Artificial microRNA-derived resistance to Cassava brown streak disease
Artificial miRNAs (amiRNA) were generated targeting conserved sequences within the genomes of the two causal agents of Cassava brown streak disease (CBSD): Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Transient expression studies on ten amiRNAs targeting 21 nt co...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2016
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/72739 |
| _version_ | 1855534073370378240 |
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| author | Wagaba, Henry Patil, Basavaprabhu L. Mukasa, Settumba Alicai, Titus Fauquet, Claude M. Taylor, Nigel J. |
| author_browse | Alicai, Titus Fauquet, Claude M. Mukasa, Settumba Patil, Basavaprabhu L. Taylor, Nigel J. Wagaba, Henry |
| author_facet | Wagaba, Henry Patil, Basavaprabhu L. Mukasa, Settumba Alicai, Titus Fauquet, Claude M. Taylor, Nigel J. |
| author_sort | Wagaba, Henry |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Artificial miRNAs (amiRNA) were generated targeting conserved sequences within the genomes of the two causal agents of Cassava brown streak disease (CBSD): Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Transient expression studies on ten amiRNAs targeting 21 nt conserved sequences of P1(CBSV and UCBSV), P3(CBSV and UCBSV), CI(UCBSV), NIb(CBSV and UCBSV), CP(UCBSV) and the un-translated region (3′-UTR) were tested in Nicotiana benthamiana. Four out of the ten amiRNAs expressed the corresponding amiRNA at high levels. Transgenic N. benthamiana plants were developed for the four amiRNAs targeting the P1 and NIb genes of CBSV and the P1 and CP genes of UCBSV and shown to accumulate miRNA products. Transgenic plants challenged with CBSV and UCBSV isolates showed resistance levels that ranged between ∼20–60% against CBSV and UCBSV and correlated with expression levels of the transgenically derived miRNAs. MicroRNAs targeting P1 and NIb of CBSV showed protection against CBSV and UCBSV, while amiRNAs targeting the P1 and CP of UCBSV showed protection against UCBSV but were less efficient against CBSV. These results indicate a potential application of amiRNAs for engineering resistance to CBSD-causing viruses in cassava. |
| format | Journal Article |
| id | CGSpace72739 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace727392025-03-13T09:44:52Z Artificial microRNA-derived resistance to Cassava brown streak disease Wagaba, Henry Patil, Basavaprabhu L. Mukasa, Settumba Alicai, Titus Fauquet, Claude M. Taylor, Nigel J. cassava brown streak disease cassava brown streak virus manihot esculenta plant viruses rna sequence viruses genomes disease control resistance virus de las plantas secuencia de arn virus genomas cassava control de enfermedades resistencia Artificial miRNAs (amiRNA) were generated targeting conserved sequences within the genomes of the two causal agents of Cassava brown streak disease (CBSD): Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Transient expression studies on ten amiRNAs targeting 21 nt conserved sequences of P1(CBSV and UCBSV), P3(CBSV and UCBSV), CI(UCBSV), NIb(CBSV and UCBSV), CP(UCBSV) and the un-translated region (3′-UTR) were tested in Nicotiana benthamiana. Four out of the ten amiRNAs expressed the corresponding amiRNA at high levels. Transgenic N. benthamiana plants were developed for the four amiRNAs targeting the P1 and NIb genes of CBSV and the P1 and CP genes of UCBSV and shown to accumulate miRNA products. Transgenic plants challenged with CBSV and UCBSV isolates showed resistance levels that ranged between ∼20–60% against CBSV and UCBSV and correlated with expression levels of the transgenically derived miRNAs. MicroRNAs targeting P1 and NIb of CBSV showed protection against CBSV and UCBSV, while amiRNAs targeting the P1 and CP of UCBSV showed protection against UCBSV but were less efficient against CBSV. These results indicate a potential application of amiRNAs for engineering resistance to CBSD-causing viruses in cassava. 2016-05 2016-03-28T20:23:26Z 2016-03-28T20:23:26Z Journal Article https://hdl.handle.net/10568/72739 en Open Access Elsevier Wagaba, Henry; Patil, Basavaprabhu L.; Mukasa, Settumba; Alicai, Titus; Fauquet, Claude M.; Taylor, Nigel J.. 2016. Artificial microRNA-derived resistance to Cassava brown streak disease. Journal of Virological Methods 231: 38-43. |
| spellingShingle | cassava brown streak disease cassava brown streak virus manihot esculenta plant viruses rna sequence viruses genomes disease control resistance virus de las plantas secuencia de arn virus genomas cassava control de enfermedades resistencia Wagaba, Henry Patil, Basavaprabhu L. Mukasa, Settumba Alicai, Titus Fauquet, Claude M. Taylor, Nigel J. Artificial microRNA-derived resistance to Cassava brown streak disease |
| title | Artificial microRNA-derived resistance to Cassava brown streak disease |
| title_full | Artificial microRNA-derived resistance to Cassava brown streak disease |
| title_fullStr | Artificial microRNA-derived resistance to Cassava brown streak disease |
| title_full_unstemmed | Artificial microRNA-derived resistance to Cassava brown streak disease |
| title_short | Artificial microRNA-derived resistance to Cassava brown streak disease |
| title_sort | artificial microrna derived resistance to cassava brown streak disease |
| topic | cassava brown streak disease cassava brown streak virus manihot esculenta plant viruses rna sequence viruses genomes disease control resistance virus de las plantas secuencia de arn virus genomas cassava control de enfermedades resistencia |
| url | https://hdl.handle.net/10568/72739 |
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