genome, Gros Michel subgroup) banana plants in Turrialba, Costa Rica. In vitro and in vivo tests were carried out in order to identify microorganisms with potential activity against Fusarium oxy...

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Main Authors: Chaves, N.P., Staver, Charles, Dita, M.A.
Format: Conference Paper
Language:Inglés
Published: International Society for Horticultural Science 2016
Subjects:
Online Access:https://hdl.handle.net/10568/73229
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author Chaves, N.P.
Staver, Charles
Dita, M.A.
author_browse Chaves, N.P.
Dita, M.A.
Staver, Charles
author_facet Chaves, N.P.
Staver, Charles
Dita, M.A.
author_sort Chaves, N.P.
collection Repository of Agricultural Research Outputs (CGSpace)
description A total of 72 fungal endophytes were isolated from surface-sterilized roots of healthy 'Gros Michel' (AAA genome, Gros Michel subgroup) banana plants in Turrialba, Costa Rica. In vitro and in vivo tests were carried out in order to identify microorganisms with potential activity against Fusarium oxysporum f. sp. cubense (Foc, race 1). In vitro tests revealed that 22 isolates inhibited the growth of Foc. The four most effective isolates were selected for suppression of Foc under greenhouse conditions. Plants treated with the four isolates showed a significant reduction of external and internal symptoms compared with untreated plants. Based on sequence analyses of specific genome regions [elongation factor - Tefa-1α or Internal Transcribed Spacer - ITS], the fungi tested in the greenhouse were identified as Trichoderma asperellum. Field trials are currently underway in Costa Rica to verify the performance of T. asperellum isolates as potential biological control agents against Foc. Results indicate that certain endophytic microorganisms may play important roles in controlling Fusarium wilt in banana. A better understanding of the antagonistic mechanisms displayed by endophytes in order to provide the best conditions to optimize the antagonistic activities of beneficial strains is still required.
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spelling CGSpace732292024-05-15T05:12:02Z Potential of Trichoderma asperellum for biocontrol of Fusarium wilt in banana Chaves, N.P. Staver, Charles Dita, M.A. endophytes fungal diseases trichoderma biological control A total of 72 fungal endophytes were isolated from surface-sterilized roots of healthy 'Gros Michel' (AAA genome, Gros Michel subgroup) banana plants in Turrialba, Costa Rica. In vitro and in vivo tests were carried out in order to identify microorganisms with potential activity against Fusarium oxysporum f. sp. cubense (Foc, race 1). In vitro tests revealed that 22 isolates inhibited the growth of Foc. The four most effective isolates were selected for suppression of Foc under greenhouse conditions. Plants treated with the four isolates showed a significant reduction of external and internal symptoms compared with untreated plants. Based on sequence analyses of specific genome regions [elongation factor - Tefa-1α or Internal Transcribed Spacer - ITS], the fungi tested in the greenhouse were identified as Trichoderma asperellum. Field trials are currently underway in Costa Rica to verify the performance of T. asperellum isolates as potential biological control agents against Foc. Results indicate that certain endophytic microorganisms may play important roles in controlling Fusarium wilt in banana. A better understanding of the antagonistic mechanisms displayed by endophytes in order to provide the best conditions to optimize the antagonistic activities of beneficial strains is still required. 2016-03 2016-04-28T08:58:23Z 2016-04-28T08:58:23Z Conference Paper https://hdl.handle.net/10568/73229 en https://hdl.handle.net/10568/72946 Limited Access International Society for Horticultural Science Chaves, N.P.; Staver, C.; Dita, M.A. (2016) Potential of Trichoderma asperellum for biocontrol of Fusarium wilt in banana. In: Proceedings. IX International Symposium on Banana: ISHS-ProMusa Symposium on Unravelling the Banana's Genomic Potential. (Smith, M. et al (eds.)) Acta Horticulturae, 1114: p. 261-266. Leuven (Belgium), ISHS. ISBN: 978-94-62611-08-5
spellingShingle endophytes
fungal diseases
trichoderma
biological control
Chaves, N.P.
Staver, Charles
Dita, M.A.
Potential of Trichoderma asperellum for biocontrol of Fusarium wilt in banana
title Potential of Trichoderma asperellum for biocontrol of Fusarium wilt in banana
title_full Potential of Trichoderma asperellum for biocontrol of Fusarium wilt in banana
title_fullStr Potential of Trichoderma asperellum for biocontrol of Fusarium wilt in banana
title_full_unstemmed Potential of Trichoderma asperellum for biocontrol of Fusarium wilt in banana
title_short Potential of Trichoderma asperellum for biocontrol of Fusarium wilt in banana
title_sort potential of trichoderma asperellum for biocontrol of fusarium wilt in banana
topic endophytes
fungal diseases
trichoderma
biological control
url https://hdl.handle.net/10568/73229
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