Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation

We investigated the fungal entomopathogens Beauveria bassiana and Metarhizium anisopliae to determine if endophytic colonization could be achieved in cassava. An inoculation method based on drenching the soil around cassava stem cuttings using conidial suspensions resulted in endophytic colonization...

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Autores principales: Greenfield, Melinda, Gómez Jiménez, María I., Ortíz, Viviana, Vega, Fernando E., Kramer, Matthew, Parsa, Soroush
Formato: Journal Article
Lenguaje:Inglés
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://hdl.handle.net/10568/70114
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author Greenfield, Melinda
Gómez Jiménez, María I.
Ortíz, Viviana
Vega, Fernando E.
Kramer, Matthew
Parsa, Soroush
author_browse Greenfield, Melinda
Gómez Jiménez, María I.
Kramer, Matthew
Ortíz, Viviana
Parsa, Soroush
Vega, Fernando E.
author_facet Greenfield, Melinda
Gómez Jiménez, María I.
Ortíz, Viviana
Vega, Fernando E.
Kramer, Matthew
Parsa, Soroush
author_sort Greenfield, Melinda
collection Repository of Agricultural Research Outputs (CGSpace)
description We investigated the fungal entomopathogens Beauveria bassiana and Metarhizium anisopliae to determine if endophytic colonization could be achieved in cassava. An inoculation method based on drenching the soil around cassava stem cuttings using conidial suspensions resulted in endophytic colonization of cassava roots by both entomopathogens, though neither was found in the leaves or stems of the treated cassava plants. Both fungal entomopathogens were detected more often in the proximal end of the root than in the distal end. Colonization levels of B. bassiana were higher when plants were sampled at 7–9 days post-inoculation (84%) compared to 47–49 days post-inoculation (40%). In contrast, the colonization levels of M. anisopliae remained constant from 7–9 days post-inoculation (80%) to 47–49 days postinoculation (80%), which suggests M. anisopliae is better able to persist in the soil, or as an endophyte in cassava roots over time. Differences in colonization success and plant growth were found among the fungal entomopathogen treatments.
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spelling CGSpace701142025-03-13T09:45:08Z Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation Greenfield, Melinda Gómez Jiménez, María I. Ortíz, Viviana Vega, Fernando E. Kramer, Matthew Parsa, Soroush beauveria manihot esculenta cassava endophytes pest control entomogenous fungi inoculation metarhizium endofitas control de plagas hongos entomopatógenos inoculación We investigated the fungal entomopathogens Beauveria bassiana and Metarhizium anisopliae to determine if endophytic colonization could be achieved in cassava. An inoculation method based on drenching the soil around cassava stem cuttings using conidial suspensions resulted in endophytic colonization of cassava roots by both entomopathogens, though neither was found in the leaves or stems of the treated cassava plants. Both fungal entomopathogens were detected more often in the proximal end of the root than in the distal end. Colonization levels of B. bassiana were higher when plants were sampled at 7–9 days post-inoculation (84%) compared to 47–49 days post-inoculation (40%). In contrast, the colonization levels of M. anisopliae remained constant from 7–9 days post-inoculation (80%) to 47–49 days postinoculation (80%), which suggests M. anisopliae is better able to persist in the soil, or as an endophyte in cassava roots over time. Differences in colonization success and plant growth were found among the fungal entomopathogen treatments. 2016-04 2016-01-22T16:44:06Z 2016-01-22T16:44:06Z Journal Article https://hdl.handle.net/10568/70114 en Open Access Elsevier Greenfield, Melinda; Gómez-Jiménez, María I.; Ortiz, Viviana; Vega, Fernando E.; Kramer, Matthew; Parsa, Soroush. 2016. Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation. Biological Control . Elsevier B.V., 95: 40-48.
spellingShingle beauveria
manihot esculenta
cassava
endophytes
pest control
entomogenous fungi
inoculation
metarhizium
endofitas
control de plagas
hongos entomopatógenos
inoculación
Greenfield, Melinda
Gómez Jiménez, María I.
Ortíz, Viviana
Vega, Fernando E.
Kramer, Matthew
Parsa, Soroush
Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation
title Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation
title_full Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation
title_fullStr Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation
title_full_unstemmed Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation
title_short Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation
title_sort beauveria bassiana and metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation
topic beauveria
manihot esculenta
cassava
endophytes
pest control
entomogenous fungi
inoculation
metarhizium
endofitas
control de plagas
hongos entomopatógenos
inoculación
url https://hdl.handle.net/10568/70114
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