Evidence for negative selection of gene variants that increase dependence on dietary choline in a Gambian cohort

Choline is an essential nutrient, and the amount needed in the diet is modulated by several factors. Given geographical differences in dietary choline intake and disparate frequencies of single-nucleotide polymorphisms (SNPs) in choline metabolism genes between ethnic groups, we tested the hypothesi...

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Main Authors: Silver, M.J., Corbin, K.D., Hellenthal, G., Costa, K.A. da, Domínguez Salas, Paula, Moore, S.E., Owen, J., Prentice, A.M., Hennig, B.J., Zeisel, S.H.
Format: Journal Article
Language:Inglés
Published: Wiley 2015
Online Access:https://hdl.handle.net/10568/68515
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author Silver, M.J.
Corbin, K.D.
Hellenthal, G.
Costa, K.A. da
Domínguez Salas, Paula
Moore, S.E.
Owen, J.
Prentice, A.M.
Hennig, B.J.
Zeisel, S.H.
author_browse Corbin, K.D.
Costa, K.A. da
Domínguez Salas, Paula
Hellenthal, G.
Hennig, B.J.
Moore, S.E.
Owen, J.
Prentice, A.M.
Silver, M.J.
Zeisel, S.H.
author_facet Silver, M.J.
Corbin, K.D.
Hellenthal, G.
Costa, K.A. da
Domínguez Salas, Paula
Moore, S.E.
Owen, J.
Prentice, A.M.
Hennig, B.J.
Zeisel, S.H.
author_sort Silver, M.J.
collection Repository of Agricultural Research Outputs (CGSpace)
description Choline is an essential nutrient, and the amount needed in the diet is modulated by several factors. Given geographical differences in dietary choline intake and disparate frequencies of single-nucleotide polymorphisms (SNPs) in choline metabolism genes between ethnic groups, we tested the hypothesis that 3 SNPs that increase dependence on dietary choline would be under negative selection pressure in settings where choline intake is low: choline dehydrogenase (CHDH) rs12676, methylenetetrahydrofolate reductase 1 (MTHFD1) rs2236225, and phosphatidylethanolamine-N-methyltransferase (PEMT) rs12325817. Evidence of negative selection was assessed in 2 populations: one in The Gambia, West Africa, where there is historic evidence of a choline-poor diet, and the other in the United States, with a comparatively choline-rich diet. We used 2 independent methods, and confirmation of our hypothesis was sought via a comparison with SNP data from the Maasai, an East African population with a genetic background similar to that of Gambians but with a traditional diet that is higher in choline. Our results show that frequencies of SNPs known to increase dependence on dietary choline are significantly reduced in the low-choline setting of The Gambia. Our findings suggest that adequate intake levels of choline may have to be reevaluated in different ethnic groups and highlight a possible approach for identifying novel functional SNPs under the influence of dietary selective pressure.
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spelling CGSpace685152023-12-08T19:25:22Z Evidence for negative selection of gene variants that increase dependence on dietary choline in a Gambian cohort Silver, M.J. Corbin, K.D. Hellenthal, G. Costa, K.A. da Domínguez Salas, Paula Moore, S.E. Owen, J. Prentice, A.M. Hennig, B.J. Zeisel, S.H. Choline is an essential nutrient, and the amount needed in the diet is modulated by several factors. Given geographical differences in dietary choline intake and disparate frequencies of single-nucleotide polymorphisms (SNPs) in choline metabolism genes between ethnic groups, we tested the hypothesis that 3 SNPs that increase dependence on dietary choline would be under negative selection pressure in settings where choline intake is low: choline dehydrogenase (CHDH) rs12676, methylenetetrahydrofolate reductase 1 (MTHFD1) rs2236225, and phosphatidylethanolamine-N-methyltransferase (PEMT) rs12325817. Evidence of negative selection was assessed in 2 populations: one in The Gambia, West Africa, where there is historic evidence of a choline-poor diet, and the other in the United States, with a comparatively choline-rich diet. We used 2 independent methods, and confirmation of our hypothesis was sought via a comparison with SNP data from the Maasai, an East African population with a genetic background similar to that of Gambians but with a traditional diet that is higher in choline. Our results show that frequencies of SNPs known to increase dependence on dietary choline are significantly reduced in the low-choline setting of The Gambia. Our findings suggest that adequate intake levels of choline may have to be reevaluated in different ethnic groups and highlight a possible approach for identifying novel functional SNPs under the influence of dietary selective pressure. 2015-08 2015-10-13T12:33:05Z 2015-10-13T12:33:05Z Journal Article https://hdl.handle.net/10568/68515 en Open Access Wiley Silver, M.J., Corbin, K.D., Hellenthal, G., da Costa, K.-A., Dominguez-Salas, P., Moore, S.E., Owen, J., Prentice, A.M., Hennig, B.J. and Zeisel, S.H. 2015. Evidence for negative selection of gene variants that increase dependence on dietary choline in a Gambian cohort. The FASEB Journal 29(8): 3426-3435.
spellingShingle Silver, M.J.
Corbin, K.D.
Hellenthal, G.
Costa, K.A. da
Domínguez Salas, Paula
Moore, S.E.
Owen, J.
Prentice, A.M.
Hennig, B.J.
Zeisel, S.H.
Evidence for negative selection of gene variants that increase dependence on dietary choline in a Gambian cohort
title Evidence for negative selection of gene variants that increase dependence on dietary choline in a Gambian cohort
title_full Evidence for negative selection of gene variants that increase dependence on dietary choline in a Gambian cohort
title_fullStr Evidence for negative selection of gene variants that increase dependence on dietary choline in a Gambian cohort
title_full_unstemmed Evidence for negative selection of gene variants that increase dependence on dietary choline in a Gambian cohort
title_short Evidence for negative selection of gene variants that increase dependence on dietary choline in a Gambian cohort
title_sort evidence for negative selection of gene variants that increase dependence on dietary choline in a gambian cohort
url https://hdl.handle.net/10568/68515
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