Transcriptome analysis and systemic RNAi response in the African sweetpotato weevil (Cylas puncticollis, Coleoptera, Brentidae
The African sweetpotato weevil (SPW) Cylas puncticollis Boheman is one of the most important constraints of sweetpotato production in Sub-Saharan Africa and yet is largely an uncharacterized insect pest. Here, we report on the transcriptome analysis of SPW generated using an Illumina platform. More...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Public Library of Science
2015
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| Online Access: | https://hdl.handle.net/10568/58303 |
| _version_ | 1855521718171336704 |
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| author | Prentice, K. Pertry, I. Christiaens, O. Bauters, L. Bailey, M. Niblett, C. Ghislain, M. Gheysen, G. Smagghe, G. |
| author_browse | Bailey, M. Bauters, L. Christiaens, O. Gheysen, G. Ghislain, M. Niblett, C. Pertry, I. Prentice, K. Smagghe, G. |
| author_facet | Prentice, K. Pertry, I. Christiaens, O. Bauters, L. Bailey, M. Niblett, C. Ghislain, M. Gheysen, G. Smagghe, G. |
| author_sort | Prentice, K. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The African sweetpotato weevil (SPW) Cylas puncticollis Boheman is one of the most important constraints of sweetpotato production in Sub-Saharan Africa and yet is largely an uncharacterized insect pest. Here, we report on the transcriptome analysis of SPW generated using an Illumina platform. More than 213 million sequencing reads were obtained and assembled into 89,599 contigs. This assembly was followed by a gene ontology annotation. Subsequently, a transcriptome search showed that the necessary RNAi components relevant to the three major RNAi pathways, were found to be expressed in SPW. To address the functionality of the RNAi mechanism in this species, dsRNA was injected into second instar larvae targeting laccase2, a gene which encodes an enzyme involved in the sclerotization of insect exoskeleton. The body of treated insects showed inhibition of sclerotization, leading eventually to death. Quantitative Real Time PCR (qPCR) confirmed this phenotype to be the result of gene silencing. Together, our results provide valuable sequence data on this important insect pest and demonstrate that a functional RNAi pathway with a strong and systemic effect is present in SPW and can further be explored as a new strategy for controlling this important pest. |
| format | Journal Article |
| id | CGSpace58303 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Public Library of Science |
| publisherStr | Public Library of Science |
| record_format | dspace |
| spelling | CGSpace583032025-11-06T14:05:21Z Transcriptome analysis and systemic RNAi response in the African sweetpotato weevil (Cylas puncticollis, Coleoptera, Brentidae Prentice, K. Pertry, I. Christiaens, O. Bauters, L. Bailey, M. Niblett, C. Ghislain, M. Gheysen, G. Smagghe, G. sweet potatoes cylas rna sequence pcr pest insects The African sweetpotato weevil (SPW) Cylas puncticollis Boheman is one of the most important constraints of sweetpotato production in Sub-Saharan Africa and yet is largely an uncharacterized insect pest. Here, we report on the transcriptome analysis of SPW generated using an Illumina platform. More than 213 million sequencing reads were obtained and assembled into 89,599 contigs. This assembly was followed by a gene ontology annotation. Subsequently, a transcriptome search showed that the necessary RNAi components relevant to the three major RNAi pathways, were found to be expressed in SPW. To address the functionality of the RNAi mechanism in this species, dsRNA was injected into second instar larvae targeting laccase2, a gene which encodes an enzyme involved in the sclerotization of insect exoskeleton. The body of treated insects showed inhibition of sclerotization, leading eventually to death. Quantitative Real Time PCR (qPCR) confirmed this phenotype to be the result of gene silencing. Together, our results provide valuable sequence data on this important insect pest and demonstrate that a functional RNAi pathway with a strong and systemic effect is present in SPW and can further be explored as a new strategy for controlling this important pest. 2015 2015-03-13T21:08:27Z 2015-03-13T21:08:27Z Journal Article https://hdl.handle.net/10568/58303 en Open Access application/pdf Public Library of Science Prentice, K.; Pertry, I.; Christiaens, O.; Bauters, L.; Bailey, A.; Niblett, C.; Ghislain, M.; Gheysen, G.; Smagghe, G. 2015. Transcriptome analysis and systemic RNAi response in the African sweetpotato weevil (Cylas puncticollis, Coleoptera, Brentidae). PLoS ONE. (USA). ISSN 1932-6203. 10(1):e0115336. |
| spellingShingle | sweet potatoes cylas rna sequence pcr pest insects Prentice, K. Pertry, I. Christiaens, O. Bauters, L. Bailey, M. Niblett, C. Ghislain, M. Gheysen, G. Smagghe, G. Transcriptome analysis and systemic RNAi response in the African sweetpotato weevil (Cylas puncticollis, Coleoptera, Brentidae |
| title | Transcriptome analysis and systemic RNAi response in the African sweetpotato weevil (Cylas puncticollis, Coleoptera, Brentidae |
| title_full | Transcriptome analysis and systemic RNAi response in the African sweetpotato weevil (Cylas puncticollis, Coleoptera, Brentidae |
| title_fullStr | Transcriptome analysis and systemic RNAi response in the African sweetpotato weevil (Cylas puncticollis, Coleoptera, Brentidae |
| title_full_unstemmed | Transcriptome analysis and systemic RNAi response in the African sweetpotato weevil (Cylas puncticollis, Coleoptera, Brentidae |
| title_short | Transcriptome analysis and systemic RNAi response in the African sweetpotato weevil (Cylas puncticollis, Coleoptera, Brentidae |
| title_sort | transcriptome analysis and systemic rnai response in the african sweetpotato weevil cylas puncticollis coleoptera brentidae |
| topic | sweet potatoes cylas rna sequence pcr pest insects |
| url | https://hdl.handle.net/10568/58303 |
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