Utilization of tissue culture techniques for the conservation and international exchange of cassava (Manihot esculenta) germplasm
With graphs, illustrations, photos, and tables, the utilization of tissue culture techniques for the conservation and international exchange of cassava is briefly described. Likewise, the results of the phenotypic stability of cassava cv. M. Mex 20, M. Col 650, M. Col 2197, CM 305-38, and CM 323-375...
| Main Authors: | , , , , , , , , |
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| Format: | Informe técnico |
| Language: | Inglés |
| Published: |
International Center for Tropical Agriculture
1981
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/71732 |
| _version_ | 1855538294206496768 |
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| author | Roca, W. Narvaez, J Rodríguez, J. Reyes, R. Mafla, G. Beltrán, J. Roa E, Julio César Ramírez, H. Rodríguez, A.M. |
| author_browse | Beltrán, J. Mafla, G. Narvaez, J Ramírez, H. Reyes, R. Roa E, Julio César Roca, W. Rodríguez, A.M. Rodríguez, J. |
| author_facet | Roca, W. Narvaez, J Rodríguez, J. Reyes, R. Mafla, G. Beltrán, J. Roa E, Julio César Ramírez, H. Rodríguez, A.M. |
| author_sort | Roca, W. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | With graphs, illustrations, photos, and tables, the utilization of tissue culture techniques for the conservation and international exchange of cassava is briefly described. Likewise, the results of the phenotypic stability of cassava cv. M. Mex 20, M. Col 650, M. Col 2197, CM 305-38, and CM 323-375, stored under min. growth culture conditions for 42, 12, 18, 30, and 30 mo., resp., are given. To evaluate the phenotypic stability, morphological, agronomic, and biochemical parameters were considered. With the exception of variation in the color of root epidermis and cortex, no changes resulted solely from in vitro storage in any of the morphological, agronomic, and biochemical characters evaluated. Finally the use of a modified Murashige and Skoog medium, supplemented with GA, thiourea, and sucrose, to achieve embryo germination and plant development in 10 wild Manihot spp., is reported. (CIAT) |
| format | Informe técnico |
| id | CGSpace71732 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1981 |
| publishDateRange | 1981 |
| publishDateSort | 1981 |
| publisher | International Center for Tropical Agriculture |
| publisherStr | International Center for Tropical Agriculture |
| record_format | dspace |
| spelling | CGSpace717322025-11-05T18:16:11Z Utilization of tissue culture techniques for the conservation and international exchange of cassava (Manihot esculenta) germplasm Roca, W. Narvaez, J Rodríguez, J. Reyes, R. Mafla, G. Beltrán, J. Roa E, Julio César Ramírez, H. Rodríguez, A.M. manihot esculenta agronomic characters analysis apical meristems biochemistry clones culture media enzymes germplasm laboratory equipment manihot physiology plant anatomy plant development plant physiology plant tissues research storage tissue culture With graphs, illustrations, photos, and tables, the utilization of tissue culture techniques for the conservation and international exchange of cassava is briefly described. Likewise, the results of the phenotypic stability of cassava cv. M. Mex 20, M. Col 650, M. Col 2197, CM 305-38, and CM 323-375, stored under min. growth culture conditions for 42, 12, 18, 30, and 30 mo., resp., are given. To evaluate the phenotypic stability, morphological, agronomic, and biochemical parameters were considered. With the exception of variation in the color of root epidermis and cortex, no changes resulted solely from in vitro storage in any of the morphological, agronomic, and biochemical characters evaluated. Finally the use of a modified Murashige and Skoog medium, supplemented with GA, thiourea, and sucrose, to achieve embryo germination and plant development in 10 wild Manihot spp., is reported. (CIAT) 1981 2016-02-29T17:12:28Z 2016-02-29T17:12:28Z Report https://hdl.handle.net/10568/71732 en Open Access application/pdf International Center for Tropical Agriculture ROCA, W.M.; NARVAEZ, J.; RODRIGUEZ, J.; REYES, R.; MAFLA, G.; BELTRAN, J.; ROA, J.; RAMIREZ, H.; RODRIGUEZ, A. 1981. Utilization of tissue culture techniques for the conservation and international exchange of cassava (Manihot esculenta) germplasm. Centro Internacional de Agricultura Tropical (CIAT), Cali, CO. 22 p. |
| spellingShingle | manihot esculenta agronomic characters analysis apical meristems biochemistry clones culture media enzymes germplasm laboratory equipment manihot physiology plant anatomy plant development plant physiology plant tissues research storage tissue culture Roca, W. Narvaez, J Rodríguez, J. Reyes, R. Mafla, G. Beltrán, J. Roa E, Julio César Ramírez, H. Rodríguez, A.M. Utilization of tissue culture techniques for the conservation and international exchange of cassava (Manihot esculenta) germplasm |
| title | Utilization of tissue culture techniques for the conservation and international exchange of cassava (Manihot esculenta) germplasm |
| title_full | Utilization of tissue culture techniques for the conservation and international exchange of cassava (Manihot esculenta) germplasm |
| title_fullStr | Utilization of tissue culture techniques for the conservation and international exchange of cassava (Manihot esculenta) germplasm |
| title_full_unstemmed | Utilization of tissue culture techniques for the conservation and international exchange of cassava (Manihot esculenta) germplasm |
| title_short | Utilization of tissue culture techniques for the conservation and international exchange of cassava (Manihot esculenta) germplasm |
| title_sort | utilization of tissue culture techniques for the conservation and international exchange of cassava manihot esculenta germplasm |
| topic | manihot esculenta agronomic characters analysis apical meristems biochemistry clones culture media enzymes germplasm laboratory equipment manihot physiology plant anatomy plant development plant physiology plant tissues research storage tissue culture |
| url | https://hdl.handle.net/10568/71732 |
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