Trypanosoma infection rates in Glossina species in Mtito Andei Division, Makueni County, Kenya
African Animal Trypanosomiasis (AAT) transmitted cyclically by tsetse fly (Glossina spp.) is a major obstacle to livestock production in the tropical parts of Africa. The objective of this study was to determine the infection rates of trypanosomes in Glossina species in Mtito Andei Division, Makueni...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Hindawi Limited
2015
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/68938 |
| _version_ | 1855514987390304256 |
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| author | Nthiwa, D.M. Odongo, David O. Ochanda, H. Khamadi, S. Gichimu, B.M. |
| author_browse | Gichimu, B.M. Khamadi, S. Nthiwa, D.M. Ochanda, H. Odongo, David O. |
| author_facet | Nthiwa, D.M. Odongo, David O. Ochanda, H. Khamadi, S. Gichimu, B.M. |
| author_sort | Nthiwa, D.M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | African Animal Trypanosomiasis (AAT) transmitted cyclically by tsetse fly (Glossina spp.) is a major obstacle to livestock production in the tropical parts of Africa. The objective of this study was to determine the infection rates of trypanosomes in Glossina species in Mtito Andei Division, Makueni County, Kenya. Tsetse fly species, G. longipennis and G. pallidipes, were trapped and DNA was isolated from their dissected internal organs (proboscis, salivary glands, and midguts). The DNA was then subjected to a nested PCR assay using internal transcribed spacer primers and individual trypanosome species were identified following agarose gel electrophoresis. Out of the 117 flies trapped in the area 39 (33.3%) were teneral while 78 (67%) were nonteneral. G. pallidipes constituted the largest percentage of 58% while G. longipennis were 42%. The overall trypanosomes infection rate in all nonteneral Glossina spp. was 11.53% with G. longipennis recording the highest infection rate of 23.08% while G. pallidipes had an infection rate of 5.77%. T. vivax was the most infectious (10.26%) compared to T. congolense (1.28%). Mean apparent densities were strongly positively correlated with infection rates (r = 0.95) confirming the importance of this parameter as an indicator of AAT transmission risk. |
| format | Journal Article |
| id | CGSpace68938 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Hindawi Limited |
| publisherStr | Hindawi Limited |
| record_format | dspace |
| spelling | CGSpace689382024-08-27T10:34:50Z Trypanosoma infection rates in Glossina species in Mtito Andei Division, Makueni County, Kenya Nthiwa, D.M. Odongo, David O. Ochanda, H. Khamadi, S. Gichimu, B.M. animal diseases infectious diseases parasitology African Animal Trypanosomiasis (AAT) transmitted cyclically by tsetse fly (Glossina spp.) is a major obstacle to livestock production in the tropical parts of Africa. The objective of this study was to determine the infection rates of trypanosomes in Glossina species in Mtito Andei Division, Makueni County, Kenya. Tsetse fly species, G. longipennis and G. pallidipes, were trapped and DNA was isolated from their dissected internal organs (proboscis, salivary glands, and midguts). The DNA was then subjected to a nested PCR assay using internal transcribed spacer primers and individual trypanosome species were identified following agarose gel electrophoresis. Out of the 117 flies trapped in the area 39 (33.3%) were teneral while 78 (67%) were nonteneral. G. pallidipes constituted the largest percentage of 58% while G. longipennis were 42%. The overall trypanosomes infection rate in all nonteneral Glossina spp. was 11.53% with G. longipennis recording the highest infection rate of 23.08% while G. pallidipes had an infection rate of 5.77%. T. vivax was the most infectious (10.26%) compared to T. congolense (1.28%). Mean apparent densities were strongly positively correlated with infection rates (r = 0.95) confirming the importance of this parameter as an indicator of AAT transmission risk. 2015 2015-11-17T09:35:53Z 2015-11-17T09:35:53Z Journal Article https://hdl.handle.net/10568/68938 en Open Access Hindawi Limited Nthiwa, D.M., Odongo, D.O., Ochanda, H., Khamadi, S. and Gichimu, B.M. 2015. Trypanosoma infection rates in Glossina species in Mtito Andei Division, Makueni County, Kenya. Journal of Parasitology Research 2015(2015): Article ID 607432. |
| spellingShingle | animal diseases infectious diseases parasitology Nthiwa, D.M. Odongo, David O. Ochanda, H. Khamadi, S. Gichimu, B.M. Trypanosoma infection rates in Glossina species in Mtito Andei Division, Makueni County, Kenya |
| title | Trypanosoma infection rates in Glossina species in Mtito Andei Division, Makueni County, Kenya |
| title_full | Trypanosoma infection rates in Glossina species in Mtito Andei Division, Makueni County, Kenya |
| title_fullStr | Trypanosoma infection rates in Glossina species in Mtito Andei Division, Makueni County, Kenya |
| title_full_unstemmed | Trypanosoma infection rates in Glossina species in Mtito Andei Division, Makueni County, Kenya |
| title_short | Trypanosoma infection rates in Glossina species in Mtito Andei Division, Makueni County, Kenya |
| title_sort | trypanosoma infection rates in glossina species in mtito andei division makueni county kenya |
| topic | animal diseases infectious diseases parasitology |
| url | https://hdl.handle.net/10568/68938 |
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