Biofortified indica rice attains iron and zinc nutrition dietary targets in the field
More than two billion people are micronutrient deficient. Polished grains of popular rice varieties have concentration of approximately 2 μg g−1 iron (Fe) and 16 μg g−1 zinc (Zn). The HarvestPlus breeding programs for biofortified rice target 13 μg g−1 Fe and 28 μg g−1 Zn to reach approximately 30%...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2016
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/70994 |
| _version_ | 1855516150441443328 |
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| author | Trijatmiko, Kurniawan R Dueñas, Conrado Tsakirpaloglou, Nikolaos Torrizo, Lina Mae Arines, Felichi Adeva, Cheryl Balindong, Jeanette Oliva, Norman Sapasap, Maria V Borrero Correa, Jaime C. Rey, Jessica Francisco, Perigio Nelson, Andrew Nakanishi, Hiromi Lombi, Enzo Tako, Elad Glahn, Raymond P. Stangoulis, James C.R. Chadha-Mohanty, Prabhjit Johnson, Alexander A.T. Tohme, Joseph M. Barry, Gerard Slamet-Loedin, Inez H. |
| author_browse | Adeva, Cheryl Balindong, Jeanette Barry, Gerard Borrero Correa, Jaime C. Chadha-Mohanty, Prabhjit Dueñas, Conrado Francisco, Perigio Glahn, Raymond P. Johnson, Alexander A.T. Lombi, Enzo Mae Arines, Felichi Nakanishi, Hiromi Nelson, Andrew Oliva, Norman Rey, Jessica Sapasap, Maria V Slamet-Loedin, Inez H. Stangoulis, James C.R. Tako, Elad Tohme, Joseph M. Torrizo, Lina Trijatmiko, Kurniawan R Tsakirpaloglou, Nikolaos |
| author_facet | Trijatmiko, Kurniawan R Dueñas, Conrado Tsakirpaloglou, Nikolaos Torrizo, Lina Mae Arines, Felichi Adeva, Cheryl Balindong, Jeanette Oliva, Norman Sapasap, Maria V Borrero Correa, Jaime C. Rey, Jessica Francisco, Perigio Nelson, Andrew Nakanishi, Hiromi Lombi, Enzo Tako, Elad Glahn, Raymond P. Stangoulis, James C.R. Chadha-Mohanty, Prabhjit Johnson, Alexander A.T. Tohme, Joseph M. Barry, Gerard Slamet-Loedin, Inez H. |
| author_sort | Trijatmiko, Kurniawan R |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | More than two billion people are micronutrient deficient. Polished grains of popular rice varieties have concentration of approximately 2 μg g−1 iron (Fe) and 16 μg g−1 zinc (Zn). The HarvestPlus breeding programs for biofortified rice target 13 μg g−1 Fe and 28 μg g−1 Zn to reach approximately 30% of the estimated average requirement (EAR). Reports on engineering Fe content in rice have shown an increase up to 18 μg g−1 in glasshouse settings; in contrast, under field conditions, 4 μg g−1 was the highest reported concentration. Here, we report on selected transgenic events, field evaluated in two countries, showing 15 μg g−1 Fe and 45.7 μg g−1 Zn in polished grain. Rigorous selection was applied to 1,689 IR64 transgenic events for insert cleanliness and, trait and agronomic performances. Event NASFer-274 containing rice nicotianamine synthase (OsNAS2) and soybean ferritin (SferH-1) genes showed a single locus insertion without a yield penalty or altered grain quality. Endosperm Fe and Zn enrichment was visualized by X-ray fluorescence imaging. The Caco-2 cell assay indicated that Fe is bioavailable. No harmful heavy metals were detected in the grain. The trait remained stable in different genotype backgrounds. |
| format | Journal Article |
| id | CGSpace70994 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace709942025-03-13T09:43:59Z Biofortified indica rice attains iron and zinc nutrition dietary targets in the field Trijatmiko, Kurniawan R Dueñas, Conrado Tsakirpaloglou, Nikolaos Torrizo, Lina Mae Arines, Felichi Adeva, Cheryl Balindong, Jeanette Oliva, Norman Sapasap, Maria V Borrero Correa, Jaime C. Rey, Jessica Francisco, Perigio Nelson, Andrew Nakanishi, Hiromi Lombi, Enzo Tako, Elad Glahn, Raymond P. Stangoulis, James C.R. Chadha-Mohanty, Prabhjit Johnson, Alexander A.T. Tohme, Joseph M. Barry, Gerard Slamet-Loedin, Inez H. oryza sativa rice food enrichment human nutrition nutrient improvement plant breeding yields arroz enriquecimiento de los alimentos nutrición humana mejoramiento nutricional fitomejoramiento rendimiento More than two billion people are micronutrient deficient. Polished grains of popular rice varieties have concentration of approximately 2 μg g−1 iron (Fe) and 16 μg g−1 zinc (Zn). The HarvestPlus breeding programs for biofortified rice target 13 μg g−1 Fe and 28 μg g−1 Zn to reach approximately 30% of the estimated average requirement (EAR). Reports on engineering Fe content in rice have shown an increase up to 18 μg g−1 in glasshouse settings; in contrast, under field conditions, 4 μg g−1 was the highest reported concentration. Here, we report on selected transgenic events, field evaluated in two countries, showing 15 μg g−1 Fe and 45.7 μg g−1 Zn in polished grain. Rigorous selection was applied to 1,689 IR64 transgenic events for insert cleanliness and, trait and agronomic performances. Event NASFer-274 containing rice nicotianamine synthase (OsNAS2) and soybean ferritin (SferH-1) genes showed a single locus insertion without a yield penalty or altered grain quality. Endosperm Fe and Zn enrichment was visualized by X-ray fluorescence imaging. The Caco-2 cell assay indicated that Fe is bioavailable. No harmful heavy metals were detected in the grain. The trait remained stable in different genotype backgrounds. 2016-01-25 2016-02-11T20:08:05Z 2016-02-11T20:08:05Z Journal Article https://hdl.handle.net/10568/70994 en Open Access Springer Trijatmiko, Kurniawan R.; Dueñas, Conrado; Tsakirpaloglou, Nikolaos; Torrizo, Lina; Mae Arines, Felichi; Adeva, Cheryl; Balindong, Jeanette; Oliva, Norman; Sapasap, Maria V.; Borrero, Jaime; Rey, Jessica; Francisco, Perigio; Nelson, Andy; Nakanishi, Hiromi; Lombi, Enzo; Tako, Elad; Glahn, Raymond P.; Stangoulis, James; Chadha-Mohanty, Prabhjit; Johnson, Alexander A. T.; Tohme, Joseph M.; Barry, Gerard; Slamet-Loedin, Inez H.. 2016. Biofortified indica rice attains iron and zinc nutrition dietary targets in the field. Scientific reports 6:19792. |
| spellingShingle | oryza sativa rice food enrichment human nutrition nutrient improvement plant breeding yields arroz enriquecimiento de los alimentos nutrición humana mejoramiento nutricional fitomejoramiento rendimiento Trijatmiko, Kurniawan R Dueñas, Conrado Tsakirpaloglou, Nikolaos Torrizo, Lina Mae Arines, Felichi Adeva, Cheryl Balindong, Jeanette Oliva, Norman Sapasap, Maria V Borrero Correa, Jaime C. Rey, Jessica Francisco, Perigio Nelson, Andrew Nakanishi, Hiromi Lombi, Enzo Tako, Elad Glahn, Raymond P. Stangoulis, James C.R. Chadha-Mohanty, Prabhjit Johnson, Alexander A.T. Tohme, Joseph M. Barry, Gerard Slamet-Loedin, Inez H. Biofortified indica rice attains iron and zinc nutrition dietary targets in the field |
| title | Biofortified indica rice attains iron and zinc nutrition dietary targets in the field |
| title_full | Biofortified indica rice attains iron and zinc nutrition dietary targets in the field |
| title_fullStr | Biofortified indica rice attains iron and zinc nutrition dietary targets in the field |
| title_full_unstemmed | Biofortified indica rice attains iron and zinc nutrition dietary targets in the field |
| title_short | Biofortified indica rice attains iron and zinc nutrition dietary targets in the field |
| title_sort | biofortified indica rice attains iron and zinc nutrition dietary targets in the field |
| topic | oryza sativa rice food enrichment human nutrition nutrient improvement plant breeding yields arroz enriquecimiento de los alimentos nutrición humana mejoramiento nutricional fitomejoramiento rendimiento |
| url | https://hdl.handle.net/10568/70994 |
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