Analysis of the cellular immune responses to vaccines
Flow cytometry, enzyme-linked immunospot (ELISpot) and cellular cytotoxicity assays are powerful tools for studying the cellular immune response towards intracellular pathogens and vaccines in livestock species. Lymphocytes from immunized animals can be purified using Ficoll-Paque density gradient c...
| Autores principales: | , , , , , |
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2016
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/72681 |
| _version_ | 1855530998558621696 |
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| author | Svitek, Nicholas Taracha, E.L.N. Saya, R. Awino, Elias Nene, Vishvanath M. Steinaa, Lucilla |
| author_browse | Awino, Elias Nene, Vishvanath M. Saya, R. Steinaa, Lucilla Svitek, Nicholas Taracha, E.L.N. |
| author_facet | Svitek, Nicholas Taracha, E.L.N. Saya, R. Awino, Elias Nene, Vishvanath M. Steinaa, Lucilla |
| author_sort | Svitek, Nicholas |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Flow cytometry, enzyme-linked immunospot (ELISpot) and cellular cytotoxicity assays are powerful tools for studying the cellular immune response towards intracellular pathogens and vaccines in livestock species. Lymphocytes from immunized animals can be purified using Ficoll-Paque density gradient centrifugation and evaluated for their antigen specificity or reactivity towards a vaccine. Here, we describe staining of bovine lymphocytes with peptide (p)-MHC class I tetramers and antibodies specific towards cellular activation markers for evaluation by multiparametric flow cytometry, as well as interferon (IFN)-γ ELISpot and cytotoxicity using chromium (51Cr) release assays. A small component on the use of immunoinformatics for fine-tuning the identification of a minimal CTL epitope is included. |
| format | Book Chapter |
| id | CGSpace72681 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace726812024-01-17T12:58:34Z Analysis of the cellular immune responses to vaccines Svitek, Nicholas Taracha, E.L.N. Saya, R. Awino, Elias Nene, Vishvanath M. Steinaa, Lucilla vaccines Flow cytometry, enzyme-linked immunospot (ELISpot) and cellular cytotoxicity assays are powerful tools for studying the cellular immune response towards intracellular pathogens and vaccines in livestock species. Lymphocytes from immunized animals can be purified using Ficoll-Paque density gradient centrifugation and evaluated for their antigen specificity or reactivity towards a vaccine. Here, we describe staining of bovine lymphocytes with peptide (p)-MHC class I tetramers and antibodies specific towards cellular activation markers for evaluation by multiparametric flow cytometry, as well as interferon (IFN)-γ ELISpot and cytotoxicity using chromium (51Cr) release assays. A small component on the use of immunoinformatics for fine-tuning the identification of a minimal CTL epitope is included. 2016 2016-03-20T07:36:00Z 2016-03-20T07:36:00Z Book Chapter https://hdl.handle.net/10568/72681 en Limited Access Springer Svitek, N., Taracha, E.L., Saya, R., Awino, E., Nene, V. and Steinaa, L. 2016. Analysis of the cellular immune responses to vaccines. IN: Brun, A. (ed), Vaccine technologies for veterinary viral diseases: Methods and protocols, vol. 1349. Dordrecht: Springer. pp. 247-262. |
| spellingShingle | vaccines Svitek, Nicholas Taracha, E.L.N. Saya, R. Awino, Elias Nene, Vishvanath M. Steinaa, Lucilla Analysis of the cellular immune responses to vaccines |
| title | Analysis of the cellular immune responses to vaccines |
| title_full | Analysis of the cellular immune responses to vaccines |
| title_fullStr | Analysis of the cellular immune responses to vaccines |
| title_full_unstemmed | Analysis of the cellular immune responses to vaccines |
| title_short | Analysis of the cellular immune responses to vaccines |
| title_sort | analysis of the cellular immune responses to vaccines |
| topic | vaccines |
| url | https://hdl.handle.net/10568/72681 |
| work_keys_str_mv | AT sviteknicholas analysisofthecellularimmuneresponsestovaccines AT tarachaeln analysisofthecellularimmuneresponsestovaccines AT sayar analysisofthecellularimmuneresponsestovaccines AT awinoelias analysisofthecellularimmuneresponsestovaccines AT nenevishvanathm analysisofthecellularimmuneresponsestovaccines AT steinaalucilla analysisofthecellularimmuneresponsestovaccines |