Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance
Mycoplasma mycoides subsp. capri (Mmc) and subsp. mycoides (Mmm) are important ruminant pathogens worldwide causing diseases such as pleuropneumonia, mastitis and septicaemia. They express galactofuranose residues on their surface, but their role in pathogenesis has not yet been determined. The M. m...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
2016
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/72459 |
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| author | Schieck, Elise G. Lartigue, C. Frey, J. Vozza, N. Hegermann, J. Miller, R.A. Valguarnera, E. Muriuki, C. Meens, J. Nene, Vishvanath M. Naessens, Jan Weber, J. Lowary, T.L. Vashee, S. Feldman, M.F. Jores, Joerg |
| author_browse | Feldman, M.F. Frey, J. Hegermann, J. Jores, Joerg Lartigue, C. Lowary, T.L. Meens, J. Miller, R.A. Muriuki, C. Naessens, Jan Nene, Vishvanath M. Schieck, Elise G. Valguarnera, E. Vashee, S. Vozza, N. Weber, J. |
| author_facet | Schieck, Elise G. Lartigue, C. Frey, J. Vozza, N. Hegermann, J. Miller, R.A. Valguarnera, E. Muriuki, C. Meens, J. Nene, Vishvanath M. Naessens, Jan Weber, J. Lowary, T.L. Vashee, S. Feldman, M.F. Jores, Joerg |
| author_sort | Schieck, Elise G. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Mycoplasma mycoides subsp. capri (Mmc) and subsp. mycoides (Mmm) are important ruminant pathogens worldwide causing diseases such as pleuropneumonia, mastitis and septicaemia. They express galactofuranose residues on their surface, but their role in pathogenesis has not yet been determined. The M. mycoides genomes contain up to several copies of the glf gene, which encodes an enzyme catalysing the last step in the synthesis of galactofuranose. We generated a deletion of the glf gene in a strain of Mmc using genome transplantation and tandem repeat endonuclease coupled cleavage (TREC) with yeast as an intermediary host for the genome editing. As expected, the resulting YCp1.1-Δglf strain did not produce the galactofuranose-containing glycans as shown by immunoblots and immuno-electronmicroscopy employing a galactofuranose specific monoclonal antibody. The mutant lacking galactofuranose exhibited a decreased growth rate and a significantly enhanced adhesion to small ruminant cells. The mutant was also ‘leaking’ as revealed by a β-galactosidase-based assay employing a membrane impermeable substrate. These findings indicate that galactofuranose-containing polysaccharides conceal adhesins and are important for membrane integrity. Unexpectedly, the mutant strain showed increased serum resistance. |
| format | Journal Article |
| id | CGSpace72459 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace724592025-12-08T09:54:28Z Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance Schieck, Elise G. Lartigue, C. Frey, J. Vozza, N. Hegermann, J. Miller, R.A. Valguarnera, E. Muriuki, C. Meens, J. Nene, Vishvanath M. Naessens, Jan Weber, J. Lowary, T.L. Vashee, S. Feldman, M.F. Jores, Joerg animal diseases research Mycoplasma mycoides subsp. capri (Mmc) and subsp. mycoides (Mmm) are important ruminant pathogens worldwide causing diseases such as pleuropneumonia, mastitis and septicaemia. They express galactofuranose residues on their surface, but their role in pathogenesis has not yet been determined. The M. mycoides genomes contain up to several copies of the glf gene, which encodes an enzyme catalysing the last step in the synthesis of galactofuranose. We generated a deletion of the glf gene in a strain of Mmc using genome transplantation and tandem repeat endonuclease coupled cleavage (TREC) with yeast as an intermediary host for the genome editing. As expected, the resulting YCp1.1-Δglf strain did not produce the galactofuranose-containing glycans as shown by immunoblots and immuno-electronmicroscopy employing a galactofuranose specific monoclonal antibody. The mutant lacking galactofuranose exhibited a decreased growth rate and a significantly enhanced adhesion to small ruminant cells. The mutant was also ‘leaking’ as revealed by a β-galactosidase-based assay employing a membrane impermeable substrate. These findings indicate that galactofuranose-containing polysaccharides conceal adhesins and are important for membrane integrity. Unexpectedly, the mutant strain showed increased serum resistance. 2016-01 2016-03-06T15:40:18Z 2016-03-06T15:40:18Z Journal Article https://hdl.handle.net/10568/72459 en Open Access Wiley Schieck, E., Lartigue, C., Frey, J., Vozza, N., Hegermann, J., Miller, R.A., Valguarnera, E., Muriuki, C., Meens, J., Nene, V., Naessens, J., Weber, J., Lowary, T.L., Vashee, S., Feldman, M.F. and Jores, J. 2016. Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance. Molecular Microbiology 99(1): 55–70. |
| spellingShingle | animal diseases research Schieck, Elise G. Lartigue, C. Frey, J. Vozza, N. Hegermann, J. Miller, R.A. Valguarnera, E. Muriuki, C. Meens, J. Nene, Vishvanath M. Naessens, Jan Weber, J. Lowary, T.L. Vashee, S. Feldman, M.F. Jores, Joerg Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance |
| title | Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance |
| title_full | Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance |
| title_fullStr | Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance |
| title_full_unstemmed | Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance |
| title_short | Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance |
| title_sort | galactofuranose in mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance |
| topic | animal diseases research |
| url | https://hdl.handle.net/10568/72459 |
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