Improved cryopreservation method for the long-term conservation of the world potato germplasm collection
The effect of cold and sucrose pretreatment for increasing tolerance to cryopreservation was evaluated with eight diverse genotypes, six cultivars belonging to the cultivated species, Solanum tuberosum spp., S. tuberosum subsp. andigena, S. x juzepczukii and S. x ajanhuiri, and two genotypes from th...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2015
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/58302 |
| _version_ | 1855529010329550848 |
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| author | Panta, A. Panis, Bartholomeus Ynouye, C. Swennen, Rony L. Roca, W. Tay, D. Ellis, David |
| author_browse | Ellis, David Panis, Bartholomeus Panta, A. Roca, W. Swennen, Rony L. Tay, D. Ynouye, C. |
| author_facet | Panta, A. Panis, Bartholomeus Ynouye, C. Swennen, Rony L. Roca, W. Tay, D. Ellis, David |
| author_sort | Panta, A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The effect of cold and sucrose pretreatment for increasing tolerance to cryopreservation was evaluated with eight diverse genotypes, six cultivars belonging to the cultivated species, Solanum tuberosum spp., S. tuberosum subsp. andigena, S. x juzepczukii and S. x ajanhuiri, and two genotypes from the wild species, S. commersonii. In vitro plantlets were cultured at either 6 or 22 °C in media supplemented with either 0.07 or 0.3 M sucrose prior to droplet PVS2 cryopreservation. The sucrose pretreatment appeared to have no positive effect on post-cryo survival. The cold-hardening pretreatment increased significantly post-cryo recovery in drought and frost tolerant cultivars. When 755 accessions, representing 10 taxa, were cryopreserved after cold-hardening, 96 % responded with at least one shoot recovering and 63 % showed a high recovery rate (40–100 %). Therefore this method is recommended for the long term conservation of diverse accessions of potato germplasm. |
| format | Journal Article |
| id | CGSpace58302 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace583022025-11-12T05:43:32Z Improved cryopreservation method for the long-term conservation of the world potato germplasm collection Panta, A. Panis, Bartholomeus Ynouye, C. Swennen, Rony L. Roca, W. Tay, D. Ellis, David potatoes solanum commersonii varieties wild plants germplasm collections germplasm conservation gene banks cryopreservation abiotic stress The effect of cold and sucrose pretreatment for increasing tolerance to cryopreservation was evaluated with eight diverse genotypes, six cultivars belonging to the cultivated species, Solanum tuberosum spp., S. tuberosum subsp. andigena, S. x juzepczukii and S. x ajanhuiri, and two genotypes from the wild species, S. commersonii. In vitro plantlets were cultured at either 6 or 22 °C in media supplemented with either 0.07 or 0.3 M sucrose prior to droplet PVS2 cryopreservation. The sucrose pretreatment appeared to have no positive effect on post-cryo survival. The cold-hardening pretreatment increased significantly post-cryo recovery in drought and frost tolerant cultivars. When 755 accessions, representing 10 taxa, were cryopreserved after cold-hardening, 96 % responded with at least one shoot recovering and 63 % showed a high recovery rate (40–100 %). Therefore this method is recommended for the long term conservation of diverse accessions of potato germplasm. 2015-01 2015-03-13T19:39:26Z 2015-03-13T19:39:26Z Journal Article https://hdl.handle.net/10568/58302 en Open Access application/pdf Springer Panta, A.; Panis, B.; Ynouye, C.; Swennen, R.; Roca, W.; Tay, D.; Ellis, D. 2015. Improved cryopreservation method for the long-term conservation of the world potato germplasm collection. Plant Cell, Tissue and Organ Culture. 120(1):117-125. |
| spellingShingle | potatoes solanum commersonii varieties wild plants germplasm collections germplasm conservation gene banks cryopreservation abiotic stress Panta, A. Panis, Bartholomeus Ynouye, C. Swennen, Rony L. Roca, W. Tay, D. Ellis, David Improved cryopreservation method for the long-term conservation of the world potato germplasm collection |
| title | Improved cryopreservation method for the long-term conservation of the world potato germplasm collection |
| title_full | Improved cryopreservation method for the long-term conservation of the world potato germplasm collection |
| title_fullStr | Improved cryopreservation method for the long-term conservation of the world potato germplasm collection |
| title_full_unstemmed | Improved cryopreservation method for the long-term conservation of the world potato germplasm collection |
| title_short | Improved cryopreservation method for the long-term conservation of the world potato germplasm collection |
| title_sort | improved cryopreservation method for the long term conservation of the world potato germplasm collection |
| topic | potatoes solanum commersonii varieties wild plants germplasm collections germplasm conservation gene banks cryopreservation abiotic stress |
| url | https://hdl.handle.net/10568/58302 |
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