Phenotypic stability and genome wide association study of late blight resistance in potato genotypes adapted to the tropical highlands

Potato genotypes from a breeding population adapted to tropical highlands were analyzed for the stability of late blight resistance and also for marker-phenotype association. We harmonized the historical evaluation data, consisting of observations spanning 6 years from two field sites utilizing a re...

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Main Authors: Lindqvist-Kreuze, H., Gastelo, M., Pérez, W., Forbes, G., Koeyer, D. de, Bonierbale, Merideth W.
Format: Journal Article
Language:Inglés
Published: Scientific Societies 2014
Subjects:
Online Access:https://hdl.handle.net/10568/64879
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author Lindqvist-Kreuze, H.
Gastelo, M.
Pérez, W.
Forbes, G.
Koeyer, D. de
Bonierbale, Merideth W.
author_browse Bonierbale, Merideth W.
Forbes, G.
Gastelo, M.
Koeyer, D. de
Lindqvist-Kreuze, H.
Pérez, W.
author_facet Lindqvist-Kreuze, H.
Gastelo, M.
Pérez, W.
Forbes, G.
Koeyer, D. de
Bonierbale, Merideth W.
author_sort Lindqvist-Kreuze, H.
collection Repository of Agricultural Research Outputs (CGSpace)
description Potato genotypes from a breeding population adapted to tropical highlands were analyzed for the stability of late blight resistance and also for marker-phenotype association. We harmonized the historical evaluation data, consisting of observations spanning 6 years from two field sites utilizing a resistance scale constructed by comparing the area under the disease progress curve (AUDPC) values of 172 genotypes with that of susceptible control ‘Yungay’. In total, 70 potato genotypes had a coefficient of variability <0.5 and were considered stable across the environments tested. A principal component analysis demonstrated that the ensemble of experiments formed two distinct groups that reflect the stability of genotype resistance to late blight. Phytophthora infestans isolates present in the experimental fields belonged to the EC-1 clonal lineage and showed variation in virulence beyond the concept of the avirulence determined by the conventionally used R1-R11 differential set. A single-nucleotide polymorphism (SNP) marker on chromosome 9 was associated with late blight resistance and linked to instability. Genotypes with either AACC or AAAC combinations for this SNP were highly resistant only in some environments, while the genotypes with the AAAA combination had more moderate levels of resistance but were stable across environments.
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spelling CGSpace648792024-05-01T08:20:11Z Phenotypic stability and genome wide association study of late blight resistance in potato genotypes adapted to the tropical highlands Lindqvist-Kreuze, H. Gastelo, M. Pérez, W. Forbes, G. Koeyer, D. de Bonierbale, Merideth W. potatoes phytophthora infestans genotypes highlands tropical zones Potato genotypes from a breeding population adapted to tropical highlands were analyzed for the stability of late blight resistance and also for marker-phenotype association. We harmonized the historical evaluation data, consisting of observations spanning 6 years from two field sites utilizing a resistance scale constructed by comparing the area under the disease progress curve (AUDPC) values of 172 genotypes with that of susceptible control ‘Yungay’. In total, 70 potato genotypes had a coefficient of variability <0.5 and were considered stable across the environments tested. A principal component analysis demonstrated that the ensemble of experiments formed two distinct groups that reflect the stability of genotype resistance to late blight. Phytophthora infestans isolates present in the experimental fields belonged to the EC-1 clonal lineage and showed variation in virulence beyond the concept of the avirulence determined by the conventionally used R1-R11 differential set. A single-nucleotide polymorphism (SNP) marker on chromosome 9 was associated with late blight resistance and linked to instability. Genotypes with either AACC or AAAC combinations for this SNP were highly resistant only in some environments, while the genotypes with the AAAA combination had more moderate levels of resistance but were stable across environments. 2014-06 2015-04-02T11:47:57Z 2015-04-02T11:47:57Z Journal Article https://hdl.handle.net/10568/64879 en Open Access Scientific Societies Lindqvist-Kreuze, H.; Gastelo, M.; Perez, W.; Forbes, G. A.; Koeyer, D. de.; Bonierbale, M. 2014. Phenotypic stability and genome wide association study of late blight resistance in potato genotypes adapted to the tropical highlands. Phytopathology. (USA). ISSN 0031-949X. 104(6):624-633.
spellingShingle potatoes
phytophthora infestans
genotypes
highlands
tropical zones
Lindqvist-Kreuze, H.
Gastelo, M.
Pérez, W.
Forbes, G.
Koeyer, D. de
Bonierbale, Merideth W.
Phenotypic stability and genome wide association study of late blight resistance in potato genotypes adapted to the tropical highlands
title Phenotypic stability and genome wide association study of late blight resistance in potato genotypes adapted to the tropical highlands
title_full Phenotypic stability and genome wide association study of late blight resistance in potato genotypes adapted to the tropical highlands
title_fullStr Phenotypic stability and genome wide association study of late blight resistance in potato genotypes adapted to the tropical highlands
title_full_unstemmed Phenotypic stability and genome wide association study of late blight resistance in potato genotypes adapted to the tropical highlands
title_short Phenotypic stability and genome wide association study of late blight resistance in potato genotypes adapted to the tropical highlands
title_sort phenotypic stability and genome wide association study of late blight resistance in potato genotypes adapted to the tropical highlands
topic potatoes
phytophthora infestans
genotypes
highlands
tropical zones
url https://hdl.handle.net/10568/64879
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