Towards understanding the diversity of banana bunchy top virus in the Great Lakes region of Africa

The genetic variability of banana bunchy top virus (BBTV) isolates from the Great Lakes region of Africa (GLRA) spanning Burundi, the Democratic Republic of the Congo and Rwanda was assessed to better understand BBTV diversity and its epidemiology for improved disease management. DNA-R and DNA-S fra...

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Main Authors: Niyongere, C., Lepoint, P.C.E., Losenge, T., Blomme, Guy, Ateka, E.M.
Format: Journal Article
Language:Inglés
Published: Academic Journals 2015
Subjects:
Online Access:https://hdl.handle.net/10568/68142
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author Niyongere, C.
Lepoint, P.C.E.
Losenge, T.
Blomme, Guy
Ateka, E.M.
author_browse Ateka, E.M.
Blomme, Guy
Lepoint, P.C.E.
Losenge, T.
Niyongere, C.
author_facet Niyongere, C.
Lepoint, P.C.E.
Losenge, T.
Blomme, Guy
Ateka, E.M.
author_sort Niyongere, C.
collection Repository of Agricultural Research Outputs (CGSpace)
description The genetic variability of banana bunchy top virus (BBTV) isolates from the Great Lakes region of Africa (GLRA) spanning Burundi, the Democratic Republic of the Congo and Rwanda was assessed to better understand BBTV diversity and its epidemiology for improved disease management. DNA-R and DNA-S fragments of the virus genome were amplified and sequenced in this study. These two BBTV fragments were previously used to classify isolates into the South Pacific and the Asian groups. Phylogenetic analyses based on nucleotide sequences involving GLRA isolates and those obtained from the GenBank database were carried out. Sequence similarity for both DNA-R and DNA-S fragments ranged between 99.1 to 100.0% among the GLRA isolates, 96.2 to 100.0% and 89.7 to 94.3% between the GLRA isolates and those previously clustering in the South Pacific and the Asian groups, respectively. These results showed that GLRA isolates belong to the South Pacific group and are phylogenetically close to the reference Indian isolate. The similar banana cultivars and BBTV isolates across the GLRA implied that the disease may have mainly spread through exchange of planting material (suckers) between farmers. Thus, farmers' awareness and quarantine measures should be implemented to reduce BBTV spread in the GLRA.
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spelling CGSpace681422025-11-12T05:49:40Z Towards understanding the diversity of banana bunchy top virus in the Great Lakes region of Africa Niyongere, C. Lepoint, P.C.E. Losenge, T. Blomme, Guy Ateka, E.M. banana bunchy top virus plant viruses genomes pentalonia nigranervosa musa The genetic variability of banana bunchy top virus (BBTV) isolates from the Great Lakes region of Africa (GLRA) spanning Burundi, the Democratic Republic of the Congo and Rwanda was assessed to better understand BBTV diversity and its epidemiology for improved disease management. DNA-R and DNA-S fragments of the virus genome were amplified and sequenced in this study. These two BBTV fragments were previously used to classify isolates into the South Pacific and the Asian groups. Phylogenetic analyses based on nucleotide sequences involving GLRA isolates and those obtained from the GenBank database were carried out. Sequence similarity for both DNA-R and DNA-S fragments ranged between 99.1 to 100.0% among the GLRA isolates, 96.2 to 100.0% and 89.7 to 94.3% between the GLRA isolates and those previously clustering in the South Pacific and the Asian groups, respectively. These results showed that GLRA isolates belong to the South Pacific group and are phylogenetically close to the reference Indian isolate. The similar banana cultivars and BBTV isolates across the GLRA implied that the disease may have mainly spread through exchange of planting material (suckers) between farmers. Thus, farmers' awareness and quarantine measures should be implemented to reduce BBTV spread in the GLRA. 2015 2015-09-15T08:21:00Z 2015-09-15T08:21:00Z Journal Article https://hdl.handle.net/10568/68142 en Open Access application/pdf Academic Journals Niyongere, C.; Lepoint, P.; Losenge, T.; Blomme, G.; Ateka, E.M. (2015) Towards understanding the diversity of banana bunchy top virus in the Great Lakes region of Africa. African Journal of Agricultural Research 10(7) p. 702-709 ISSN: 1991-637X
spellingShingle banana bunchy top virus
plant viruses
genomes
pentalonia nigranervosa
musa
Niyongere, C.
Lepoint, P.C.E.
Losenge, T.
Blomme, Guy
Ateka, E.M.
Towards understanding the diversity of banana bunchy top virus in the Great Lakes region of Africa
title Towards understanding the diversity of banana bunchy top virus in the Great Lakes region of Africa
title_full Towards understanding the diversity of banana bunchy top virus in the Great Lakes region of Africa
title_fullStr Towards understanding the diversity of banana bunchy top virus in the Great Lakes region of Africa
title_full_unstemmed Towards understanding the diversity of banana bunchy top virus in the Great Lakes region of Africa
title_short Towards understanding the diversity of banana bunchy top virus in the Great Lakes region of Africa
title_sort towards understanding the diversity of banana bunchy top virus in the great lakes region of africa
topic banana bunchy top virus
plant viruses
genomes
pentalonia nigranervosa
musa
url https://hdl.handle.net/10568/68142
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