Genomic organization and expression profile of the mucin-associated surface protein (masp) family of the human pathogen Trypanosoma cruzi
A novel large multigene family was recently identified in the human pathogen Trypanosoma cruzi, causative agent of Chagas disease, and corresponds to ?6% of the parasite diploid genome. The predicted gene products, mucin-associated surface proteins (MASPs), are characterized by highly conserved N- a...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Oxford University Press
2009
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/68348 |
| _version_ | 1855526767366766592 |
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| author | Bartholomeu, D.C. Cerqueira, G.C. Leao, A.C.A. DaRocha, W.D. Pais, F.S. Macedo, C. Djikeng, Appolinaire Teixeira, S.M.R. El-Sayed, N.M. |
| author_browse | Bartholomeu, D.C. Cerqueira, G.C. DaRocha, W.D. Djikeng, Appolinaire El-Sayed, N.M. Leao, A.C.A. Macedo, C. Pais, F.S. Teixeira, S.M.R. |
| author_facet | Bartholomeu, D.C. Cerqueira, G.C. Leao, A.C.A. DaRocha, W.D. Pais, F.S. Macedo, C. Djikeng, Appolinaire Teixeira, S.M.R. El-Sayed, N.M. |
| author_sort | Bartholomeu, D.C. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A novel large multigene family was recently identified in the human pathogen Trypanosoma cruzi, causative agent of Chagas disease, and corresponds to ?6% of the parasite diploid genome. The predicted gene products, mucin-associated surface proteins (MASPs), are characterized by highly conserved N- and C-terminal domains and a strikingly variable and repetitive central region. We report here an analysis of the genomic organization and expression profile of masp genes. Masps are not randomly distributed throughout the genome but instead are clustered with genes encoding mucin and other surface protein families. Masp transcripts vary in size, are preferentially expressed during the trypomastigote stage and contain highly conserved 5? and 3? untranslated regions. A sequence analysis of a trypomastigote cDNA library reveals the expression of multiple masp variants with a bias towards a particular masp subgroup. Immunofluorescence assays using antibodies generated against a MASP peptide reveals that the expression of particular MASPs at the cell membrane is limited to subsets of the parasite population. Western blots of phosphatidylinositol-specific phospholipase C (PI-PLC)-treated parasites suggest that MASP may be GPI-anchored and shed into the medium culture, thus contributing to the large repertoire of parasite polypeptides that are exposed to the host immune system. |
| format | Journal Article |
| id | CGSpace68348 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace683482025-12-08T09:54:28Z Genomic organization and expression profile of the mucin-associated surface protein (masp) family of the human pathogen Trypanosoma cruzi Bartholomeu, D.C. Cerqueira, G.C. Leao, A.C.A. DaRocha, W.D. Pais, F.S. Macedo, C. Djikeng, Appolinaire Teixeira, S.M.R. El-Sayed, N.M. trypanosoma health A novel large multigene family was recently identified in the human pathogen Trypanosoma cruzi, causative agent of Chagas disease, and corresponds to ?6% of the parasite diploid genome. The predicted gene products, mucin-associated surface proteins (MASPs), are characterized by highly conserved N- and C-terminal domains and a strikingly variable and repetitive central region. We report here an analysis of the genomic organization and expression profile of masp genes. Masps are not randomly distributed throughout the genome but instead are clustered with genes encoding mucin and other surface protein families. Masp transcripts vary in size, are preferentially expressed during the trypomastigote stage and contain highly conserved 5? and 3? untranslated regions. A sequence analysis of a trypomastigote cDNA library reveals the expression of multiple masp variants with a bias towards a particular masp subgroup. Immunofluorescence assays using antibodies generated against a MASP peptide reveals that the expression of particular MASPs at the cell membrane is limited to subsets of the parasite population. Western blots of phosphatidylinositol-specific phospholipase C (PI-PLC)-treated parasites suggest that MASP may be GPI-anchored and shed into the medium culture, thus contributing to the large repertoire of parasite polypeptides that are exposed to the host immune system. 2009-06 2015-09-30T10:55:51Z 2015-09-30T10:55:51Z Journal Article https://hdl.handle.net/10568/68348 en Open Access Oxford University Press Bartholomeu, D.C., Cerqueira, G.C., Leão, A.C.A., daRocha, W.D., Pais, F.S., Macedo, C., Djikeng, A., Teixeira, S.M.R. and El-Sayed, N.M. 2009. Genomic organization and expression profile of the mucin-associated surface protein (masp) family of the human pathogen Trypanosoma cruzi. Nucleic Acids Research 37(10): 3407-3417. |
| spellingShingle | trypanosoma health Bartholomeu, D.C. Cerqueira, G.C. Leao, A.C.A. DaRocha, W.D. Pais, F.S. Macedo, C. Djikeng, Appolinaire Teixeira, S.M.R. El-Sayed, N.M. Genomic organization and expression profile of the mucin-associated surface protein (masp) family of the human pathogen Trypanosoma cruzi |
| title | Genomic organization and expression profile of the mucin-associated surface protein (masp) family of the human pathogen Trypanosoma cruzi |
| title_full | Genomic organization and expression profile of the mucin-associated surface protein (masp) family of the human pathogen Trypanosoma cruzi |
| title_fullStr | Genomic organization and expression profile of the mucin-associated surface protein (masp) family of the human pathogen Trypanosoma cruzi |
| title_full_unstemmed | Genomic organization and expression profile of the mucin-associated surface protein (masp) family of the human pathogen Trypanosoma cruzi |
| title_short | Genomic organization and expression profile of the mucin-associated surface protein (masp) family of the human pathogen Trypanosoma cruzi |
| title_sort | genomic organization and expression profile of the mucin associated surface protein masp family of the human pathogen trypanosoma cruzi |
| topic | trypanosoma health |
| url | https://hdl.handle.net/10568/68348 |
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