Are you really my clone? Identity verification of the in-trust sweetpotato collection at the International Potato Center.
The global in-trust sweetpotato collection maintained by the International Potato Center (CIP) in Lima, Peru consists of over 5,000 cultivated sweetpotato accessions maintained as clones in vitro as well as over 1,000 accessions from 67 species of Ipomoea maintained as seed populations. The clonal s...
| Autores principales: | , , , , |
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| Formato: | Póster |
| Lenguaje: | Inglés |
| Publicado: |
PAG
2016
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/72910 |
| _version_ | 1855534688955793408 |
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| author | Ellis, David Barkley, N.A. Rossel, G. Khan, A. Kilian, Andrzej |
| author_browse | Barkley, N.A. Ellis, David Khan, A. Kilian, Andrzej Rossel, G. |
| author_facet | Ellis, David Barkley, N.A. Rossel, G. Khan, A. Kilian, Andrzej |
| author_sort | Ellis, David |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The global in-trust sweetpotato collection maintained by the International Potato Center (CIP) in Lima, Peru consists of over 5,000 cultivated sweetpotato accessions maintained as clones in vitro as well as over 1,000 accessions from 67 species of Ipomoea maintained as seed populations. The clonal sweetpotato collection at CIP was initiated in the 1980’s and for 60% of the collection, original material still exists as potted plants in the greenhouse. This provides a unique opportunity where genetic integrity of a clonal collection, maintained in vitro for the past thirty years, can be confirmed by a side-by-side comparison of the same accession from the greenhouse. Initial molecular comparison is done using a set of twenty SSR primers followed by side-by-side comparison in the field using 30 morphological descriptors. Confirmation of identity requires both genetic and morphological analysis as a low percentage of the accessions appear to be duplicates based on SSR yet are morphologically distinct. Historical morphological descriptor data is used as a check to confirm identity and is being used as the sole check for accessions where we do not have original material for comparison. SSR results from 70% of the collection has confirmed that 85% of the in vitro accessions are true-to-type. In vitro accessions which are not true-to-type are reisolated and cleaned of viruses from the confirmed true-to-type greenhouse accessions. Accessions which are true-to-type are fingerprinted using DArTseq to provide a sequence-based fingerprint. |
| format | Poster |
| id | CGSpace72910 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | PAG |
| publisherStr | PAG |
| record_format | dspace |
| spelling | CGSpace729102025-11-06T13:37:36Z Are you really my clone? Identity verification of the in-trust sweetpotato collection at the International Potato Center. Ellis, David Barkley, N.A. Rossel, G. Khan, A. Kilian, Andrzej sweet potatoes quality controls gene banks The global in-trust sweetpotato collection maintained by the International Potato Center (CIP) in Lima, Peru consists of over 5,000 cultivated sweetpotato accessions maintained as clones in vitro as well as over 1,000 accessions from 67 species of Ipomoea maintained as seed populations. The clonal sweetpotato collection at CIP was initiated in the 1980’s and for 60% of the collection, original material still exists as potted plants in the greenhouse. This provides a unique opportunity where genetic integrity of a clonal collection, maintained in vitro for the past thirty years, can be confirmed by a side-by-side comparison of the same accession from the greenhouse. Initial molecular comparison is done using a set of twenty SSR primers followed by side-by-side comparison in the field using 30 morphological descriptors. Confirmation of identity requires both genetic and morphological analysis as a low percentage of the accessions appear to be duplicates based on SSR yet are morphologically distinct. Historical morphological descriptor data is used as a check to confirm identity and is being used as the sole check for accessions where we do not have original material for comparison. SSR results from 70% of the collection has confirmed that 85% of the in vitro accessions are true-to-type. In vitro accessions which are not true-to-type are reisolated and cleaned of viruses from the confirmed true-to-type greenhouse accessions. Accessions which are true-to-type are fingerprinted using DArTseq to provide a sequence-based fingerprint. 2016 2016-04-14T19:55:33Z 2016-04-14T19:55:33Z Poster https://hdl.handle.net/10568/72910 en Open Access application/pdf PAG Ellis, D.; Barkley, N.; Rossel, G.; Robles, R.; Khan, A.; Kilian, A. 2016. Are you really my clone? Identity verification of the in-trust sweetpotato collection at the International Potato Center. 24. Plant and Animal Genome Conference. San Diego (USA). 09-13 Jan 2015. San Diego (USA). PAG. 1 p. |
| spellingShingle | sweet potatoes quality controls gene banks Ellis, David Barkley, N.A. Rossel, G. Khan, A. Kilian, Andrzej Are you really my clone? Identity verification of the in-trust sweetpotato collection at the International Potato Center. |
| title | Are you really my clone? Identity verification of the in-trust sweetpotato collection at the International Potato Center. |
| title_full | Are you really my clone? Identity verification of the in-trust sweetpotato collection at the International Potato Center. |
| title_fullStr | Are you really my clone? Identity verification of the in-trust sweetpotato collection at the International Potato Center. |
| title_full_unstemmed | Are you really my clone? Identity verification of the in-trust sweetpotato collection at the International Potato Center. |
| title_short | Are you really my clone? Identity verification of the in-trust sweetpotato collection at the International Potato Center. |
| title_sort | are you really my clone identity verification of the in trust sweetpotato collection at the international potato center |
| topic | sweet potatoes quality controls gene banks |
| url | https://hdl.handle.net/10568/72910 |
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