Multivariable analysis of the association between antimicrobial use and antimicrobial resistance in Escherichia coli isolated from apparently healthy pigs in Japan

The objective of this study was to investigate the association between antimicrobial agent use and antimicrobial resistance in Escherichia coli isolated from healthy pigs using data from 2004 to 2007 in the Japanese Veterinary Antimicrobial Resistance Monitoring System (JVARM). Fecal E. coli isolate...

Descripción completa

Detalles Bibliográficos
Autores principales: Makita, K., Goto, M., Ozawa, M., Kawanishi, M., Koike, R., Asai, T., Tamura, Y.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Mary Ann Liebert Inc 2016
Materias:
Acceso en línea:https://hdl.handle.net/10568/71239
_version_ 1855515124756905984
author Makita, K.
Goto, M.
Ozawa, M.
Kawanishi, M.
Koike, R.
Asai, T.
Tamura, Y.
author_browse Asai, T.
Goto, M.
Kawanishi, M.
Koike, R.
Makita, K.
Ozawa, M.
Tamura, Y.
author_facet Makita, K.
Goto, M.
Ozawa, M.
Kawanishi, M.
Koike, R.
Asai, T.
Tamura, Y.
author_sort Makita, K.
collection Repository of Agricultural Research Outputs (CGSpace)
description The objective of this study was to investigate the association between antimicrobial agent use and antimicrobial resistance in Escherichia coli isolated from healthy pigs using data from 2004 to 2007 in the Japanese Veterinary Antimicrobial Resistance Monitoring System (JVARM). Fecal E. coli isolates from 250 pigs (one isolate each from a pig per farm) were examined for antimicrobial resistance. Information on the use of antimicrobials within preceding 6 months and types of farms recorded in JVARM was collected and statistically analyzed against the resistance patterns. In the univariate analysis, associations between both therapeutic and feed additive use of antimicrobials, and resistance to dihydrostreptomycin, gentamicin, kanamycin, ampicillin, cefazolin, ceftiofur, oxytetracycline, chloramphenicol, trimethoprim, nalidixic acid, enrofloxacin, colistin, and bicozamycin, and husbandry factors were investigated. In multivariable analysis, generalized estimating equations were used to control geographical intraclass correlation. Confounding for structurally unrelated associations was tested using generalized linear models. The results suggested direct and cross selections in the associations between use of aminoglycosides in reproduction farms and resistance to kanamycin, use of tetracyclines in larger farms and resistance to oxytetracycline, use of beta-lactams and resistance to ampicillin, use of phenicols and resistance to chloramphenicol, and use of fluoroquinolones and resistance to nalidixic acid and enrofloxacin. Coselection was suggested in the use of tetracyclines and chloramphenicol resistance. The associations between use of beta-lactams and dihydrostreptomycin resistance, use of macrolides and ampicillin and oxytetracycline resistance, and use of colistin and kanamycin resistance were significant, but were confounded by the simultaneous use of homologous antimicrobials.
format Journal Article
id CGSpace71239
institution CGIAR Consortium
language Inglés
publishDate 2016
publishDateRange 2016
publishDateSort 2016
publisher Mary Ann Liebert Inc
publisherStr Mary Ann Liebert Inc
record_format dspace
spelling CGSpace712392023-10-02T12:05:21Z Multivariable analysis of the association between antimicrobial use and antimicrobial resistance in Escherichia coli isolated from apparently healthy pigs in Japan Makita, K. Goto, M. Ozawa, M. Kawanishi, M. Koike, R. Asai, T. Tamura, Y. disease control pharmacology immunology microbiology The objective of this study was to investigate the association between antimicrobial agent use and antimicrobial resistance in Escherichia coli isolated from healthy pigs using data from 2004 to 2007 in the Japanese Veterinary Antimicrobial Resistance Monitoring System (JVARM). Fecal E. coli isolates from 250 pigs (one isolate each from a pig per farm) were examined for antimicrobial resistance. Information on the use of antimicrobials within preceding 6 months and types of farms recorded in JVARM was collected and statistically analyzed against the resistance patterns. In the univariate analysis, associations between both therapeutic and feed additive use of antimicrobials, and resistance to dihydrostreptomycin, gentamicin, kanamycin, ampicillin, cefazolin, ceftiofur, oxytetracycline, chloramphenicol, trimethoprim, nalidixic acid, enrofloxacin, colistin, and bicozamycin, and husbandry factors were investigated. In multivariable analysis, generalized estimating equations were used to control geographical intraclass correlation. Confounding for structurally unrelated associations was tested using generalized linear models. The results suggested direct and cross selections in the associations between use of aminoglycosides in reproduction farms and resistance to kanamycin, use of tetracyclines in larger farms and resistance to oxytetracycline, use of beta-lactams and resistance to ampicillin, use of phenicols and resistance to chloramphenicol, and use of fluoroquinolones and resistance to nalidixic acid and enrofloxacin. Coselection was suggested in the use of tetracyclines and chloramphenicol resistance. The associations between use of beta-lactams and dihydrostreptomycin resistance, use of macrolides and ampicillin and oxytetracycline resistance, and use of colistin and kanamycin resistance were significant, but were confounded by the simultaneous use of homologous antimicrobials. 2016-01 2016-02-26T15:51:22Z 2016-02-26T15:51:22Z Journal Article https://hdl.handle.net/10568/71239 en Open Access Mary Ann Liebert Inc Makita, K., Goto, M., Ozawa, M., Kawanishi, M., Koike, R., Asai, T. and Tamura, Y. 2016. Multivariable analysis of the association between antimicrobial use and antimicrobial resistance in Escherichia coli isolated from apparently healthy pigs in Japan. Microbial Drug Resistance 22(1): 28-39.
spellingShingle disease control
pharmacology
immunology
microbiology
Makita, K.
Goto, M.
Ozawa, M.
Kawanishi, M.
Koike, R.
Asai, T.
Tamura, Y.
Multivariable analysis of the association between antimicrobial use and antimicrobial resistance in Escherichia coli isolated from apparently healthy pigs in Japan
title Multivariable analysis of the association between antimicrobial use and antimicrobial resistance in Escherichia coli isolated from apparently healthy pigs in Japan
title_full Multivariable analysis of the association between antimicrobial use and antimicrobial resistance in Escherichia coli isolated from apparently healthy pigs in Japan
title_fullStr Multivariable analysis of the association between antimicrobial use and antimicrobial resistance in Escherichia coli isolated from apparently healthy pigs in Japan
title_full_unstemmed Multivariable analysis of the association between antimicrobial use and antimicrobial resistance in Escherichia coli isolated from apparently healthy pigs in Japan
title_short Multivariable analysis of the association between antimicrobial use and antimicrobial resistance in Escherichia coli isolated from apparently healthy pigs in Japan
title_sort multivariable analysis of the association between antimicrobial use and antimicrobial resistance in escherichia coli isolated from apparently healthy pigs in japan
topic disease control
pharmacology
immunology
microbiology
url https://hdl.handle.net/10568/71239
work_keys_str_mv AT makitak multivariableanalysisoftheassociationbetweenantimicrobialuseandantimicrobialresistanceinescherichiacoliisolatedfromapparentlyhealthypigsinjapan
AT gotom multivariableanalysisoftheassociationbetweenantimicrobialuseandantimicrobialresistanceinescherichiacoliisolatedfromapparentlyhealthypigsinjapan
AT ozawam multivariableanalysisoftheassociationbetweenantimicrobialuseandantimicrobialresistanceinescherichiacoliisolatedfromapparentlyhealthypigsinjapan
AT kawanishim multivariableanalysisoftheassociationbetweenantimicrobialuseandantimicrobialresistanceinescherichiacoliisolatedfromapparentlyhealthypigsinjapan
AT koiker multivariableanalysisoftheassociationbetweenantimicrobialuseandantimicrobialresistanceinescherichiacoliisolatedfromapparentlyhealthypigsinjapan
AT asait multivariableanalysisoftheassociationbetweenantimicrobialuseandantimicrobialresistanceinescherichiacoliisolatedfromapparentlyhealthypigsinjapan
AT tamuray multivariableanalysisoftheassociationbetweenantimicrobialuseandantimicrobialresistanceinescherichiacoliisolatedfromapparentlyhealthypigsinjapan