The field verification activity: a cooperative approach to the management of the global Musa in vitro collection at the International Transit Centre
The International Transit Centre (ITC) (Bioversity International, KULeuven, Belgium) currently holds the largest ex situ collection of Musa germplasm, partly available to researchers, breeders and the larger Musa community via the online ordering system in the Musa Germplasm Information System (MGIS...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Conference Paper |
| Language: | Inglés |
| Published: |
International Society for Horticultural Science
2016
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/73057 |
| _version_ | 1855534477439139840 |
|---|---|
| author | Chase, R. Sardos, J. Ruas, M. Houwe, Ines van den Roux, N. Hribova, E. Doležel, Jaroslav |
| author_browse | Chase, R. Doležel, Jaroslav Houwe, Ines van den Hribova, E. Roux, N. Ruas, M. Sardos, J. |
| author_facet | Chase, R. Sardos, J. Ruas, M. Houwe, Ines van den Roux, N. Hribova, E. Doležel, Jaroslav |
| author_sort | Chase, R. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The International Transit Centre (ITC) (Bioversity International, KULeuven, Belgium) currently holds the largest ex situ collection of Musa germplasm, partly available to researchers, breeders and the larger Musa community via the online ordering system in the Musa Germplasm Information System (MGIS). It is thus extremely important that somaclonal variants and mislabeled accessions are removed from the collection to ensure that users have access to a valid and reliable source of Musa genetic resources. Through Bioversity's Field Verification (FV) activity, a combination of morphological and molecular characterization along with a validation by a panel of experts is used to assess the genetic integrity of the Musa accessions held at ITC. The FV includes ploidy determination and SSR and DArT fingerprinting data (produced by the Musa Genotyping Centre, Institute of Experimental Botany, Czech Republic and DArT P/L, Australia) combined with minimum descriptors and standard photographs of accessions in partners' field collections. The compiled molecular and morphological data are sent to a panel of ten Musa taxonomists in charge of the validation of the accessions' genetic integrity. This workflow allows the quality control of the germplasm available to users while adding value to the content of the ITC by producing standardized characterization data. Status has been determined for 370 of 832 accessions sent to field partners, and the first round will be completed when all virus-free accessions are validated. Results thus far indicate that most of the in vitro collection is genetically stable, supporting the use of the tissue culture protocols; however, questions have arisen regarding the optimal duration after which accessions should be genetically verified; how the work can be better organized and communicated, and the implications of the type of somaclonal variations observed. A large body of information has been generated and is accessible to the public via MGIS, including documentation (data and photographs) for 540 accessions, covering 63% of the collection currently available for distribution. This information adds significant value to the ITC collection and is a clear asset to the Musa community. |
| format | Conference Paper |
| id | CGSpace73057 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | International Society for Horticultural Science |
| publisherStr | International Society for Horticultural Science |
| record_format | dspace |
| spelling | CGSpace730572024-06-26T09:37:34Z The field verification activity: a cooperative approach to the management of the global Musa in vitro collection at the International Transit Centre Chase, R. Sardos, J. Ruas, M. Houwe, Ines van den Roux, N. Hribova, E. Doležel, Jaroslav bananas agronomic characters somaclonal variation information systems germplasm collections The International Transit Centre (ITC) (Bioversity International, KULeuven, Belgium) currently holds the largest ex situ collection of Musa germplasm, partly available to researchers, breeders and the larger Musa community via the online ordering system in the Musa Germplasm Information System (MGIS). It is thus extremely important that somaclonal variants and mislabeled accessions are removed from the collection to ensure that users have access to a valid and reliable source of Musa genetic resources. Through Bioversity's Field Verification (FV) activity, a combination of morphological and molecular characterization along with a validation by a panel of experts is used to assess the genetic integrity of the Musa accessions held at ITC. The FV includes ploidy determination and SSR and DArT fingerprinting data (produced by the Musa Genotyping Centre, Institute of Experimental Botany, Czech Republic and DArT P/L, Australia) combined with minimum descriptors and standard photographs of accessions in partners' field collections. The compiled molecular and morphological data are sent to a panel of ten Musa taxonomists in charge of the validation of the accessions' genetic integrity. This workflow allows the quality control of the germplasm available to users while adding value to the content of the ITC by producing standardized characterization data. Status has been determined for 370 of 832 accessions sent to field partners, and the first round will be completed when all virus-free accessions are validated. Results thus far indicate that most of the in vitro collection is genetically stable, supporting the use of the tissue culture protocols; however, questions have arisen regarding the optimal duration after which accessions should be genetically verified; how the work can be better organized and communicated, and the implications of the type of somaclonal variations observed. A large body of information has been generated and is accessible to the public via MGIS, including documentation (data and photographs) for 540 accessions, covering 63% of the collection currently available for distribution. This information adds significant value to the ITC collection and is a clear asset to the Musa community. 2016-03 2016-04-22T07:53:56Z 2016-04-22T07:53:56Z Conference Paper https://hdl.handle.net/10568/73057 en https://hdl.handle.net/10568/72946 Limited Access International Society for Horticultural Science Chase, R.; Sardos, J.; Ruas, M.; Van den houwe, I.; Roux, N.; Hribova, E.; Dolezel, J. (2016) The field verification activity: a cooperative approach to the management of the global Musa in vitro collection at the International Transit Centre. In: Proceedings. IX International Symposium on Banana: ISHS-ProMusa Symposium on Unravelling the Banana's Genomic Potential. (Smith, M. et al (eds.)) Acta Horticulturae, 1114: p. 61-66. Leuven (Belgium), ISHS. ISBN: 978-94-62611-08-5 |
| spellingShingle | bananas agronomic characters somaclonal variation information systems germplasm collections Chase, R. Sardos, J. Ruas, M. Houwe, Ines van den Roux, N. Hribova, E. Doležel, Jaroslav The field verification activity: a cooperative approach to the management of the global Musa in vitro collection at the International Transit Centre |
| title | The field verification activity: a cooperative approach to the management of the global Musa in vitro collection at the International Transit Centre |
| title_full | The field verification activity: a cooperative approach to the management of the global Musa in vitro collection at the International Transit Centre |
| title_fullStr | The field verification activity: a cooperative approach to the management of the global Musa in vitro collection at the International Transit Centre |
| title_full_unstemmed | The field verification activity: a cooperative approach to the management of the global Musa in vitro collection at the International Transit Centre |
| title_short | The field verification activity: a cooperative approach to the management of the global Musa in vitro collection at the International Transit Centre |
| title_sort | field verification activity a cooperative approach to the management of the global musa in vitro collection at the international transit centre |
| topic | bananas agronomic characters somaclonal variation information systems germplasm collections |
| url | https://hdl.handle.net/10568/73057 |
| work_keys_str_mv | AT chaser thefieldverificationactivityacooperativeapproachtothemanagementoftheglobalmusainvitrocollectionattheinternationaltransitcentre AT sardosj thefieldverificationactivityacooperativeapproachtothemanagementoftheglobalmusainvitrocollectionattheinternationaltransitcentre AT ruasm thefieldverificationactivityacooperativeapproachtothemanagementoftheglobalmusainvitrocollectionattheinternationaltransitcentre AT houweinesvanden thefieldverificationactivityacooperativeapproachtothemanagementoftheglobalmusainvitrocollectionattheinternationaltransitcentre AT rouxn thefieldverificationactivityacooperativeapproachtothemanagementoftheglobalmusainvitrocollectionattheinternationaltransitcentre AT hribovae thefieldverificationactivityacooperativeapproachtothemanagementoftheglobalmusainvitrocollectionattheinternationaltransitcentre AT dolezeljaroslav thefieldverificationactivityacooperativeapproachtothemanagementoftheglobalmusainvitrocollectionattheinternationaltransitcentre AT chaser fieldverificationactivityacooperativeapproachtothemanagementoftheglobalmusainvitrocollectionattheinternationaltransitcentre AT sardosj fieldverificationactivityacooperativeapproachtothemanagementoftheglobalmusainvitrocollectionattheinternationaltransitcentre AT ruasm fieldverificationactivityacooperativeapproachtothemanagementoftheglobalmusainvitrocollectionattheinternationaltransitcentre AT houweinesvanden fieldverificationactivityacooperativeapproachtothemanagementoftheglobalmusainvitrocollectionattheinternationaltransitcentre AT rouxn fieldverificationactivityacooperativeapproachtothemanagementoftheglobalmusainvitrocollectionattheinternationaltransitcentre AT hribovae fieldverificationactivityacooperativeapproachtothemanagementoftheglobalmusainvitrocollectionattheinternationaltransitcentre AT dolezeljaroslav fieldverificationactivityacooperativeapproachtothemanagementoftheglobalmusainvitrocollectionattheinternationaltransitcentre |