A novel sweet potato potyvirus ORF is expressed via polymerase slippage and suppresses RNA silencing
The single‐stranded, positive‐sense RNA genome of viruses in the genus Potyvirus encodes a large polyprotein that is cleaved to yield 10 mature proteins. The first three cleavage products are P1, HCpro and P3. An additional short open reading frame (ORF), called pipo, overlaps the P3 region of the p...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2016
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/70230 |
| _version_ | 1855516654685913088 |
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| author | Untiveros, M. Olspert, A. Firth, A.E. Artola, K. Kreuze, Jan F. Valkonen, J.P.T. |
| author_browse | Artola, K. Firth, A.E. Kreuze, Jan F. Olspert, A. Untiveros, M. Valkonen, J.P.T. |
| author_facet | Untiveros, M. Olspert, A. Firth, A.E. Artola, K. Kreuze, Jan F. Valkonen, J.P.T. |
| author_sort | Untiveros, M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The single‐stranded, positive‐sense RNA genome of viruses in the genus Potyvirus encodes a large polyprotein that is cleaved to yield 10 mature proteins. The first three cleavage products are P1, HCpro and P3. An additional short open reading frame (ORF), called pipo, overlaps the P3 region of the polyprotein ORF. Four related potyviruses infecting sweet potato (Ipomoea batatas) are predicted to contain a third ORF, called pispo, which overlaps the 3′ third of the P1 region. Recently, pipo has been shown to be expressed via polymerase slippage at a conserved GA6 sequence. Here, we show that pispo is also expressed via polymerase slippage at a GA6 sequence, with higher slippage efficiency (∼5%) than at the pipo site (∼1%). Transient expression of recombinant P1 or the ‘transframe’ product, P1N‐PISPO, in Nicotiana benthamiana suppressed local RNA silencing (RNAi), but only P1N‐PISPO inhibited short‐distance movement of the silencing signal. These results reveal that polymerase slippage in potyviruses is not limited to pipo expression, but can be co‐opted for the evolution and expression of further novel gene products. |
| format | Journal Article |
| id | CGSpace70230 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace702302025-05-28T19:55:28Z A novel sweet potato potyvirus ORF is expressed via polymerase slippage and suppresses RNA silencing Untiveros, M. Olspert, A. Firth, A.E. Artola, K. Kreuze, Jan F. Valkonen, J.P.T. sweet potatoes plant viruses gene expression molecular biology The single‐stranded, positive‐sense RNA genome of viruses in the genus Potyvirus encodes a large polyprotein that is cleaved to yield 10 mature proteins. The first three cleavage products are P1, HCpro and P3. An additional short open reading frame (ORF), called pipo, overlaps the P3 region of the polyprotein ORF. Four related potyviruses infecting sweet potato (Ipomoea batatas) are predicted to contain a third ORF, called pispo, which overlaps the 3′ third of the P1 region. Recently, pipo has been shown to be expressed via polymerase slippage at a conserved GA6 sequence. Here, we show that pispo is also expressed via polymerase slippage at a GA6 sequence, with higher slippage efficiency (∼5%) than at the pipo site (∼1%). Transient expression of recombinant P1 or the ‘transframe’ product, P1N‐PISPO, in Nicotiana benthamiana suppressed local RNA silencing (RNAi), but only P1N‐PISPO inhibited short‐distance movement of the silencing signal. These results reveal that polymerase slippage in potyviruses is not limited to pipo expression, but can be co‐opted for the evolution and expression of further novel gene products. 2016-09 2016-02-01T19:51:32Z 2016-02-01T19:51:32Z Journal Article https://hdl.handle.net/10568/70230 en Open Access Wiley Untiveros, M.; Olspert, A.; Artola, K.; Firth, A.E.; Kreuze, J.F.; Valkonen, J.P.T. 2016. A novel sweet potato potyvirus ORF is expressed via polymerase slippage and suppresses RNA silencing. Molecular Plant Pathology. (UK). ISSN 1464-6722. 17(7):1111-1123. |
| spellingShingle | sweet potatoes plant viruses gene expression molecular biology Untiveros, M. Olspert, A. Firth, A.E. Artola, K. Kreuze, Jan F. Valkonen, J.P.T. A novel sweet potato potyvirus ORF is expressed via polymerase slippage and suppresses RNA silencing |
| title | A novel sweet potato potyvirus ORF is expressed via polymerase slippage and suppresses RNA silencing |
| title_full | A novel sweet potato potyvirus ORF is expressed via polymerase slippage and suppresses RNA silencing |
| title_fullStr | A novel sweet potato potyvirus ORF is expressed via polymerase slippage and suppresses RNA silencing |
| title_full_unstemmed | A novel sweet potato potyvirus ORF is expressed via polymerase slippage and suppresses RNA silencing |
| title_short | A novel sweet potato potyvirus ORF is expressed via polymerase slippage and suppresses RNA silencing |
| title_sort | novel sweet potato potyvirus orf is expressed via polymerase slippage and suppresses rna silencing |
| topic | sweet potatoes plant viruses gene expression molecular biology |
| url | https://hdl.handle.net/10568/70230 |
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