High occurrence of mitochondrial heteroplasmy in nepalese indigenous sheep (Ovis aries) compared to chinese sheep
Heteroplasmy due to length polymorphism with tandem repeats in mtDNAs within individual was hardly studied in domestic animals. In the present study, we identified intra-individual length variation in the control region of mtDNAs in Nepalese sheep by molecular cloning and sequencing techniques. We o...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Informa UK Limited
2016
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/72466 |
| _version_ | 1855521857742045184 |
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| author | Gorkhali, N.A. Lin Jiang Shrestha, B.S. Xiao-Hong He Qian-Jun Zhao Han Jianlin Yue-Hui Ma |
| author_browse | Gorkhali, N.A. Han Jianlin Lin Jiang Qian-Jun Zhao Shrestha, B.S. Xiao-Hong He Yue-Hui Ma |
| author_facet | Gorkhali, N.A. Lin Jiang Shrestha, B.S. Xiao-Hong He Qian-Jun Zhao Han Jianlin Yue-Hui Ma |
| author_sort | Gorkhali, N.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Heteroplasmy due to length polymorphism with tandem repeats in mtDNAs within individual was hardly studied in domestic animals. In the present study, we identified intra-individual length variation in the control region of mtDNAs in Nepalese sheep by molecular cloning and sequencing techniques. We observed one to four tandem repeats of a 75-bp nucleotide sequences in the mtDNA control region in 45% of the total Nepalese sheep sampled in contrast to the Chinese sheep, indicating that the heteroplasmy is specific to Nepalese sheep. The high rate of heteroplasmy in Nepalese sheep could be a resultant of the mtDNA mutation and independent segregation at intra-individual level or a strand slippage and mispairing during the replication. |
| format | Journal Article |
| id | CGSpace72466 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Informa UK Limited |
| publisherStr | Informa UK Limited |
| record_format | dspace |
| spelling | CGSpace724662024-05-01T08:16:37Z High occurrence of mitochondrial heteroplasmy in nepalese indigenous sheep (Ovis aries) compared to chinese sheep Gorkhali, N.A. Lin Jiang Shrestha, B.S. Xiao-Hong He Qian-Jun Zhao Han Jianlin Yue-Hui Ma sheep indigenous breeds research Heteroplasmy due to length polymorphism with tandem repeats in mtDNAs within individual was hardly studied in domestic animals. In the present study, we identified intra-individual length variation in the control region of mtDNAs in Nepalese sheep by molecular cloning and sequencing techniques. We observed one to four tandem repeats of a 75-bp nucleotide sequences in the mtDNA control region in 45% of the total Nepalese sheep sampled in contrast to the Chinese sheep, indicating that the heteroplasmy is specific to Nepalese sheep. The high rate of heteroplasmy in Nepalese sheep could be a resultant of the mtDNA mutation and independent segregation at intra-individual level or a strand slippage and mispairing during the replication. 2016-07-03 2016-03-06T15:53:49Z 2016-03-06T15:53:49Z Journal Article https://hdl.handle.net/10568/72466 en Limited Access Informa UK Limited Gorkhali, N.A., Lin Jiang, Shrestha, B.S., Xiao-Hong He, Qian Junzhao, Jian-Lin Han and Yue-Hui Ma. 2015. High occurrence of mitochondrial heteroplasmy in nepalese indigenous sheep (Ovis aries) compared to chinese sheep. Mitochondrial DNA 18: 1-3. |
| spellingShingle | sheep indigenous breeds research Gorkhali, N.A. Lin Jiang Shrestha, B.S. Xiao-Hong He Qian-Jun Zhao Han Jianlin Yue-Hui Ma High occurrence of mitochondrial heteroplasmy in nepalese indigenous sheep (Ovis aries) compared to chinese sheep |
| title | High occurrence of mitochondrial heteroplasmy in nepalese indigenous sheep (Ovis aries) compared to chinese sheep |
| title_full | High occurrence of mitochondrial heteroplasmy in nepalese indigenous sheep (Ovis aries) compared to chinese sheep |
| title_fullStr | High occurrence of mitochondrial heteroplasmy in nepalese indigenous sheep (Ovis aries) compared to chinese sheep |
| title_full_unstemmed | High occurrence of mitochondrial heteroplasmy in nepalese indigenous sheep (Ovis aries) compared to chinese sheep |
| title_short | High occurrence of mitochondrial heteroplasmy in nepalese indigenous sheep (Ovis aries) compared to chinese sheep |
| title_sort | high occurrence of mitochondrial heteroplasmy in nepalese indigenous sheep ovis aries compared to chinese sheep |
| topic | sheep indigenous breeds research |
| url | https://hdl.handle.net/10568/72466 |
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