Cryopreservation of Byrsonima intermedia embryos followed by room temperature thawing
Byrsonima intermedia is a shrub from the Brazilian Cerrado with medicinal properties. The storage of biological material at ultra-low temperatures (-196°C) is termed cryopreservation and represents a promising technique for preserving plant diversity. Thawing is a crucial step that follows cryoprese...
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| Format: | Journal Article |
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Universidade Estadual de Maringa
2014
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| Online Access: | https://hdl.handle.net/10568/66046 |
| _version_ | 1855531678921916416 |
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| author | Silva, L.C. Paiva, R. Swennen, Rony L. André, Edwige Panis, Bartholomeus |
| author_browse | André, Edwige Paiva, R. Panis, Bartholomeus Silva, L.C. Swennen, Rony L. |
| author_facet | Silva, L.C. Paiva, R. Swennen, Rony L. André, Edwige Panis, Bartholomeus |
| author_sort | Silva, L.C. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Byrsonima intermedia is a shrub from the Brazilian Cerrado with medicinal properties. The storage of biological material at ultra-low temperatures (-196°C) is termed cryopreservation and represents a promising technique for preserving plant diversity. Thawing is a crucial step that follows cryopreservation. The aim of this work was to cryopreserve B. intermedia zygotic embryos and subsequently thaw them at room temperature in a solution rich in sucrose. The embryos were decontaminated and desiccated in a laminar airflow hood for 0-4 hours prior to plunging into liquid nitrogen. The embryo moisture content (% MC) during dehydration was assessed. Cryopreserved embryos were thawed in a solution rich in sucrose at room temperature, inoculated in a germination medium and maintained in a growth chamber. After 30 days, the embryo germination was evaluated. No significant differences were observed between the different embryo dehydration times, where they were dehydrated for at least one hour. Embryos with a MC between 34.3 and 20.3% were germinated after cryopreservation. In the absence of dehydration, all embryos died following cryopreservation. We conclude that B. intermedia zygotic embryos can be successfully cryopreserved and thawed at room temperature after at least one hour of dehydration in a laminar airflow bench. |
| format | Journal Article |
| id | CGSpace66046 |
| institution | CGIAR Consortium |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Universidade Estadual de Maringa |
| publisherStr | Universidade Estadual de Maringa |
| record_format | dspace |
| spelling | CGSpace660462025-11-12T05:44:50Z Cryopreservation of Byrsonima intermedia embryos followed by room temperature thawing Silva, L.C. Paiva, R. Swennen, Rony L. André, Edwige Panis, Bartholomeus biological preservation dehydration freezing germination medicinal properties moisture content plant embryos thawing Byrsonima intermedia is a shrub from the Brazilian Cerrado with medicinal properties. The storage of biological material at ultra-low temperatures (-196°C) is termed cryopreservation and represents a promising technique for preserving plant diversity. Thawing is a crucial step that follows cryopreservation. The aim of this work was to cryopreserve B. intermedia zygotic embryos and subsequently thaw them at room temperature in a solution rich in sucrose. The embryos were decontaminated and desiccated in a laminar airflow hood for 0-4 hours prior to plunging into liquid nitrogen. The embryo moisture content (% MC) during dehydration was assessed. Cryopreserved embryos were thawed in a solution rich in sucrose at room temperature, inoculated in a germination medium and maintained in a growth chamber. After 30 days, the embryo germination was evaluated. No significant differences were observed between the different embryo dehydration times, where they were dehydrated for at least one hour. Embryos with a MC between 34.3 and 20.3% were germinated after cryopreservation. In the absence of dehydration, all embryos died following cryopreservation. We conclude that B. intermedia zygotic embryos can be successfully cryopreserved and thawed at room temperature after at least one hour of dehydration in a laminar airflow bench. 2014 2015-05-13T13:59:52Z 2015-05-13T13:59:52Z Journal Article https://hdl.handle.net/10568/66046 Open Access application/pdf Universidade Estadual de Maringa Silva, L.C.; Paiva, R.; Swennen, R.; Andre, E.; Panis, B. -2014-Cryopreservation of Byrsonima intermedia embryos followed by room temperature thawing-Acta Scientiarum Agronomy 36(3)-p. 309-315 |
| spellingShingle | biological preservation dehydration freezing germination medicinal properties moisture content plant embryos thawing Silva, L.C. Paiva, R. Swennen, Rony L. André, Edwige Panis, Bartholomeus Cryopreservation of Byrsonima intermedia embryos followed by room temperature thawing |
| title | Cryopreservation of Byrsonima intermedia embryos followed by room temperature thawing |
| title_full | Cryopreservation of Byrsonima intermedia embryos followed by room temperature thawing |
| title_fullStr | Cryopreservation of Byrsonima intermedia embryos followed by room temperature thawing |
| title_full_unstemmed | Cryopreservation of Byrsonima intermedia embryos followed by room temperature thawing |
| title_short | Cryopreservation of Byrsonima intermedia embryos followed by room temperature thawing |
| title_sort | cryopreservation of byrsonima intermedia embryos followed by room temperature thawing |
| topic | biological preservation dehydration freezing germination medicinal properties moisture content plant embryos thawing |
| url | https://hdl.handle.net/10568/66046 |
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