Cultivo de protoplastos de yuca y Stylosanthes
Methods of isolation and culture of cassava and Stylosanthes protoplasts are described. The application of these methods requires the routine regeneration of plants from isolated protoplasts. In cassava, protoplast isolation from leaves and shoot tips of plants cultured in vitro and from somatic emb...
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| Formato: | Informe técnico |
| Lenguaje: | Español |
| Publicado: |
International Center for Tropical Agriculture
1985
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/71862 |
| _version_ | 1855530304944472064 |
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| author | Szabados, Laszlo |
| author_browse | Szabados, Laszlo |
| author_facet | Szabados, Laszlo |
| author_sort | Szabados, Laszlo |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Methods of isolation and culture of cassava and Stylosanthes protoplasts are described. The application of these methods requires the routine regeneration of plants from isolated protoplasts. In cassava, protoplast isolation from leaves and shoot tips of plants cultured in vitro and from somatic embryos, differentiated from leaf segments, was possible with an enzyme mixture consisting of Onozuka cellulase, hemicellulase, and Pectolyase. Isolated protoplasts were purified by filtration and cultured in liquid medium; they regenerated cell wall after 1-2 days of culture and presented cell division after 3-4 days. Division frequency observed was 10- 20 percent in protoplast cultures from leaf mesophyll and shoot tips. In cultures of somatic embryos, this frequency was less than 5 percent. Within 3-4 wk., dividing protoplasts formed colonies. The plated colonies formed callus in several culture media. Protoplasts from 10 cassava var. have been isolated and cultured. (CIAT) |
| format | Informe técnico |
| id | CGSpace71862 |
| institution | CGIAR Consortium |
| language | Español |
| publishDate | 1985 |
| publishDateRange | 1985 |
| publishDateSort | 1985 |
| publisher | International Center for Tropical Agriculture |
| publisherStr | International Center for Tropical Agriculture |
| record_format | dspace |
| spelling | CGSpace718622025-11-05T18:00:31Z Cultivo de protoplastos de yuca y Stylosanthes Szabados, Laszlo manihot esculenta tissue culture protoplasts starch crops cultivo de tejidos protoplastos Methods of isolation and culture of cassava and Stylosanthes protoplasts are described. The application of these methods requires the routine regeneration of plants from isolated protoplasts. In cassava, protoplast isolation from leaves and shoot tips of plants cultured in vitro and from somatic embryos, differentiated from leaf segments, was possible with an enzyme mixture consisting of Onozuka cellulase, hemicellulase, and Pectolyase. Isolated protoplasts were purified by filtration and cultured in liquid medium; they regenerated cell wall after 1-2 days of culture and presented cell division after 3-4 days. Division frequency observed was 10- 20 percent in protoplast cultures from leaf mesophyll and shoot tips. In cultures of somatic embryos, this frequency was less than 5 percent. Within 3-4 wk., dividing protoplasts formed colonies. The plated colonies formed callus in several culture media. Protoplasts from 10 cassava var. have been isolated and cultured. (CIAT) 1985 2016-02-29T17:13:08Z 2016-02-29T17:13:08Z Report https://hdl.handle.net/10568/71862 es Open Access application/pdf International Center for Tropical Agriculture SZABADOS, L. 1985. Cultivo de protoplastos de yuca y Stylosanthes. Centro Internacional de Agricultura Tropical (CIAT), Cali, CO. 5 p. (Seminarios internos SE-5-85) |
| spellingShingle | manihot esculenta tissue culture protoplasts starch crops cultivo de tejidos protoplastos Szabados, Laszlo Cultivo de protoplastos de yuca y Stylosanthes |
| title | Cultivo de protoplastos de yuca y Stylosanthes |
| title_full | Cultivo de protoplastos de yuca y Stylosanthes |
| title_fullStr | Cultivo de protoplastos de yuca y Stylosanthes |
| title_full_unstemmed | Cultivo de protoplastos de yuca y Stylosanthes |
| title_short | Cultivo de protoplastos de yuca y Stylosanthes |
| title_sort | cultivo de protoplastos de yuca y stylosanthes |
| topic | manihot esculenta tissue culture protoplasts starch crops cultivo de tejidos protoplastos |
| url | https://hdl.handle.net/10568/71862 |
| work_keys_str_mv | AT szabadoslaszlo cultivodeprotoplastosdeyucaystylosanthes |