Recombinant Mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumonia
Mycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia (CBPP), a devastating respiratory disease mainly affecting cattle in sub-Saharan Africa. The current vaccines are based on live-attenuated Mmmstrains and present problems with temperature stability,...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2016
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/72741 |
| _version_ | 1855529048754618368 |
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| author | Nkando, I. Pérez Casal, J. Mwirigi, M. Prysliak, T. Townsend, H. Berberov, E. Kuria, J. Mugambi, J. Soi, R. Liljander, Anne M. Jores, Joerg Gerdts, V. Potter, A. Naessens, Jan Wesonga, H.O. |
| author_browse | Berberov, E. Gerdts, V. Jores, Joerg Kuria, J. Liljander, Anne M. Mugambi, J. Mwirigi, M. Naessens, Jan Nkando, I. Potter, A. Prysliak, T. Pérez Casal, J. Soi, R. Townsend, H. Wesonga, H.O. |
| author_facet | Nkando, I. Pérez Casal, J. Mwirigi, M. Prysliak, T. Townsend, H. Berberov, E. Kuria, J. Mugambi, J. Soi, R. Liljander, Anne M. Jores, Joerg Gerdts, V. Potter, A. Naessens, Jan Wesonga, H.O. |
| author_sort | Nkando, I. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Mycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia (CBPP), a devastating respiratory disease mainly affecting cattle in sub-Saharan Africa. The current vaccines are based on live-attenuated Mmmstrains and present problems with temperature stability, duration of immunity and adverse reactions, thus new vaccines are needed to overcome these issues. We used a reverse vaccinology approach to identify 66 Mmm potential vaccine candidates. The selection and grouping of the antigens was based on the presence of specific antibodies in sera from CBPP-positive animals. The antigens were used to immunize male Boran cattle (Bos indicus) followed by a challenge with the Mmm strain Afadé. Two of the groups immunized with five proteins each showed protection after the Mmm challenge (Groups A and C; P < 0.05) and in one group (Group C) Mmm could not be cultured from lung specimens. A third group (Group N) showed a reduced number of animals with lesions and the cultures for Mmm were also negative. While immunization with some of the antigens conferred protection, others may have increased immune-related pathology. This is the first report that Mmm recombinant proteins have been successfully used to formulate a prototype vaccine and these results pave the way for the development of a novel commercial vaccine. |
| format | Journal Article |
| id | CGSpace72741 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace727412024-05-01T08:17:57Z Recombinant Mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumonia Nkando, I. Pérez Casal, J. Mwirigi, M. Prysliak, T. Townsend, H. Berberov, E. Kuria, J. Mugambi, J. Soi, R. Liljander, Anne M. Jores, Joerg Gerdts, V. Potter, A. Naessens, Jan Wesonga, H.O. animal diseases disease control Mycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia (CBPP), a devastating respiratory disease mainly affecting cattle in sub-Saharan Africa. The current vaccines are based on live-attenuated Mmmstrains and present problems with temperature stability, duration of immunity and adverse reactions, thus new vaccines are needed to overcome these issues. We used a reverse vaccinology approach to identify 66 Mmm potential vaccine candidates. The selection and grouping of the antigens was based on the presence of specific antibodies in sera from CBPP-positive animals. The antigens were used to immunize male Boran cattle (Bos indicus) followed by a challenge with the Mmm strain Afadé. Two of the groups immunized with five proteins each showed protection after the Mmm challenge (Groups A and C; P < 0.05) and in one group (Group C) Mmm could not be cultured from lung specimens. A third group (Group N) showed a reduced number of animals with lesions and the cultures for Mmm were also negative. While immunization with some of the antigens conferred protection, others may have increased immune-related pathology. This is the first report that Mmm recombinant proteins have been successfully used to formulate a prototype vaccine and these results pave the way for the development of a novel commercial vaccine. 2016-03 2016-03-28T20:40:13Z 2016-03-28T20:40:13Z Journal Article https://hdl.handle.net/10568/72741 en Limited Access Elsevier Nkando, I., Perez-Casal, J., Mwirigi, M., Prysliak, T., Townsend, H., Berberov, E., Kuria, J., Mugambi, J., Soi, R., Liljander, A., Jores, J., Gerdts, V., Potter, A., Naessens, J. and Wesonga, H. 2016. Recombinant Mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumonia. Veterinary Immunology and Immunopathology 171:103–114. |
| spellingShingle | animal diseases disease control Nkando, I. Pérez Casal, J. Mwirigi, M. Prysliak, T. Townsend, H. Berberov, E. Kuria, J. Mugambi, J. Soi, R. Liljander, Anne M. Jores, Joerg Gerdts, V. Potter, A. Naessens, Jan Wesonga, H.O. Recombinant Mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumonia |
| title | Recombinant Mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumonia |
| title_full | Recombinant Mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumonia |
| title_fullStr | Recombinant Mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumonia |
| title_full_unstemmed | Recombinant Mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumonia |
| title_short | Recombinant Mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumonia |
| title_sort | recombinant mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumonia |
| topic | animal diseases disease control |
| url | https://hdl.handle.net/10568/72741 |
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