Removal of leafroll viruses from infected grapevine plants by droplet vitrification
A robust method for removing all microorganisms from infected tissue is important for cultivar imports, germplasm maintenance and to produce healthy grafted material for the grapevine industry. In the droplet vitrification method of cryopreservation, plant tissue pre-treated with a vitrification sol...
| Autores principales: | , , , , , |
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| Formato: | Conference Paper |
| Lenguaje: | Inglés |
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International Society for Horticultural Science
2015
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| Acceso en línea: | https://hdl.handle.net/10568/69421 |
| _version_ | 1855524955408564224 |
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| author | Pathirana, R. McLachlan, A. Hedderley, D. Carra, A. Carimi, F. Panis, Bartholomeus |
| author_browse | Carimi, F. Carra, A. Hedderley, D. McLachlan, A. Panis, Bartholomeus Pathirana, R. |
| author_facet | Pathirana, R. McLachlan, A. Hedderley, D. Carra, A. Carimi, F. Panis, Bartholomeus |
| author_sort | Pathirana, R. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A robust method for removing all microorganisms from infected tissue is important for cultivar imports, germplasm maintenance and to produce healthy grafted material for the grapevine industry. In the droplet vitrification method of cryopreservation, plant tissue pre-treated with a vitrification solution is placed on aluminium foil in a droplet of vitrification solution and directly immersed in liquid nitrogen. Only highly cytoplasmic, non-vacuolar meristematic cells survive freezing. Therefore, cryopreservation can be considered a precise method of meristem culture and is developing into a new method for virus eradication in horticultural species called cryotherapy. To test the suitability of cryotherapy for virus eradication, we used ‘Chardonnay’ and ‘Lakemont Seedless’ infected with Grapevine leafroll associated virus-3 (GLRaV-3, an Ampelovirus), ‘Pinot gris’ and ‘Sauvignon blanc 316’ infected with Grapevine leafroll associated virus-2 (a Closterovirus), and another clone of ‘Sauvignon blanc’ infected with both Grapevine leafroll associated virus-1 (an Ampelovirus) and GLRaV-3. Plants regenerated after cryo-treatment tested negative (DAS-ELISA) for all three viruses, whereas untreated control plants tested positive. Droplet vitrification has the potential to be a novel and precise tool for virus eradication and establishment of high-health grapevine germplasm collections. In addition to removing virus infections, the method provides a cost-effective way of maintaining clonal plant material. |
| format | Conference Paper |
| id | CGSpace69421 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | International Society for Horticultural Science |
| publisherStr | International Society for Horticultural Science |
| record_format | dspace |
| spelling | CGSpace694212025-11-05T07:14:51Z Removal of leafroll viruses from infected grapevine plants by droplet vitrification Pathirana, R. McLachlan, A. Hedderley, D. Carra, A. Carimi, F. Panis, Bartholomeus closteroviridae freezing biological preservation cryopreservation salicylic acids germplasm sucrose vitis vinfera A robust method for removing all microorganisms from infected tissue is important for cultivar imports, germplasm maintenance and to produce healthy grafted material for the grapevine industry. In the droplet vitrification method of cryopreservation, plant tissue pre-treated with a vitrification solution is placed on aluminium foil in a droplet of vitrification solution and directly immersed in liquid nitrogen. Only highly cytoplasmic, non-vacuolar meristematic cells survive freezing. Therefore, cryopreservation can be considered a precise method of meristem culture and is developing into a new method for virus eradication in horticultural species called cryotherapy. To test the suitability of cryotherapy for virus eradication, we used ‘Chardonnay’ and ‘Lakemont Seedless’ infected with Grapevine leafroll associated virus-3 (GLRaV-3, an Ampelovirus), ‘Pinot gris’ and ‘Sauvignon blanc 316’ infected with Grapevine leafroll associated virus-2 (a Closterovirus), and another clone of ‘Sauvignon blanc’ infected with both Grapevine leafroll associated virus-1 (an Ampelovirus) and GLRaV-3. Plants regenerated after cryo-treatment tested negative (DAS-ELISA) for all three viruses, whereas untreated control plants tested positive. Droplet vitrification has the potential to be a novel and precise tool for virus eradication and establishment of high-health grapevine germplasm collections. In addition to removing virus infections, the method provides a cost-effective way of maintaining clonal plant material. 2015-05 2015-12-23T10:46:09Z 2015-12-23T10:46:09Z Conference Paper https://hdl.handle.net/10568/69421 en Limited Access application/pdf International Society for Horticultural Science Pathirana, R.; McLachlan, A.; Hedderley, D.; Carra, A.; Carimi, F.; Panis, B. (2015) Removal of leafroll viruses from infected grapevine plants by droplet vitrification. In VIII International Symposium on In Vitro Culture and Horticultural Breeding. (Canhoto, J.M. et al. (eds.)) Acta Horticulturae 1083 p.491-498 ISSN: 0567-7572 |
| spellingShingle | closteroviridae freezing biological preservation cryopreservation salicylic acids germplasm sucrose vitis vinfera Pathirana, R. McLachlan, A. Hedderley, D. Carra, A. Carimi, F. Panis, Bartholomeus Removal of leafroll viruses from infected grapevine plants by droplet vitrification |
| title | Removal of leafroll viruses from infected grapevine plants by droplet vitrification |
| title_full | Removal of leafroll viruses from infected grapevine plants by droplet vitrification |
| title_fullStr | Removal of leafroll viruses from infected grapevine plants by droplet vitrification |
| title_full_unstemmed | Removal of leafroll viruses from infected grapevine plants by droplet vitrification |
| title_short | Removal of leafroll viruses from infected grapevine plants by droplet vitrification |
| title_sort | removal of leafroll viruses from infected grapevine plants by droplet vitrification |
| topic | closteroviridae freezing biological preservation cryopreservation salicylic acids germplasm sucrose vitis vinfera |
| url | https://hdl.handle.net/10568/69421 |
| work_keys_str_mv | AT pathiranar removalofleafrollvirusesfrominfectedgrapevineplantsbydropletvitrification AT mclachlana removalofleafrollvirusesfrominfectedgrapevineplantsbydropletvitrification AT hedderleyd removalofleafrollvirusesfrominfectedgrapevineplantsbydropletvitrification AT carraa removalofleafrollvirusesfrominfectedgrapevineplantsbydropletvitrification AT carimif removalofleafrollvirusesfrominfectedgrapevineplantsbydropletvitrification AT panisbartholomeus removalofleafrollvirusesfrominfectedgrapevineplantsbydropletvitrification |