Identification of presumed ancestral DNA sequences of phaseolin in Phaseolus vulgaris
Common bean (Phaseolus vulgaris) consists oftwo major geographic gene pools, one distributed in Mexico, Central America, and Colombia and the other in the southern Andes (southern Peru, Bolivia, and Argentina). Amplification and sequencing of members of the multigene family coding for phaseolin...
| Autores principales: | , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
1995
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| Acceso en línea: | https://hdl.handle.net/10568/73053 |
| _version_ | 1855521526476963840 |
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| author | Kami, James Becerra Velásquez, Viviana L. Debouck, Daniel G. Gepts, Paul L. |
| author_browse | Becerra Velásquez, Viviana L. Debouck, Daniel G. Gepts, Paul L. Kami, James |
| author_facet | Kami, James Becerra Velásquez, Viviana L. Debouck, Daniel G. Gepts, Paul L. |
| author_sort | Kami, James |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Common bean (Phaseolus vulgaris) consists
oftwo major geographic gene pools, one distributed in Mexico,
Central America, and Colombia and the other in the southern
Andes (southern Peru, Bolivia, and Argentina). Amplification
and sequencing of members of the multigene family coding for
phaseolin, the major seed storage protein of the common bean,
provide evidence for accumulation of tandem direct repeats in
both introns and exons during evolution of the multigene
family in this species. The presumed ancestral phaseolin
sequences, without tandem repeats, were found in recently
discovered but nearly extinct wild common bean populations
of Ecuador and northern Peru that are intermediate between
the two major gene pools of the species based on geographical
and molecular arguments. Our results illustrate the usefulness
of tandem direct repeats in establishing the polarity of
DNA sequence divergence and therefore in proposing phylogenies. |
| format | Journal Article |
| id | CGSpace73053 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1995 |
| publishDateRange | 1995 |
| publishDateSort | 1995 |
| record_format | dspace |
| spelling | CGSpace730532024-09-30T11:32:42Z Identification of presumed ancestral DNA sequences of phaseolin in Phaseolus vulgaris Kami, James Becerra Velásquez, Viviana L. Debouck, Daniel G. Gepts, Paul L. Common bean (Phaseolus vulgaris) consists oftwo major geographic gene pools, one distributed in Mexico, Central America, and Colombia and the other in the southern Andes (southern Peru, Bolivia, and Argentina). Amplification and sequencing of members of the multigene family coding for phaseolin, the major seed storage protein of the common bean, provide evidence for accumulation of tandem direct repeats in both introns and exons during evolution of the multigene family in this species. The presumed ancestral phaseolin sequences, without tandem repeats, were found in recently discovered but nearly extinct wild common bean populations of Ecuador and northern Peru that are intermediate between the two major gene pools of the species based on geographical and molecular arguments. Our results illustrate the usefulness of tandem direct repeats in establishing the polarity of DNA sequence divergence and therefore in proposing phylogenies. 1995-02-14 2016-04-21T18:45:51Z 2016-04-21T18:45:51Z Journal Article https://hdl.handle.net/10568/73053 en Open Access Kami, James; Becerra Velásquez, Viviana; Debouck, Daniel G; Gepts, Paul. 1995. Identification of presumed ancestral DNA sequences of phaseolin in Phaseolus vulgaris. Proceedings of the National Academy of Sciences of the United States of America. Vol. 92, pp. 1101-1104, February 1995. |
| spellingShingle | Kami, James Becerra Velásquez, Viviana L. Debouck, Daniel G. Gepts, Paul L. Identification of presumed ancestral DNA sequences of phaseolin in Phaseolus vulgaris |
| title | Identification of presumed ancestral DNA sequences of phaseolin in Phaseolus vulgaris |
| title_full | Identification of presumed ancestral DNA sequences of phaseolin in Phaseolus vulgaris |
| title_fullStr | Identification of presumed ancestral DNA sequences of phaseolin in Phaseolus vulgaris |
| title_full_unstemmed | Identification of presumed ancestral DNA sequences of phaseolin in Phaseolus vulgaris |
| title_short | Identification of presumed ancestral DNA sequences of phaseolin in Phaseolus vulgaris |
| title_sort | identification of presumed ancestral dna sequences of phaseolin in phaseolus vulgaris |
| url | https://hdl.handle.net/10568/73053 |
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