Evaluation of four different strategies to characterize plasma membrane proteins from banana roots
Plasma membrane proteins constitute a very important class of proteins. They are involved in the transmission of external signals to the interior of the cell and selective transport of water, nutrients and ions across the plasma membrane. However, the study of plasma membrane proteins is challenging...
| Autores principales: | , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
FapUNIFESP
2014
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/66053 |
| _version_ | 1855531035375173632 |
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| author | García, S. Panis, Bartholomeus Swennen, Rony L. Carpentier, Sebastien C. |
| author_browse | Carpentier, Sebastien C. García, S. Panis, Bartholomeus Swennen, Rony L. |
| author_facet | García, S. Panis, Bartholomeus Swennen, Rony L. Carpentier, Sebastien C. |
| author_sort | García, S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Plasma membrane proteins constitute a very important class of proteins. They are involved in the transmission of external signals to the interior of the cell and selective transport of water, nutrients and ions across the plasma membrane. However, the study of plasma membrane proteins is challenging because of their poor solubility in aqueous media and low relative abundance. In this work, we evaluated four different strategies for the characterization of plasma membrane proteins from banana roots: (i) the aqueous-polymer two-phase system technique (ATPS) coupled to gelelectrophoresis (gel-based), and (ii) ATPS coupled to LC-MS/MS (gel free), (iii) a microsomal fraction and (iv) a full proteome, both coupled to LC-MS/ MS. Our results show that the gel-based strategy is useful for protein visualization but has major limitations in terms of time reproducibility and efficiency. From the gel-free strategies, the microsomal-based strategy allowed the highest number of plasma membrane proteins to be identified, followed by the full proteome strategy and by the ATPS based strategy. The high yield of plasma membrane proteins provided by the microsomal fraction can be explained by the enrichment of membrane proteins in this fraction and the high throughput of the gel-free approach combined with the usage of a fast high-resolution mass spectrometer for the identification of proteins. |
| format | Journal Article |
| id | CGSpace66053 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | FapUNIFESP |
| publisherStr | FapUNIFESP |
| record_format | dspace |
| spelling | CGSpace660532025-11-12T05:44:37Z Evaluation of four different strategies to characterize plasma membrane proteins from banana roots García, S. Panis, Bartholomeus Swennen, Rony L. Carpentier, Sebastien C. bananas cell membranes identification methods musaceae plant protein roots Plasma membrane proteins constitute a very important class of proteins. They are involved in the transmission of external signals to the interior of the cell and selective transport of water, nutrients and ions across the plasma membrane. However, the study of plasma membrane proteins is challenging because of their poor solubility in aqueous media and low relative abundance. In this work, we evaluated four different strategies for the characterization of plasma membrane proteins from banana roots: (i) the aqueous-polymer two-phase system technique (ATPS) coupled to gelelectrophoresis (gel-based), and (ii) ATPS coupled to LC-MS/MS (gel free), (iii) a microsomal fraction and (iv) a full proteome, both coupled to LC-MS/ MS. Our results show that the gel-based strategy is useful for protein visualization but has major limitations in terms of time reproducibility and efficiency. From the gel-free strategies, the microsomal-based strategy allowed the highest number of plasma membrane proteins to be identified, followed by the full proteome strategy and by the ATPS based strategy. The high yield of plasma membrane proteins provided by the microsomal fraction can be explained by the enrichment of membrane proteins in this fraction and the high throughput of the gel-free approach combined with the usage of a fast high-resolution mass spectrometer for the identification of proteins. 2014-10 2015-05-13T13:59:53Z 2015-05-13T13:59:53Z Journal Article https://hdl.handle.net/10568/66053 en Open Access application/pdf FapUNIFESP Garcia, S.; Panis, B.; Swennen, R.; Carpentier, S. -2014-Evaluation of four different strategies to characterize plasma membrane proteins from banana roots-Ciencia e Agrotecnologia 38(5) -p. 424-434 |
| spellingShingle | bananas cell membranes identification methods musaceae plant protein roots García, S. Panis, Bartholomeus Swennen, Rony L. Carpentier, Sebastien C. Evaluation of four different strategies to characterize plasma membrane proteins from banana roots |
| title | Evaluation of four different strategies to characterize plasma membrane proteins from banana roots |
| title_full | Evaluation of four different strategies to characterize plasma membrane proteins from banana roots |
| title_fullStr | Evaluation of four different strategies to characterize plasma membrane proteins from banana roots |
| title_full_unstemmed | Evaluation of four different strategies to characterize plasma membrane proteins from banana roots |
| title_short | Evaluation of four different strategies to characterize plasma membrane proteins from banana roots |
| title_sort | evaluation of four different strategies to characterize plasma membrane proteins from banana roots |
| topic | bananas cell membranes identification methods musaceae plant protein roots |
| url | https://hdl.handle.net/10568/66053 |
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